162,326 results

1. pCI-EGFP-NR2b wt

   Plasmid

   #45447

   Depositor

   Robert Malinow, Andres Barria

   Article

   Barria et al Neuron. 2002 Jul 18;35(2):345-53.

   Insert

   NR2B (Grin2b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   EGFP inserted after the predicted signal peptide …

   Promoter

   CMV

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF1983

   Plasmid

   #143562

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TBX5 (TBX5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUBC_HA_2xPP7-VP64

   Plasmid

   #68418

   Purpose

   Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   PP7(deltaFG)
   

   Use

   Synthetic Biology

   Tags

   HA and VP64

   Expression

   Mammalian

   Mutation

   PP7: [delta]67_74 (non-aggregating)

   Promoter

   human UbC

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB CAG-mTagBFP2-T2A-iCreERT2

   Plasmid

   #177308

   Purpose

   CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLTS

   Plasmid

   #59386

   Purpose

   Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Type

   Empty backbone

   Use

   Genome engineering

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGE651

   Plasmid

   #153229

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

   Plasmid

   #74010

   Purpose

   Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

   Depositor

   Filippo Del Bene

   Article

   Di Donato et al Genome Res. 2016 Mar 8.

   Inserts

   5xUAS
   Cas9
   Cre
   U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMV306hsp+LuxG13

   Plasmid

   #26161

   Depositor

   Brian Robertson, Siouxsie Wiles

   Article

   Andreu et al PLoS One. 2010 May 24;5(5):e10777.

   Insert

   Bacterial luciferase operon + G13 promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   It contains a gram-positive enhanced translation …

   Promoter

   Available since

   Feb. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-LC3-YFP

   Plasmid

   #99571

   Purpose

   Lentiviral expression of YFP-tagged LC3

   Depositor

   Edward Campbell

   Article

   Flavin et al Acta Neuropathol. 2017 May 19. doi: 10.1

   Insert

   LC3 (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3E-TurboID

    Plasmid

    #166570

    Purpose

    Multisite Gateway 3' entry clone for fusions onto the N-terminus of TurboID. Parton lab clone JSS

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    TurboID
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. A*66:01:01

    Plasmid

    #203291

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*66:01:01 (HLA-A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19/PLIN2 GFP-P2A-BFP

    Plasmid

    #202195

    Purpose

    knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

    Depositor

    James Olzmann

    Article

    Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

    Insert

    PLIN2 (PLIN2 Human)
    

    Use

    Cloning vector

    Tags

    GFP-P2A-BFP knock-in

    Expression

    Mutation

    Promoter

    lac

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. MiniCoopR U6:gRNA spred1, mitfa:Cas9

    Plasmid

    #118843

    Purpose

    Targets spred1 and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    spred1 gRNA (spred1 Zebrafish)
    

    Use

    CRISPR; Tol2

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-KRasCT

    Plasmid

    #99017

    Purpose

    Membrane marker

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

    Insert

    mCherry KRas CT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMRX-IP-mGFP-TAX1BP1

    Plasmid

    #192803

    Purpose

    Stable expression of mGFP-TAX1BP1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    NCOA4 (NCOA4 Human)
    

    Use

    Tags

    monomeric EGFP (A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. MMLVgag-3NES-Cre

    Plasmid

    #207252

    Purpose

    Generation of CreVLPs when used in conjunction with MMLV gag-pol during production

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    MMLVgag-3NES-Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5-HA-Ubiquitin-K11

    Plasmid

    #22901

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K11 only, all other lysines mutated to arginines

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAD/HisB-iRFP702

    Plasmid

    #45455

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP702
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T2A, S45T, D124E, M170L, D202L, I203V, I219V, R23…

    Promoter

    araBAD

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBFC0984

    Plasmid

    #231992

    Purpose

    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site

    Depositor

    Brady Cress

    Article

    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

    Inserts

    dRfxCas13d
    crRNA with 2xBsaI spacer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 and pTet

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTiger

    Plasmid

    #1728

    Purpose

    This is a 3rd generation lentiviral transfer vector (highest titer) from Nolan lab's FELIX vector system

    Depositor

    Garry Nolan

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge