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We narrowed to 216 results for: Sleeping Beauty

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Showing: 151 - 200 of 216 results

  1. pTS2340_Tier3(SB)-iRFP_Puro

    Plasmid
    #169638
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven iRFP670 and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    169638-plasmid-map-sequence-id-364985

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  2. pTS2362_Tier3(SB)-rtTA-PuroR

    Plasmid
    #169658
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169658-plasmid-map-sequence-id-365631

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  3. EGFP in pDBEcR-pIND(SP1)-DD/Bla

    Plasmid
    #201559
    Purpose
    Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD and DBEcR (3xmyc-tagged); blasticidin resistance.
    Depositor
    Randy Poon
    Article
    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754
    Insert
    DBEcR and EGFP
    Use
    Unspecified; Sleeping beauty transposon
    Tags
    DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD
    Expression
    Mammalian
    Promoter
    EF1a and pIND(SP1)
    Available Since
    July 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    201559-plasmid-map-sequence-id-400941

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  4. EGFP in pDBEcR-pIND(SP1)-W/Bla

    Plasmid
    #201558
    Purpose
    Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.
    Depositor
    Randy Poon
    Article
    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754
    Insert
    DBEcR and EGFP
    Use
    Unspecified; Sleeping beauty transposon
    Tags
    DBEcR-myc and FLAG-EGFP
    Expression
    Mammalian
    Promoter
    EF1a and pIND(SP1)
    Available Since
    July 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    201558-plasmid-map-sequence-id-400924

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  5. pTS2337_Tier3(SB)-TagBFP2_Puro

    Plasmid
    #169635
    Purpose
    Tier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase including a TagBFP marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven mTagBFP2 and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    169635-plasmid-map-sequence-id-366786

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  9. pTS2343_Tier3(SB)-YPet_Blast

    Plasmid
    #169641
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-BlastR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven YPet and BlastR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169641-plasmid-map-sequence-id-366220

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  10. pTS2344_Tier3(SB)-mRuby2_Blast

    Plasmid
    #169642
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-BlastR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven mRuby2 and BlastR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169642-plasmid-map-sequence-id-366219

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  11. pTS2339_Tier3(SB)-Ruby_Puro

    Plasmid
    #169637
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven mRuby2 and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169637-plasmid-map-sequence-id-366201

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  17. EGFP in pDBEcR-pIND(SP1)-DD-W/Bla

    Plasmid
    #201560
    Purpose
    Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.
    Depositor
    Randy Poon
    Article
    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754
    Insert
    DBEcR and EGFP
    Use
    Unspecified; Sleeping beauty transposon
    Tags
    DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD
    Expression
    Mammalian
    Promoter
    EF1a and pIND(SP1)
    Available Since
    July 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    201560-plasmid-map-sequence-id-400950

    Enlarge

  19. pTS2361_Tier3(SB)-rtTA-YPet_PuroR

    Plasmid
    #169657
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169657-plasmid-map-sequence-id-365632

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  20. pNoROS-CAT

    Plasmid
    #170152
    Purpose
    Mammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidation
    Depositor
    Dimitri Pestov
    Article
    Sapio et al Front Mol Biosci. 2021 Apr 26;8:678488.
    Inserts
    catalase (Cat Mouse)
    roGFP2
    Use
    Genome integration via sleeping beauty transposon…
    Tags
    FLAG tag and Nucleolar Localization Sequence (NoL…
    Expression
    Mammalian
    Promoter
    RPBSA and TRE promoter
    Available Since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170152-plasmid-map-sequence-id-335808

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  24. pAF92

    Plasmid
    #135929
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil mCherry-TNFSF9 (type II transmembrane) fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    mCherry fused to TNFSF9
    Use
    Transposon
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135929-plasmid-map-sequence-id-270167

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  25. pAF197

    Plasmid
    #135930
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil CTLA4-Fc-mCherry fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. CTLA4).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    CTLA4 fused to IgG Fc and to mCherry
    Use
    Transposon
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135930-plasmid-map-sequence-id-270077

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  26. pAF139

    Plasmid
    #135924
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mTagBFP fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mTagBFP
    Use
    Transposon
    Tags
    mTagBFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135924-plasmid-map-sequence-id-270080

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  27. pAF164

    Plasmid
    #135928
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mOrange fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mOrange
    Use
    Transposon
    Tags
    mOrange
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135928-plasmid-map-sequence-id-270165

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  29. pAF160

    Plasmid
    #135925
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mAzurite fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mAzurite
    Use
    Transposon
    Tags
    mAzurite
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135925-plasmid-map-sequence-id-270082

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  30. pAF138

    Plasmid
    #135923
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCitrine fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mCitrine
    Use
    Transposon
    Tags
    mCitrine
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135923-plasmid-map-sequence-id-270125

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  31. pAF161

    Plasmid
    #135926
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCerulean3 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mCerulean3
    Use
    Transposon
    Tags
    mCerulean3
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135926-plasmid-map-sequence-id-270079

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  32. pAF163

    Plasmid
    #135927
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mNeptune2 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mNeptune2
    Use
    Transposon
    Tags
    mNeptune2
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135927-plasmid-map-sequence-id-270084

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  41. LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR

    Plasmid
    #68345
    Purpose
    A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii
    Inserts
    puromycin
    hCas9
    Use
    CRISPR; Flp/frt
    Tags
    linked to red flouresent protein gene mKateII and…
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68345-plasmid-map-sequence-id-438782

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  42. pLP-PIGA3 (pLP305)

    Plasmid
    #196992
    Purpose
    Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites, sleeping beauty ITRs and BsmBI homology arms cloning sites.
    Depositor
    Matthew Maurano
    Article
    Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    196992-plasmid-map-sequence-id-389805

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  45. pT3-EF1aH-PIK3CA H1047R

    Plasmid
    #180031
    Purpose
    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.
    Depositor
    Xin Chen
    Article
    Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv
    Insert
    PIK3CA H1047R (PIK3CA Human)
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    H1047R
    Promoter
    EF1a
    Available Since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180031-plasmid-map-sequence-id-357864

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  49. pSbi-pur-EGFP-KSR1-CA3 (N-KSR)

    Plasmid
    #217769
    Purpose
    Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)
    Depositor
    Paula Nunes-Hasler
    Article
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Sleeping beauty transposase-compatible genome ins…
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CA3 domain aa 317-400
    Promoter
    EF-1alpha promoter
    Available Since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217769-plasmid-map-sequence-id-435786

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  50. pSbi-pur-KSR1-CA3-EGFP (C-KSR)

    Plasmid
    #217768
    Purpose
    Fluorescent reporter for ceramide (for stable mammalian expression)
    Depositor
    Paula Nunes-Hasler
    Article
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Sleeping beauty transposase-compatible genome ins…
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CA3 domain aa 317-400 plus vector-based linker LE…
    Promoter
    EF-1alpha promoter
    Available Since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217768-plasmid-map-sequence-id-435794

    Enlarge

Showing: 151 - 200 of 216 results