We narrowed to 6,639 results for: WUS;

Showing: 1801 - 1840 of 6639 results

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pBAD-R-GECO1.2

Plasmid

#113937

Purpose

Bacterial expression of red fluorescent Ca2+ sensor R-GECO1.2

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

R-GECO1.2

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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LZF70: Cre-off hSyn-QuasAr3-dark Citrine (FAS)

Plasmid

#104117

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

QuasAr3-dark Citrine

Use

Lentiviral

Expression

Mammalian

Promoter

human synapsin I

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLN342 (FuGW-SSX1p-mKate2)

Plasmid

#105172

Purpose

SSX1 promoter drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCSJ95

Plasmid

#104343

Purpose

Express yeast Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

Depositor

Alexander Varshavsky

Article

Chen et al Science. 2017 Jan 27;355(6323). pii: 355

Insert

FBP1

Tags

3xFLAG

Expression

Yeast

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLN343 (FuGW-H2A1p-mKate2 )

Plasmid

#105173

Purpose

H2A1 promoter drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

H2A1p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSF11-wmCardinal

Plasmid

#197251

Purpose

Expresses the protein of wmCardinal in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmCardinal

Expression

Worm

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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p63RhoGEF580-mVenus-N1

Plasmid

#84338

Purpose

Expresses p63 (580 a.a.) tagged with mVenus

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2016 Nov 11;6:36825. doi: 10.10

Insert

p63RhoGEF (580 aa) (ARHGEF25 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a-hAIM2-deltaPYD

Plasmid

#51522

Purpose

Express His6-tagged HIN domain of hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

His6 and T7

Expression

Bacterial

Mutation

deletion of pyrin domain

Promoter

lac operator

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mAIM2-PYD-EGFP

Plasmid

#51610

Purpose

Express GFP-tagged PYD of mAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

deletion of HIN domain

Promoter

MESV

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA p53, mitfa:Cas9

Plasmid

#118841

Purpose

Targets tp53 and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

tp53 gRNA (tp53 Zebrafish)

Use

CRISPR; Tol2

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pDualIN-SMN2

Plasmid

#214430

Purpose

Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

SMN2 EXON 7 (SMN2 Human)

Expression

Mammalian

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term SRSF1

Plasmid

#187575

Purpose

Express FLAG epitope and APEX2-tagged SRSF1 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

SRSF1 (SRSF1 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDA034

Plasmid

#131386

Purpose

ORFeus expression, mutant

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Tags

ORF2-3xFlag

Expression

Mammalian

Mutation

D702Y RT

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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dABE

Plasmid

#124447

Purpose

Expresses dead Cas9 (dCas9)-ABE7.10

Depositor

George Church

Article

Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

Insert

dABE7.10

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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dCBE4-gam

Plasmid

#124449

Purpose

Expresses dead Cas9 (dCas9)-CBE4-gam

Depositor

George Church

Article

Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

Insert

dCBE4-gam

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

D10A, H840A

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_SOX2_P2A_Hygro_Barcode

Plasmid

#120480

Purpose

Barcoded lentiviral vector to express SOX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SOX2 (SOX2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_CRX_P2A_Hygro_Barcode

Plasmid

#120433

Purpose

Barcoded lentiviral vector to express CRX in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

CRX (CRX Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3A-BE3-Y130F

Plasmid

#113428

Purpose

Expresses hA3A-BE3-Y130F in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-BE3-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_Y130F

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-mouse Ezh2 delta SETdomain-IRES-GFP

Plasmid

#91885

Purpose

Retroviral expression of mouse Ezh2 with a SET domain deletion

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Ezh2 (Ezh2 Mouse)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

SET domain deleted

Promoter

MMLV gag

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pSMVP-Cas9C

Plasmid

#80939

Purpose

Plasmid that expresses Cas9C in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-SOUL-P2A-TdTomato-WPRE-hGH-polyA

Plasmid

#177576

Purpose

Expresses SOUL in neuronal cell types

Depositor

Guoping Feng

Article

Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

Insert

SOUL

Use

AAV

Tags

tdTomato

Promoter

hSyn

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)

Plasmid

#169188

Purpose

To express His-SUMO-nsp12 (SARS-CoV-2) in E. coli

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

14His-SUMO-nsp12 (ORF1ab Synthetic, SARS-CoV-2)

Tags

14His-SUMO (Ulp1-cleavable)

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
DTA in pCDEST2

Plasmid

#223705

Purpose

Mammalian expression vector encoding diptheria toxin A

Depositor

Rob Parton

Article

Luong et al ACS Nano. 2025 Dec 23;19(50):42079-42096

Insert

Diphtheria toxin A

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCXLE-mCherry

Plasmid

#193293

Purpose

episomal vector expressing mCherry fluorescent protein

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-sgMyc_1

Plasmid

#138324

Purpose

Express guide RNA 2 for mouse Myc

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

cMyc sgRNA (Myc Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P2-p53 (1-90) (72R)

Plasmid

#62081

Purpose

Expression of human p53 (TAD residues 1-90) (72R) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains TAD/PP residues 1-90, 72R

Promoter

tac

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-TDAG51

Plasmid

#32699

Depositor

Richard C. Austin

Article

Hossain et al J Biol Chem. 2003 Aug 8;278(32):30317-27

Insert

human tdag51 (PHLDA1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
pdCas12b(RuvC)

Plasmid

#186450

Purpose

Encodes catalytically inactive Cas12b (E848A, D977A) from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

dCas12b

Use

CRISPR

Mutation

E848A, D977A

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
131_pAAV-ProB13-CatCh-GFP-WPRE

Plasmid

#125933

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProB13

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
27_pAAV-ProB12-CatCh-GFP-WPRE

Plasmid

#125932

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProB12

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
46_pAAV-ProC11-CatCh-GFP-WPRE

Plasmid

#125946

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC11

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
11_pAAV-ProC6-CatCh-GFP-WPRE

Plasmid

#125942

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC6

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
35_pAAV-ProC8-CatCh-GFP-WPRE

Plasmid

#125943

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC8

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
40_pAAV-ProC9-CatCh-GFP-WPRE

Plasmid

#125944

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC9

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
193_pAAV-ProA35-CatCh-GFP-WPRE

Plasmid

#125918

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProA35

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
140_pAAV-ProA33-CatCh-GFP-WPRE

Plasmid

#125916

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProA33

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
95_pAAV-ProA28-CatCh-GFP-WPRE

Plasmid

#125911

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProA28

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
61_pAAV-ProA18-CatCh-GFP-WPRE

Plasmid

#125901

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProA18

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
55_pAAV-ProA13-CatCh-GFP-WPRE

Plasmid

#125896

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProA13

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
5_pAAV-ProA9-CatCh-GFP-WPRE

Plasmid

#125893

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProA9

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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