We narrowed to 7,692 results for: Lif;
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Plasmid#127907PurposeWT Cas9 Vector targeting the 3' end of the human HP1a geneDepositorInsertgRNA for Human 3' HP1a
UseCRISPRAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SEO-CaMKII-TVA-P2A-N2cG
Plasmid#172363PurposeTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from excitatory cortical neurons which do not express cre-recombinaseDepositorInsertTVA + B19-N2cG
UseAAV and Cre/LoxExpressionMammalianPromoterCaMKIIaAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mCherry-mTrim21ΔPRYSPRY
Plasmid#105524PurposeT7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted PRYSPRY domain mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmCherryMutationdeletion of PRYSPRY domain (aa1-281)PromoterT7Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_His-ZZ-TEV-Lis1
Plasmid#132539PurposePlasmid for insect cell expression of human full-length LIS1 with an N-terminal His-ZZ-LTLT tagDepositorAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST_5xUAS:EB3-mScarlet-I
Plasmid#192358PurposeEB3 tagged with mScarlet-I, expressed under the UAS promoterDepositorAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-BclXL-pEGFP-C1
Plasmid#177408PurposeTo express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.DepositorInsertBcl-XL
TagsmCerulean3ExpressionMammalianPromoterCMVAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1-HLx2
Plasmid#108133PurposeRecruitable SAC1 with extended linkerDepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG::SACM1L(1-520):[EAAAR]2:SACM1L(521-587) (SACM1L Human)
ExpressionMammalianMutationSACM1L(1-520):[EAAAR]2:SACM1L(521-587)PromoterCMVAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-SA-neo-CAGGS-PE2-2A-GFP
Plasmid#180014PurposeTargeting vector for knocking in constitutively expressed PE2-2A-GFP into the human AAVS1 locusDepositorInsertPE2-2A-GFP
UseCRISPR; Human genome engineeringTagsGFPPromoterCAGAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-AP-Smo-YFP-DEST
Plasmid#49099PurposeExpressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell linesDepositorAvailable SinceNov. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-CB5
Plasmid#171007PurposeHiLITR protease with CB5 C-terminal tmd targeting information (ER)DepositorInsertEGFP-uTEV1-CB5(tmd)
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterpTRE-tight (TetOn)Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-DNAJC5
Plasmid#205727PurposeMammalian expression of DNAJC5DepositorInsertDNAJC5 (DNAJC5 Human)
UseLentiviralAvailable SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fireworks GFP[PTC-] (AVA2598)
Plasmid#85445PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
UseMinimal backbone fragment for low-copy bacterial …ExpressionMammalianAvailable SinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ROS1-V5/HIS
Plasmid#205609PurposeExpression of human ROS1 receptor tyrosine kinase in mammalian cellsDepositorAvailable SinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
p130Cas Y15F
Plasmid#186154PurposeFor expressing recombinant p130Cas in insect cells.DepositorInsertBCAR1 (Bcar1 Rat)
TagsHis6 (Tev)ExpressionInsectMutation15Y residues mutated to AlaninePromoterpolyhedrinAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
PP023 - CAG::QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX
Plasmid#182063PurposeCo-expression of Arch-based voltage indicator (QuasAr6a) and membrane-localized calcium reporter (jRGECO1a-CAAX)DepositorInsertQuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX
UseLentiviralPromoterCAGAvailable SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Fireworks RFP[PTC-] (AVA2515)
Plasmid#85443PurposeExpresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-HygromycinR
UseMinimal backbone fragment for low-copy bacterial …ExpressionMammalianAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-Turbo-NES-YFP-STOP-L2
Plasmid#127358Purposegateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choiceDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-Nrxn1α-LNS3
Plasmid#128107PurposeExpress HA tagged Nrxn1α-LNS3DepositorAvailable SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-Nrxn1α-LNS1
Plasmid#128105PurposeExpress HA tagged Nrxn1α-LNS1DepositorAvailable SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ mWnt5a
Plasmid#102860PurposeExpresses mouse Wnt5a in mammalian cells, zebrafish or XenopusDepositorInsertMouse Wnt5a
UseZebrafish expression; xenopus expressionExpressionMammalianPromoterCMV; SP6Available SinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2
Plasmid#166972PurposeExpresses FBF-2 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
TagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575PromoterT7Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-p62-LIR-mutant (LIRAAAA)
Plasmid#187981PurposePlasmid for the expression of a p62 mutant (LIR mutant: p62 3xDW + silent mutation). Internal reference: SMC528DepositorInsertp62 (NUP62 Human)
TagsmCherryExpressionMammalianMutationDDDW335-338AAAA (LIR mutant), silent mutation siR…Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFE-Selo-FI-prk
Plasmid#162698PurposeExpresses S. elongatus Form IB rubisco and S. elongatus PrkDepositorInsertS. elongatus Form IB rubisco and S. elongatus Prk
