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179,317 results

Showing: 8381 - 8400 of 179317 results

  1. pYPQ-dzCas9-Act3.0

    Plasmid
    #158414
    Purpose
    CRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Publication
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dzCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available since
    July 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    158414-plasmid-map-sequence-id-332091

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  2. pMasterK

    Plasmid
    #165081
    Purpose
    Episomal expression of reprogramming factors.
    Depositor
    Hideyuki Okano
    Publication
    Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    MYC (MYC Human)
    LRH1 (NR5A2 Human)
    NANOG (NANOG Human)
    lin28 (LIN28A Human)
    EGFP
    HSV TK
    KDM4D (KDM4D Callithrix jacchus)
    GLIS1 (GLIS1 Human)
    Use
    Episomal
    Expression
    Mammalian
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    165081-plasmid-map-sequence-id-514763

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  6. pETM33_Nsp5_Mpro

    Plasmid
    #156475
    Purpose
    Bacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Publication
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp5_Mpro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    156475-plasmid-map-sequence-id-307727

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  13. pGL4.22-PGK1-HRE::dLUC

    Plasmid
    #128095
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Publication
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Promoter
    inserted PGK1 hypoxia response elements (HRE) (x3)
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    128095-plasmid-map-sequence-id-249667

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  14. pGL4.22-VEGF-HRE::dLUC

    Plasmid
    #128096
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Publication
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Hypoxia response element (x5) from VEGF promoter (VEGFA Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Promoter
    inserted VEGF hypoxia response elements (HRE) (x5)
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    128096-plasmid-map-sequence-id-249669

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  18. pET29b-H6_Streptavidin_sfGFP

    Plasmid
    #124296
    Purpose
    His6-tagged Streptavidin GFP fusion protein
    Depositor
    Mark Arbing
    Insert
    His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
    Tags
    His6 and T7 tags
    Expression
    Bacterial
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124296-plasmid-map-sequence-id-240453

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  19. mNeonGreen-2A-mTurquoise2

    Plasmid
    #98885
    Purpose
    Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
    Depositor
    Dorus Gadella
    Publication
    Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mNeonGreen-2A-mTurquoise2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    98885-plasmid-map-sequence-id-191168

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Showing: 8381 - 8400 of 179317 results