4,168 results

1. MIGR1

   Plasmid

   #27490

   Depositor

   Warren Pear

   Article

   Pear et al Blood. 1998 Nov 15. 92(10):3780-92.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   GFP and IRES

   Expression

   Mammalian

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUGW-Pol2-ffLuc2-eGFP

   Plasmid

   #71394

   Purpose

   Lentiviral vector of luciferase-eGFP fusion gene driven by Pol2 promoter

   Depositor

   Glenn Merlino

   Article

   Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

   Insert

   Pol2-ffLuc2-eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Pol2 (mouse RNA polymerase II gene promtoer)

   Available Since

   Dec. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVUT-tTR-KRAB

   Plasmid

   #11651

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Has Service

   Cloning Grade DNA

   Insert

   hUbiquitin C, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPAmCherry1-N1

   Plasmid

   #31928

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

   Insert

   PAmCherry1
   

   Expression

   Mammalian

   Mutation

   E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

   Promoter

   cmv

   Available Since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 RLUC POLIRES FLUC

   Plasmid

   #45642

   Purpose

   Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

   Depositor

   Nahum Sonenberg

   Article

   Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

   Insert

   RLUC - IRES - FLUC
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-myc3-HDM2

   Plasmid

   #20935

   Depositor

   Yue Xiong

   Article

   Zhang et al Mol Cell Biol. 2003 Dec . 23(23):8902-12

   Insert

   Mdm2 p53 binding protein homolog (MDM2 Human)
   

   Tags

   myc3

   Expression

   Mammalian

   Available Since

   May 7, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV CEPIA2mt

   Plasmid

   #58218

   Purpose

   Green fluorescent indicator for calcium imaging in the mitochondria

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   CEPIA2mt
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GCaMP2 N263Y E282V M339L

   Promoter

   CMV

   Available Since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. sfGFP-C1

   Plasmid

   #54579

   Purpose

   Localization: C1 Cloning Vector, Excitation: 485, Emission: 507

   Depositor

   Geoffrey Waldo, Michael Davidson

   Article

   Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

   Type

   Empty backbone

   Tags

   sfGFP

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCDNA-3xHA-hTERT

   Plasmid

   #51637

   Purpose

   Mammalian expression of 3xHA tagged human TERT, N-terminal.

   Depositor

   Steven Artandi

   Article

   Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

   Insert

   TERT (TERT Human)
   

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. Laconic/pcDNA3.1(-)

    Plasmid

    #44238

    Purpose

    a genetically-encoded sensor for lactate concentration, transport and metabolic flux

    Depositor

    Luis Felipe Barros

    Article

    San Martin et al PLoS One. 2013;8(2):e57712. doi: 10.1371

    Insert

    Laconic (LACtate Optical Nano Indicator from CECs)
    

    Use

    Lactate biosensor

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cbh_v5 AAV-CBE N-terminal

    Plasmid

    #137175

    Purpose

    AAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-CBE N-terminal; U6-protospacer
    

    Use

    AAV

    Mutation

    Cas9 D10A

    Promoter

    Cbh

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. T7-VEE-OKS-iM

    Plasmid

    #58972

    Purpose

    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Inserts

    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Promoter

    26S subgenomic promoter and IRES

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-EF1-Luc2-P2A-tdTomato

    Plasmid

    #72486

    Purpose

    Express Luc2 and tdTomato under EF1 promoter

    Depositor

    Kazuhiro Oka

    Insert

    Luc2-P2A-tdTomato
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-dCas13-M3nls

    Plasmid

    #155366

    Purpose

    Targeted m6A RNA methylation in mammalian cells

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRIP-GFP-IRF3

    Plasmid

    #127663

    Purpose

    Over-express EGFP-IRF3

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    IRF3 (IRF3 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. v3em-Nterm-PE2max

    Plasmid

    #198734

    Purpose

    AAV genome encoding N-terminal PE2max

    Depositor

    David Liu

    Article

    Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

    Insert

    Nterm PE2max-NpuN
    

    Use

    AAV

    Promoter

    Cbh

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-E-cadherin-RFP

    Plasmid

    #79603

    Purpose

    pHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging system

    Depositor

    Jeffrey Rosen

    Article

    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

    Insert

    CDH1 promoter region (CDH1 Human)
    

    Use

    Lentiviral

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA6a-TurboID-3MYC-kanMX6 (pFB1420)

    Plasmid

    #126049

    Purpose

    For C-terminal tagging with TurboID in yeast including geneticin-resistant marker

    Depositor

    François Bachand

    Article

    Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

    Insert

    TurboID
    

    Use

    Pcr-based c-terminal tagging

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3/F-tractin=mStayGold

    Plasmid

    #212019

    Purpose

    For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    F-tractin
    

    Tags

    mStayGold

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDG02583

    Plasmid

    #104129

    Purpose

    Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    NEDD8-specific protease 1 (NEDP1)
    

    Tags

    14x Histidine tag, Maltose binding protein, and S…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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