TagsN terminal 6x His tag on PRKExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sema6c-AP-His
Plasmid#72041PurposeExpresses the extracellular region of the Sema6C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDSP16 (5’Δ Cbr-unc-119(+) + loxN + MCS + 5’Δ mNeonGreen)
Plasmid#193854PurposePromoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen landing padDepositorInsert5' Cbr-unc-119(+) + 5' mNeonGreen
UseCRISPR and Cre/LoxExpressionWormPromoterCbr-unc-119pAvailable SinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
mAMCase_CatD_6xHis_pTwistCMV
Plasmid#200228PurposeExpresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pTwist CMV vectorDepositorInsertAcidic Mammalian Chitinase (Chia1 Mouse)
Tags6xHisExpressionMammalianMutationS354FPromoterCMVAvailable SinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sema6d.4-AP-His
Plasmid#72044PurposeExpresses the extracellular region of the Sema6D, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3c(L)-AP-His
Plasmid#72015PurposeExpresses the Sema3C protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-LGALS1
Plasmid#175755PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS1DepositorInsertLGALS1 (LGALS1 Human)
UseLentiviralAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX317-hcRED
Plasmid#115440PurposeConstitutive lentiviral expressionDepositorInserthcRED
UseLentiviralTagsV5ExpressionMammalianPromoterEF1aAvailable SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Plxnb1(L)-Fc-His
Plasmid#72126PurposeExpresses the entire extracellular region of the PlexinB1 protein (ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TIE-V5/HIS
Plasmid#205612PurposeExpression of human TIE receptor tyrosine kinase in mammalian cellsDepositorAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 AMSH 1-178
Plasmid#160050PurposeExpresses MIT domain (residues 1-178) of AMSH as a HIS-SUMO fusion protein. Internal ID: WISP18-22.DepositorInsertAMSH residues 1-178 (STAMBP Human)
TagsHIS-SUMOExpressionBacterialMutationResidues 1-178PromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-mCherry-SPASTIN
Plasmid#180647PurposeLentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.DepositorInsertSPASTIN
UseLentiviralTagsmCherryExpressionMammalianMutationStarts at M87, has siRNA resistance to GAACAGUGUG…Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-CALCOCO2
Plasmid#175759PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-CALCOCO2/NDP52DepositorInsertNDP52/CALCOCO2 (CALCOCO2 Human)
UseLentiviralAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mCherry-mTrim21ΔRING-Box
Plasmid#105523PurposeT7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmCherryMutationdeletion of RING domain (aa124-462)PromoterT7Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-nl.mCherry-GCaMP7s
Plasmid#172385PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized mCherry + GCaMP7s
UseNeurotropic virusAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sema3e(L)-AP-His
Plasmid#72019PurposeExpresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-NES
Plasmid#171006PurposeHiLITR protease with C-terminal NNES (Cytoplasmic)DepositorInsertEGFP-uTEV1-NES
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterpTRE-tight (TetOn)Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCCM
Plasmid#162708PurposeSecond CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM clusterDepositorInsertSecond CCM operon cloned from H. neapolitanus
ExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
UAS-u(S)Cas9
Plasmid#127383PurposeExpresses Cas9 at high levelDepositorInsertuORF(S)-Cas9
UseCRISPRExpressionInsectPromoterUASTAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-miniTurbo-NES-YFP-STOP-L2
Plasmid#127359Purposegateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choiceDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFE-Rrub-FII-prk
Plasmid#162695PurposeExpresses R. rubrum Form II rubisco and S. elongatus PrkDepositorInsertsRubrum Form II rubisco
S. elongatus PRK
ExpressionBacterialPromoterPLtet0-1 promoter and under same promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sema4g-Fc-His
Plasmid#72160PurposeExpresses the extracellular region of the Sema4G protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-tdTomato-iCre
Plasmid#172374PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInserttdTomato + iCre
UseNeurotropic virusAvailable SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-RNF68
Plasmid#126545PurposeExpresses mCherry-tagged RNF68 in mammalian cellsDepositorAvailable SinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sema3g.a(L)-Fc-His
Plasmid#72151PurposeExpresses the Sema3G, isoform a protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
SnFR-gamma2
Plasmid#165495PurposeGreen fluorescent glutamate reporter fused to TARP gamma-2 for mammalian expression.DepositorInsertiGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Synthetic, Rat)
ExpressionMammalianPromoterCMVAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-RNF68
Plasmid#126548PurposeExpresses FLAG-HA-tagged RNF68 in mammalian cellsDepositorAvailable SinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-MSI1(FL)
Plasmid#60358Purposeexpress mouse MSI1 in mammalian cellsDepositorAvailable SinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only