We narrowed to 15,323 results for: pET
-
Plasmid#133621PurposeExpression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.DepositorAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pET28a-RBP-LOV2
Plasmid#185792PurposeEncodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linkerDepositorInsertRBP-LOV2
Tags10xHis, TEV protease cleavage site after His TagExpressionBacterialPromoterT7Available SinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-Sangamo-HIS
Plasmid#40787Purposefor e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokIDepositorInsertTranscription Activator Like Effector Nuclease
UseTALENTagsC Terminal 6xHISExpressionBacterialMutation+63 C Terminus,Codon Optimized FokI EndonucleasePromoterT7Available SinceDec. 12, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21-pelB-LaM-2
Plasmid#172768PurposeBacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-2
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b+H2B(T116C)
Plasmid#157808PurposeExpression of human histone H2B with T116C mutation to facilitate maleimide chemistryDepositorInsertH2B(T116C)
ExpressionBacterialMutationT116CPromoterT7Available SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
wt 6XHis-SNX9 pET15b
Plasmid#34690DepositorInsertSorting nexin 9
Tags6xHisExpressionBacterialAvailable SinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Tipin-His
Plasmid#23122DepositorAvailable SinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7REwt
Plasmid#213935PurposeGST tagged AO7 encoding aa 126-258 for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationAO7 cloned into the first multiple cloning site o…PromoterT7 promoter-1Available SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-Bst-LF-Idaho
Plasmid#170469PurposeExpresses His-tagged Bst-LF-Idaho in E. coliDepositorInsertBst-LF-Idaho
TagsN-terminal His-tagExpressionBacterialAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_05
Plasmid#194666PurposeProtein production of the split HaloTag based calcium recorder Caprola_05 in bacteriaDepositorInsertCaprola_05
TagsHis-tag and Strep-tagExpressionBacterialPromoterT7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HaloTag7SS-DogTag
Plasmid#171775PurposeExpresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formationDepositorInsertHaloTag7SS-DogTag
TagsHis6ExpressionBacterialMutationDogTag inserted in HaloTag7 between residues D139…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)::MBP-NL3F10H
Plasmid#141291PurposeMBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertMBP-NLUC3F10H
UseLuciferaseTagsFlag (x3), His (x10), and MBPExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-SpyCatcher002-MBP
Plasmid#216295PurposeExpresses SpyCatcher002 linked to maltose-binding protein in bacterial cellsDepositorInsertSpyCatcher002-MBP
TagsHis6 tag and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-AsCas12f
Plasmid#171612PurposeAsCas12f1 heterologous expression plasmidDepositorInsertAsCas12f1
ExpressionBacterialAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLicC-MBP-APETx2
Plasmid#72668PurposePeriplasmic expression of APETx2 in E.coliDepositorInsertAPETx2
TagsHis tag, MBP, MalE signal sequence for periplasmi…ExpressionBacterialPromoterT7Available SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM-11-6His-DmBelle_B
Plasmid#145950PurposeBacterial Expression of DmBelleDepositorInsertDmBelle (bel Fly)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a-C2m2-mKate
Plasmid#208765PurposeTo express C2m2-mKate in bacteriaDepositorInsertC2m2-mKate (Mfge8 Mouse)
TagsmKateExpressionBacterialMutationMutated 24 and 45 lysine to asparagine in C2 doma…PromoterT7 promoterAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_01
Plasmid#194662PurposeProtein production of the split HaloTag based calcium recorder Caprola_01 in bacteriaDepositorInsertCaprola_01
TagsHis-tag and Strep-tagExpressionBacterialPromoterT7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET sfGFP(150TAG)
Plasmid#133455Purposeexpresses sfGFP with an unnatural amino acid at the 150th position in bacterial cellsDepositorInsertsfGFP
TagsHisExpressionBacterialPromoterT7Available SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-30
Plasmid#172754PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-30
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-67/68
Plasmid#113922PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68DepositorInsertMBP-NanoLucCP-67/68
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-PfAgo_F
Plasmid#146270PurposeBacterial Expression of PfAgoDepositorInsertPfAgo
ExpressionBacterialMutationone non silent mutation A907G (I303V) and one sil…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b-His6-UBCH7
Plasmid#212711PurposeBacterial expression of His6-UBCH7DepositorInsertUBCH7
Tags6xHisExpressionBacterialAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a-hAIM2-His6
Plasmid#51532PurposeExpress His6-tagged hAIM2 in bacteriaDepositorAvailable SinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-Mff(1-61)-PP-GST
Plasmid#73042PurposeBacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage siteDepositorInsertMff(1-61) (Mff Mouse)
