166,034 results

1. CAG FUCCI

   Plasmid

   #175266

   Purpose

   Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis

   Depositor

   James Monaghan

   Article

   Duerr et al Development. 2022 Mar 15;149(6). pii: 27

   Insert

   FUCCI
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine

   Plasmid

   #50463

   Purpose

   Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Insert

   hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
   

   Use

   AAV

   Tags

   HA

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. PresentER-SIINFEKL (mCherry)

   Plasmid

   #102945

   Purpose

   PresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)

   Depositor

   David Scheinberg

   Article

   Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

   Insert

   SIINFEKL (OVAL Synthetic, Chicken)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   OVA257-264

   Promoter

   LTR

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pCold-I-Dsup

   Plasmid

   #90021

   Purpose

   To express His-tagged Dsup in bacteria

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   cspA

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-EF1a-i53

   Plasmid

   #216243

   Purpose

   Express i53 in mammalian cells as a HDR enhancer

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   i53
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB551

   Plasmid

   #74881

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFPC1-hVAP-B

   Plasmid

   #104448

   Purpose

   Expression of human VAP-B fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein B (VAPB Human)
   

   Tags

   EGFP and HA

   Expression

   Mammalian

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-TFEBS211A-MYC (#805)

   Plasmid

   #99957

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

   Insert

   TFEB (TFEB Human)
   

   Tags

   6xHIs and Myc

   Expression

   Mammalian

   Mutation

   S211A

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST14-Cys-DogCatcher

   Plasmid

   #171929

   Purpose

   Express Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   Cys-DogCatcher
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB402SC

    Plasmid

    #224552

    Purpose

    Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.

    Depositor

    Paul Schulze-Lefert

    Article

    Lawson et al bioRxiv 2024.07.14.603419

    Type

    Empty backbone

    Tags

    Twin-Strep-HA

    Expression

    Plant

    Promoter

    2 X 35S

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWB402SN

    Plasmid

    #224551

    Purpose

    Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.

    Depositor

    Paul Schulze-Lefert

    Article

    Lawson et al bioRxiv 2024.07.14.603419

    Type

    Empty backbone

    Tags

    HA-Twin-Strep

    Expression

    Plant

    Promoter

    2 X 35S

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

    Plasmid

    #164514

    Purpose

    Green glucose sensor with mRuby2 fusion, cytosolic

    Depositor

    Loren Looger

    Article

    Keller et al bioRxiv (2019) 571422; doi: https://doi.

    Insert

    (Cyto)iGlucoSnFR-mRuby2
    

    Use

    AAV

    Mutation

    Synthetic Construct

    Promoter

    GFAP

    Available Since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUltra-puro-RTTA3

    Plasmid

    #58750

    Purpose

    silence doxycyclin inducible pUltra-dox

    Depositor

    Yildirim Dogan, Kitai Kim

    Insert

    Reverse TET-Transactivator 3
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    loxP5171

    Promoter

    Ubiquitin C promoter

    Available Since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-CDK4

    Plasmid

    #116724

    Purpose

    Lentiviral expression of CDK4

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGWB451

    Plasmid

    #74833

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3_cAMPFIRE-L

    Plasmid

    #182279

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L (cAMP sensor)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-Beta1

    Plasmid

    #140987

    Purpose

    Encodes a Gbeta subunit (GNB1) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GBeta1 (GNB1 Human)
    

    Expression

    Mammalian

    Mutation

    Contains an N-terminal human rhinovirus (HRV) 3C …

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. PEA1-GFP

    Plasmid

    #171993

    Purpose

    Delivers all prime editing nickase components in a single, GFP selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Expression

    Mammalian

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-(BbsI)_CBh-Cas9-T2A-BFP

    Plasmid

    #64323

    Purpose

    Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-EBFP2

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro

    Plasmid

    #73037

    Purpose

    Express Nluc and copGFP dual reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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