We narrowed to 6,177 results for: nls

1. pUbC-nls-ha-stdMCP-stdGFP

   Plasmid

   #98916

   Purpose

   synonymized tandem dimer MCP fused to synonymized tandem dimer GFP

   Depositor

   Robert Singer

   Article

   Wu et al Genes Dev. 2015 Apr 15;29(8):876-86. doi

   Insert

   stdMCP-stdGFP
   

   Use

   Lentiviral

   Tags

   GFP

   Promoter

   UbC

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLL5.0 shCAV1-NLS-mCherry

   Plasmid

   #187257

   Purpose

   Lentiviral expression of shRNA targeting Caveolin1 and NLS-mCherry

   Depositor

   Alpha Yap

   Article

   Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

   Insert

   ShRNA for Caveolin-1 (Cav1 Mouse)
   

   Use

   Lentiviral

   Tags

   NLS-mCherry

   Promoter

   U6

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICE-RNaseHI-WT-NLS-mCherry

   Plasmid

   #60365

   Purpose

   Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

   Depositor

   Patrick Calsou, Sébastien Britton

   Article

   Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

   Insert

   RNase HI
   

   Tags

   NLS from SV40 T antigen and mCherry

   Expression

   Mammalian

   Promoter

   CMV-tet

   Available Since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1 Utr230-EGFP-3XNLS

   Plasmid

   #58466

   Purpose

   Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

   Inserts

   Utr230 with 3X glycine linker (UTRN Human)
   3X NLS with 3X stop codons
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET-eSpCas9-NLS-6xHis

   Plasmid

   #207379

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-NLS-HA-TurboID-PCNA

   Plasmid

   #215076

   Purpose

   Expresses NLS-HA-TurboID-PCNA in mammalian cells from a lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   PCNA (PCNA Human)
   

   Use

   Lentiviral

   Tags

   NLS-HA-TurboID

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PiggyBac-TDP-43deltaNLS-V5-APEX

   Plasmid

   #229081

   Purpose

   Doxycycline-inducible expression of NLS-mutated TDP-43 fused to V5-tag and APEX for proximity labeling, containing BFP in the backbone

   Depositor

   Eran Hornstein

   Insert

   TDP-43deltaNLS (TARDBP Human)
   

   Tags

   APEX, BFP, and V5

   Expression

   Mammalian

   Mutation

   K82A, R83A, K84A, K95A, K97A and R98A for mutatio…

   Promoter

   TRE3G

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGG-GFP-TurboID-3XNLS

   Plasmid

   #209400

   Purpose

   Transiently expressing and visualizating the subcellular localization of TurboID fused with 3X nuclear localization signal (NLS) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   3XHA-TurboID-3XNLS
   

   Tags

   GFP

   Expression

   Plant

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available Since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-FNLS-P2A-Puro

   Plasmid

   #110841

   Purpose

   Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-FNLS
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   D10A and NLS sequence at the N-terminus

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. R4pGWB601_UBQ10p-Turbo-YFP-NLS

    Plasmid

    #127368

    Purpose

    Binary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Plant

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Arabidopsis UBQ10 promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV2-CAG-3xNLS-vsfGFP-0

    Plasmid

    #191097

    Purpose

    To express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Pantelis Tsoulfas, Murray Blackmore

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-vsfGFP-0
    

    Use

    AAV

    Tags

    Green fluorescent protein bound to enhancer nanob…

    Expression

    Mammalian

    Mutation

    Optimized to Human codon usage

    Promoter

    CMV and CAG

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-His:dCas9:nls:10P3

    Plasmid

    #135982

    Purpose

    Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells

    Depositor

    Roman Jerala

    Article

    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

    Insert

    dCas9 fused to 10 copies of the P3 coiled-coil
    

    Use

    CRISPR

    Tags

    His

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTol-Promoter:NLS-tdMCP-eGFP

    Plasmid

    #86248

    Purpose

    For generating transgenic lines that ubiquitously expressing the fluorescent MCP reporter

    Depositor

    Florence Marlow

    Article

    Campbell et al Development. 2015 Apr 1;142(7):1368-74.

    Insert

    beta Actin promoter:NLS-tdMCPeGFP
    

    Use

    Used with transposase to make transient or stable…

    Tags

    NLS-tdMCP-eGFP

    Promoter

    Beta Actin

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. GW1-Peredox-mCherry-NLS

    Plasmid

    #32381

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    Peredox-mCherry-NLS
    

    Tags

    Nuclear Localization Seq

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDN34-BiNLS-hYAP1-1delta

    Plasmid

    #169471

    Purpose

    expresses optoYAP in mammalian cells

    Depositor

    Timothy Saunders

    Article

    Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

    Insert

    YAP1-1delta (YAP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLHCX-ATF4 mScarlet NLS

    Plasmid

    #115970

    Purpose

    ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I (retroviral vector backbone)

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    activating transcription factor 4 (ATF4 Synthetic, Human)
    

    Use

    Retroviral

    Tags

    HA tag, c-myc NLS, and mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-Peredox-mCherry-NLS

    Plasmid

    #32384

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    Peredox-mCherry-NLS
    

    Tags

    Nuclear Localization Seq

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPBpuro-hM3D-P2Av3-mCherryNLS

    Plasmid

    #184714

    Purpose

    Gq DREADD

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    hM3D
    

    Expression

    Mammalian

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHRm-GFP(1-10)-VP64-NLS

    Plasmid

    #70228

    Purpose

    Expresses GFP(1-10)-VP64-NLS in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP(1-10)-VP64-NLS
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDY0750 pCAG-NLS-BceINT

    Plasmid

    #179112

    Purpose

    BceINT expression vector

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCAG-NLS-BceINT
    

    Use

    CRISPR

    Available Since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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