We narrowed to 6,501 results for: nls

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pCMV-NLS-BioID2-HA

Plasmid

#232025

Purpose

mammalian expression (CMV promoter) of nuclear targeted BioID2-HA

Depositor

Primo Schaer

Publication

Richina et al Cell Mol Life Sci. 2025 Nov 25. doi: 10.

Insert

NLS

Tags

NLS

Expression

Mammalian

Available since

Nov. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pME-nls

Plasmid

#108882

Purpose

Multisite gateway vector for 5' tagging with an N-terminal nuclear localisation sequence

Depositor

Rob Parton

Publication

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

nls

Use

Gateway Cloning

Available since

Oct. 4, 2020

Availability

Academic Institutions and Nonprofits only

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NLS-mCherry

Plasmid

#221216

Purpose

control plasmid-part B of SPARK-OFF

Depositor

Xiaokun Shu

Publication

Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NLS-mCherry

Expression

Mammalian

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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FokI(inactive)-mCherry-LacI-NLS

Plasmid

#215100

Purpose

Expresses FokI(inactive)-mCherry-LacI-NLS in mammalian cells.

Depositor

Michele Pagano

Publication

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FokI(inactive)-mCherry-LacI-NLS

Expression

Mammalian

Mutation

FokI(inactive) mutant D69A.

Promoter

CMV

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pW266-lenti-spsgRNA-hsCDH1-sg2-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170815

Purpose

Lentiviral vector to co-express a human CDH1 spsgRNA (sg2-Cdh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Publication

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mScarlet-I-P2A-BlastR

Use

Lentiviral

Expression

Mammalian

Mutation

NA

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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S3_008_026_MV1

Plasmid

#134420

Purpose

pYTKMV1-pRPL18B_ NLS_FdeR_N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pRPL18B

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S3_009_026_MV1

Plasmid

#134421

Purpose

pYTKMV1-pREV1_ NLS_FdeR-N_2NLS-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pREV1

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S3_008_026_096

Plasmid

#134411

Purpose

pYTK096-pRPL18B_ NLS_FdeR_N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pRPL18B

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S3_009_026_096

Plasmid

#134412

Purpose

pYTK096-pREV1_ NLS_FdeR-N_2NLS-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pREV1

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

Plasmid

#121174

Purpose

Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines

Depositor

Timo Otonkoski

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro

Use

CRISPR

Expression

Mammalian

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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PABE-3

Plasmid

#115624

Purpose

Targeted A to G in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Publication

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

wtTadA-TadA7.10-NLS-nCas9-NLS

Use

CRISPR

Expression

Plant

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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PABE-4

Plasmid

#115625

Purpose

Targeted A to G in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Publication

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

NLS-nCas9-wtTadA-TadA7.10-NLS

Use

CRISPR

Expression

Plant

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

Plasmid

#191796

Purpose

vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

Depositor

Huawei Tong

Publication

Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

humanized dCas13X

Use

CRISPR

Expression

Mammalian

Mutation

R84A, H89A, R739A, R740A, H745A, H746A, H747A

Promoter

CBh, SV40, hU6

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pB–DualPE

Plasmid

#235161

Purpose

For plant prime editing including large DNA fragment editing in wheat plants or other monocotyledons

Depositor

Zong Yuan

Publication

Zhao et al Nat Plants. 2025 Feb;11(2):191-205. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Csy4-P2A-Nls-nCas9-NC-nls-MLV-Nls
CmYLCV-epegRNA-CaMV poly(A) signal

Use

CRISPR

Mutation

Detailed in manuscript

Available since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pRO204

Plasmid

#233843

Purpose

MoClo-YTK; ConL1/R2 cassette; ABA systems; pTDH3-NLS-VP48-ABI-tENO1

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-NLS-VP48-ABI-tENO1

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO279

Plasmid

#233845

Purpose

MoClo-YTK; ConL1/R2 cassette; ABA systems; pTDH3-NLS-VP48-1xOaf1-ABI-tENO1

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-NLS-VP48-1xOaf1ABI-tENO1

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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S3_007_026_MV1

Plasmid

#134419

Purpose

pYTKMV1-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S3_007_026_MV2

Plasmid

#134424

Purpose

pYTKMV2-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S3_007_026_096

Plasmid

#134410

Purpose

pYTK096-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pTDH3

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag

Plasmid

#132411

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dRfxCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R239A; H244A; R858A; H863A

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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