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  13. pBRα-β flap (831-1057)

    Plasmid
    #53734
    Purpose
    Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    alpha NTD-Beta flap fusion
    Expression
    Bacterial
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available Since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    53734-plasmid-map-sequence-id-83920

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  22. p1D4-hrGFP II

    Plasmid
    #71317
    Purpose
    expression vector with 1D4 epitope added as a C-terminal tag, allowing for in vitro expression of any visual pigment or GPCR of interest.
    Depositor
    Belinda Chang
    Article
    Morrow et al Plasmid. 2010 Nov;64(3):162-9. doi: 10.1
    Type
    Empty backbone
    Tags
    1D4 epitope (TETSQVAPA) and IRES-hrGFPII
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  42. pYPQ290

    Plasmid
    #129670
    Purpose
    Gateway entry clone for CRISPR-Cas12b systems
    Depositor
    Yiping Qi
    Article
    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:
    Insert
    AacCas12b
    Use
    CRISPR; Gateway compatible aaccas12b entry clone
    Expression
    Plant
    Mutation
    AacCas12b is rice codon optimized
    Available Since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    129670-plasmid-map-sequence-id-255066

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Showing: 2101 - 2150 of 79968 results