We narrowed to 13,646 results for: sequence
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Plasmid#48121PurposeShuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoterDepositorInsertcodon optimized signal peptide of YngK
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
attB53_SNCB+_attB53
Plasmid#183614PurposeRMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.DepositorInsertsLaG17 GFP nanobody SynNotch tTA
TRE-mCherry-SV40pA
tagBFP
UseRmceTagsMyc and human CD8a signal sequenceExpressionMammalianAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC6A2
Plasmid#131891PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC6A2 (SLC6A2 Human)
ExpressionMammalianAvailable SinceOct. 9, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUAST mito roGFP2-Grx1
Plasmid#64995Purposefor expression of mitochondrial roGFP2-Grx1 with the Gal4 expression system in drosophilaDepositorInsertGlutaredoxin-1 (GLRX Human)
TagsroGFP2ExpressionInsectMutationmitochondrial targeting sequenceAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR4 mito roGFP2-Grx1
Plasmid#65000PurposeExpression of mitochondrial roGFP2-Grx1 under the alphaTub84B promoterDepositorInsertGlutaredoxin-1 (GLRX Human)
TagsroGFP2ExpressionInsectMutationmitochondrial targeting sequenceAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-AktAR2
Plasmid#175013PurposePlasma membrane-targeted AktAR2.DepositorInsertLyn-mCherry-Akt-STOPS
TagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianPromoterCMVAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATOH1_pUC18
Plasmid#191250PurposeEncodes the sequence for the ATOH1 gene (human)DepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;lmo2CE1-P1Egfp
Plasmid#90153Purposecontains lmo2 enhancer sequence, and lmo2 specific gene promoter driving expression of eGFPDepositorAvailable SinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-eYFP-f
Plasmid#104057PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP with a C-terminal Hras farnesylation sequenceDepositorInsertEYFP-f
UseAAVPromoterTREAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4CE1-P1Egfp
Plasmid#90146Purposecontains dll4 enhancer 1 sequence, and dll4 specific gene promoter driving expression of eGFPDepositorAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-com-Ds
Plasmid#119077PurposeTo clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loopsDepositorInsertU6a:2xCom-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-MS2-Ds
Plasmid#119076PurposeTo clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loopsDepositorInsertU6a:2xMS2-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-AKARev(T/A)
Plasmid#118479PurposeNegative-control mutant for RAB-AKARev biosensor.DepositorInsertRAB-AKARev(T/A)
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RapR-FAK-YM-Y397F
Plasmid#25930DepositorInsertRapR-FAK (Ptk2 Human, Mouse)
TagsEGFPExpressionMammalianMutationThis pEGFP-RapR-FAK-YM-Y397F plasmid contains the…Available SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
p1.1-eGFP
Plasmid#162738PurposeFluorescent reporter for CHO cells expression studiesDepositorInsertenhanced green fluorescent protein
ExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available SinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
RhopH3-bio
Plasmid#47781PurposeExpresses enzymatically monobiotinylated full-length RhopH3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised RhopH3
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCYC1pr-GNSI (LEU)
Plasmid#111450PurposeGNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
TagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …Available SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-gp120(BaL)-CD4
Plasmid#98957PurposeMammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolateDepositorInsertHIV-1 (BaL) gp120
TagsCD4 (domains D1-D2), CD5 leader sequence, FLAG ep…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
p199 Dync1i2.C (Ex1a)
Plasmid#26449DepositorInsertcytoplasmic dynein 1 intermediate chain 2 isoform C (exon 1a) (Dync1i2 Mouse)
UseSubcloning and sequencingMutationN/AAvailable SinceMarch 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-POLArISact
Plasmid#164970PurposeT7 promotor drives in vitro transcription of POLArISact with a human kozak sequenceDepositorInsertPOLArISact
UseIn vitro transcriptionPromoterT7Available SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only