TagsGST-tag and PreScission protease siteExpressionBacterialMutationTruncation containing amino acids 1-61PromoterT7Available SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-His-sfGFP
Plasmid#180755PurposepET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
Tagsstrep tagExpressionBacterialAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_13
Plasmid#194674PurposeProtein production of the split HaloTag based calcium recorder Caprola_13 in bacteriaDepositorInsertCaprola_13
TagsHis-tag and Strep-tagExpressionBacterialPromoterT7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-cpHalo∆
Plasmid#194659PurposeProtein production of the split HaloTag part cpHalo∆ (cpHaloTag135/156) in bacteriaDepositorInsertcpHalo∆
TagsHis-tagExpressionBacterialPromoterT7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b LFn-Fla
Plasmid#84871PurposeBacterial expression of LFn-Fla fusionDepositorInsertsLFn
Fla
TagsHisExpressionBacterialPromoterT7Available SinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
VV001: WT Arch-3 in pET28b
Plasmid#58487PurposeExpresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coliDepositorInsertArchaerhodopsin-3 (with C-terminal His tag)
ExpressionBacterialPromoterT7Available SinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-hRanGAP1tail
Plasmid#53139PurposeE.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tagDepositorInsertRanGAP1 (RANGAP1 Human)
TagsHisExpressionBacterialMutationC-terminal fragment (amino acids 398-587)PromoterT7Available SinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 Flag-LplA
Plasmid#232118PurposeFor bacterial expression of Flag-tagged E. coli LplA.DepositorInsertlplA (lplA Escherichia coli (strain K12))
TagsFlagExpressionBacterialPromoterT7 PromoterAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-BCA-Q11
Plasmid#185781PurposeEncodes enzyme bovine carbonic anhydrase linked to Q11 self-assembling peptide via a GS-linker sequenceDepositorInsertBCA-Q11
ExpressionBacterialPromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-HRASLS3-1-132
Plasmid#100725PurposeExpression of N-terminal region of human HRASLS3 in E.coliDepositorAvailable SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-51b-HaloTag11
Plasmid#175519PurposeExpression of HaloTag11 in bacterial cellsDepositorInsertHaloTag11
TagsHisExpressionBacterialAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET3d-E2-25K-C170S
Plasmid#18892DepositorInsertUbiquitin conjugating enzyme E2-25K (UBE2K Most likely H. sapiens, Human)
ExpressionBacterialMutationCysteine 170 is replaced with a Serine residue.Available SinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET15b T7pCONS sfGFP
Plasmid#154467PurposepET15b with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCyPet-Rac1(T17N)
Plasmid#22784DepositorAvailable SinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-YTHDF2
Plasmid#204406PurposeE. coli expression of full-length YTHDF2 with an N-terminal mCherry tagDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-18
Plasmid#172763PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-18
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-1-mRhubarb720-HO
Plasmid#141201PurposeExpresses mRhubarb720 fluorescent protein and heme oxygenaseDepositorInsertsmRhubarb720
heme oxygenase
Tags6x-HisExpressionBacterialMutationR134H, L196Q, T202D, V203I, W309R, Q310A compared…PromoterT7Available SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b-CoilR-Cys
Plasmid#181989PurposeUsed to label proteins with the CoilE genetically encoded tagDepositorInsertCoilR-Cys
TagsHis-TagExpressionBacterialAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherry-2xEcMTS
Plasmid#133624PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.DepositorInsertmCherry-2xEcMTS
TagsHisExpressionBacterialAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-Gate2 ccdB
Plasmid#117116PurposeDestination vectorDepositorTypeEmpty backboneUseBackbone for golden gate assembly for gene target…Available SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mEGFP
Plasmid#185013PurposeBacterial Expression of 6xHis Sirius-GFP Fusion ProteinDepositorInsertSirius-GFP
ExpressionBacterialAvailable SinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-AqpZ-ALFA
Plasmid#212310PurposeExpression of AqpZ with ALFA tagDepositorInsertAquaporin Z
TagsALFA tag and Strep-Tag IIExpressionBacterialPromoterT7Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21-GFP-AURKA
Plasmid#99875PurposeExpression of GFP-AURKA for protein purification in bacteriaDepositorAvailable SinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a-hAIM2-GST
Plasmid#51528PurposeExpress GST-tagged hAIM2 in bacteriaDepositorAvailable SinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a-trAtCCD8(pYL735)
Plasmid#178286PurposeExpresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from ArabidopsisDepositorInserttrAtCCD8
ExpressionBacterialPromoterT7Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-10
Plasmid#172743PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-10
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only