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We narrowed to 5,270 results for: pcDNA3.1+

Showing: 2151 - 2200 of 5270 results

  10. pcDNA3.1-MS2-HA-HshnRNPH1_185-449-del291-361_Z

    Plasmid
    #148123
    Purpose
    Mammalian Expression of HshnRNPH1_185-449-del291-361
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPH1_185-449-del291-361 (HNRNPH1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148123-plasmid-map-sequence-id-290570

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  11. pcDNA3.1(+)-Myr-DnaJB1(H33Q)-PKAcat-mCherry

    Plasmid
    #181855
    Purpose
    mCherry-tagged DnaJB1-PKA catalytic subunit fusion N-terminal myristoylation site and H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.
    Depositor
    Jin Zhang
    Article
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Insert
    Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Histidine 33 in DnaJB1 changed to glutamine.
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181855-plasmid-map-sequence-id-362798

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  34. pcDNA3.1-N-Myc_DED/AAA 535-888 USP7

    Plasmid
    #131255
    Purpose
    Mammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    A mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131255-plasmid-map-sequence-id-267709

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  35. pcDNA3.1-CMV-LMO2 (GLuc-VChR1-EYFP)

    Plasmid
    #72889
    Purpose
    fusion protein of Gaussia luciferase, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO2) for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371
    Inserts
    Gaussia luciferase, Volvox channelrhodopsin 1, EYFP
    Volvox channelrhodopsin 1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    72889-plasmid-map-sequence-id-252489

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  46. pcDNA3.1 Flag YFP hNKCC1 V729C (NT503)

    Plasmid
    #50883
    Purpose
    Expresses human NKCC1 V729C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    V729C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    50883-plasmid-map-sequence-id-76356

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  47. pcDNA3.1 Flag YFP hNKCC1 Y739C (NT525)

    Plasmid
    #50893
    Purpose
    Expresses human NKCC1 Y739C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    Y739C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    50893-plasmid-map-sequence-id-76366

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  48. pcDNA3.1 Flag YFP hNKCC1 C723S (NT537)

    Plasmid
    #50902
    Purpose
    Expresses human NKCC1 C723S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    C723S in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50902-plasmid-map-sequence-id-76375

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  49. pcDNA3.1 Flag YFP hNKCC1 I674C (NT475)

    Plasmid
    #50875
    Purpose
    Expresses human NKCC1 I674C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I674C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50875-plasmid-map-sequence-id-76348

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  50. pcDNA3.1 Flag YFP hNKCC1 V740C (NT526)

    Plasmid
    #50894
    Purpose
    Expresses human NKCC1 V740C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    V740C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50894-plasmid-map-sequence-id-76367

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Showing: 2151 - 2200 of 5270 results