We narrowed to 7,454 results for: Lif

1. pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

   Plasmid

   #185678

   Purpose

   Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERmembrane-HaloTag-KKMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pH7m34GW-35S:SNACS S785A

   Plasmid

   #203577

   Purpose

   Plant SnRK2S30A kinase activity FRET sensor in plant expression vector

   Depositor

   Julian Schroeder

   Article

   Zhang et al eLife. 2020 May 28;9:e56351. doi: 10.755

   Insert

   SNACS S785A
   

   Use

   Tags

   StrepII

   Expression

   Plant

   Mutation

   S785A

   Promoter

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLVX-RT002-LV

   Plasmid

   #163382

   Purpose

   2nd generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

   Depositor

   Monte Winslow

   Article

   Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

   Insert

   LaG17-VEGFRtm (LV)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Halo-MDC1

   Plasmid

   #207108

   Purpose

   pHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 in human cells.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag-MDC1
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMA-SpCas9-g5

   Plasmid

   #80788

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 15x-QUAS-dTomato-T2A-GCaMP6s

   Plasmid

   #130666

   Purpose

   QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A

   Depositor

   Leslie Vosshall

   Article

   Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

   Insert

   15xQUAS-dTomato-T2A-GCaMP6s
   

   Use

   Ae. aegypti expression

   Tags

   Expression

   Insect

   Mutation

   Promoter

   15xQUAS-TATA

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMA-SpCas9-g6

   Plasmid

   #80789

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RNF168-Halo HRD

   Plasmid

   #207080

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   None

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-hChR2(H134R)-EYFP

   Plasmid

   #114367

   Purpose

   expresses channelrhodopsin 2 fused with EYFP

   Depositor

   Mingshan Xue

   Article

   Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

   Insert

   hChR2(H134R)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   the Histidine at amino acid position 134 was chan…

   Promoter

   pCAG

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MCP-ADAR2-DD

    Plasmid

    #170124

    Purpose

    Lentiviral vector carrying the MCP-ADAR2 with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MCP-ADAR2-DD-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a core promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. OSCA1.8

    Plasmid

    #136594

    Purpose

    Expresses the mechanosensitive channel OSCA1.8 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

    Insert

    OSCA1.8 (AT1G32090 Mustard Weed)
    

    Use

    Tags

    pIRES2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLVX-RT006-GCN4-HaloPM

    Plasmid

    #163386

    Purpose

    To express 4X sGCN4 module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    4XGCN4-Halotag-PDGFRtm-2A-mCherry (GCN4-HaloPM)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJCC_039 FnCas12a

    Plasmid

    #171666

    Purpose

    For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    FnCas12a
    

    Use

    Tags

    6X His and sortase A sorting motif

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-TRE-DIO-GCaMP6s

    Plasmid

    #166606

    Purpose

    Encodes Cre-dependent GCaMP6s under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST_6xUAS:miRFP670-cetn4

    Plasmid

    #192357

    Purpose

    zfCETN4 tagged with miRFP670, expressed under the UAS promoter

    Depositor

    Darren Gilmour

    Article

    Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

    Insert

    CETN4 (cetn4 Zebrafish)
    

    Use

    Tags

    miRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR R4 ATCC LacZ GCTT

    Plasmid

    #173668

    Purpose

    Destination vector for Golden Gate Cloning assemblies, blue-white screening

    Depositor

    Eric Marois

    Article

    Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMS84 (U6p::GGACAGTCCTGCCGAGGTGG)

    Plasmid

    #193852

    Purpose

    Encodes the guide RNA targeting the sequence GGACAGTCCTGCCGAGGTGG

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    Guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2-mCherry_SNAP_SNAPV_PACT_Active

    Plasmid

    #173072

    Purpose

    Active SNAP tag at the centrosome

    Depositor

    Heidi Hehnly

    Article

    Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

    Insert

    mCherry-SNAP-SNAP-Varied-PACT
    

    Use

    Tags

    mCherry-SNAP-SNAP-Varied

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. nontarget S. pyogenes scaffold CARGO array

    Plasmid

    #191319

    Purpose

    Contains an array of nontargeting guide RNAs

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Insert

    Array of guide RNAs
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEF HaloTag-SYB2

    Plasmid

    #197282

    Purpose

    Encodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYB2 and HaloTag (Vamp2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q76V and F77W

    Promoter

    EF1alpha

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. OSCA1.1

    Plasmid

    #136592

    Purpose

    Expresses the mechanosensitive channel OSCA1.1 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

    Insert

    OSCA1.1 (AT4G04340 Mustard Weed)
    

    Use

    Tags

    pIRES2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pWaldo-GFPe-SH1446

    Plasmid

    #112507

    Purpose

    Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.

    Depositor

    Simon Newstead

    Article

    Minhas et al Elife. 2018 Jul 3;7. pii: 34995. doi: 10

    Insert

    POT family transporter
    

    Use

    Tags

    C terminal TEV GFP octa-His affinity tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-Flp-DOG

    Plasmid

    #80084

    Purpose

    Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Insert

    Flp-DOG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. epB_UbC_tdMS2cp-tagRFP-Tx2

    Plasmid

    #119908

    Purpose

    ePiggyBac mammalian expression plasmid for tandem dimer MS2cp-tagRFP-T fusion

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    HA-tdMS2cp-tagRFP-Tx2-nls
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pME-PLIN2-tdTOMATO

    Plasmid

    #172533

    Purpose

    PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

    Depositor

    Richard White

    Article

    Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

    Insert

    plin2-tdtomato
    

    Use

    Tags

    Expression

    Mutation

    S80R, A81S, E144D, I148F, and R415H mutations in …

    Promoter

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GFP-FAK W266A

    Plasmid

    #186149

    Purpose

    For expressing FAK in mammalian cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBMN-dGBP1-TagBFP

    Plasmid

    #80087

    Purpose

    MMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection marker

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Insert

    dGBP1-TagBFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET11a-CA(A14C, E45C)

    Plasmid

    #79045

    Purpose

    Bacterial vector for cloning and propagation in DH5alpha

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    CA (A14C, E45C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A14C and E45C mutations in HIV-1 CA protein

    Promoter

    T7

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. U6c

    Plasmid

    #117223

    Purpose

    Expresses gRNA in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

    Insert

    HR U6c promoter-gRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. P305-FBP_4

    Plasmid

    #139695

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:tAtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge