We narrowed to 6,417 results for: nls

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pLVX-ATF4 mScarlet NLS

Plasmid

#115969

Purpose

ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

Depositor

David Andrews

Article

Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

Insert

activating transcription factor 4 (ATF4 Human, Synthetic)

Use

Lentiviral

Tags

HA tag, c-myc NLS, and mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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LentiGuide-puro-NLS-mCherry

Plasmid

#185474

Purpose

Mammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Type

Empty backbone

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-AsCas12f-2NLS

Plasmid

#171613

Purpose

NLS-AsCas12f1-NLS heterologous expression plasmid for RNP

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

NLS-AsCas12f1-NLS

Expression

Bacterial

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDN34-BiNLS-hYAP1-1delta

Plasmid

#169471

Purpose

expresses optoYAP in mammalian cells

Depositor

Timothy Saunders

Article

Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

Insert

YAP1-1delta (YAP1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pUBC-SV40NLS-Pfv-Sapphire

Plasmid

#203653

Purpose

Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

Lentiviral

Tags

Sapphire

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pGG-GFP-TurboID-3XNLS

Plasmid

#209400

Purpose

Transiently expressing and visualizating the subcellular localization of TurboID fused with 3X nuclear localization signal (NLS) in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

3XHA-TurboID-3XNLS

Tags

GFP

Expression

Plant

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGs-NLS-TIR1_P2A_NES-TIR1

Plasmid

#117699

Purpose

Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Inserts

TRANSPORT INHIBITOR RESPONSE 1
TRANSPORT INHIBITOR RESPONSE 1

Tags

HA tag, Myc-NES, and NLS

Expression

Mammalian

Mutation

codon optimized for murine expression

Promoter

None, 2A peptide from porcine teschovirus-1 polyp…

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pET-Cas9-NLS-6xHis

Plasmid

#62933

Purpose

Expression of Cas9-NLS-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

Cas9-NLS-6xHis

Tags

6xHis tag and NLS

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pDY0750 pCAG-NLS-BceINT

Plasmid

#179112

Purpose

BceINT expression vector

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCAG-NLS-BceINT

Use

CRISPR

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET-NLS-Cas9-6xHis

Plasmid

#62934

Purpose

Expression of NLS-Cas9-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

NLS-Cas9-6xHis

Tags

6xHis tag and NLS

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pUbC-nls-ha-stdMCP-stdGFP

Plasmid

#98916

Purpose

synonymized tandem dimer MCP fused to synonymized tandem dimer GFP

Depositor

Robert Singer

Article

Wu et al Genes Dev. 2015 Apr 15;29(8):876-86. doi

Insert

stdMCP-stdGFP

Use

Lentiviral

Tags

GFP

Promoter

UbC

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-NLS-FRB-VP16

Plasmid

#210515

Purpose

expresses NLS-FRB-VP16 component in mammalian cells

Depositor

Dongmin Lee

Article

Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

Insert

NLS-FRB-VP16

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Utr230-EGFP-3XNLS

Plasmid

#58466

Purpose

Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

Utr230 with 3X glycine linker (UTRN Human)
3X NLS with 3X stop codons

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-MBP-2xNLS-tdTomato

Plasmid

#104054

Purpose

An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-tdTomato

Use

AAV

Tags

NLS

Promoter

MBP

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EFS_mCherry2-NLS-PEST_Puro

Plasmid

#213775

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

EFS_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-mClover3-TDP-43ΔNLS

Plasmid

#214917

Purpose

inducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear puncta

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

TDP-43ΔNLS (TARDBP Human)

Use

Lentiviral

Tags

mClover3

Mutation

mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-mDlx-NLS-mRuby2

Plasmid

#99130

Purpose

An AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

NLS-mRuby2

Use

AAV

Expression

Mammalian

Promoter

mDLX5/6

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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R4pGWB601_UBQ10p-miniTurbo-YFP-NLS

Plasmid

#127370

Purpose

Binary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plants

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Tags

GS linker, NLS, V5, and YFP

Expression

Plant

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

Arabidopsis UBQ10 promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-NLS

Plasmid

#86677

Purpose

Expresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

EGFP-NLS

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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