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We narrowed to 7,986 results for: 104

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Showing: 2251 - 2300 of 7986 results

  11. ATP1A1_G8_Dual_sgRNA

    Plasmid
    #178104
    Purpose
    Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G8 sgRNA + user-specified sgRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178104-plasmid-map-sequence-id-350193

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  15. pLJM60 FLAG-RAB35 wt

    Plasmid
    #107104
    Purpose
    Lentiviral vector that codes for FLAG-RAB35 wildtype (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.
    Depositor
    Charles Sawyers
    Article
    Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi
    Insert
    RAB35 (RAB35 Human)
    Use
    Lentiviral
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    wildtype
    Promoter
    CMV
    Available Since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    107104-plasmid-map-sequence-id-205812

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  30. H52-pUCM-hNGN2-inv

    Plasmid
    #110492
    Purpose
    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm
    Depositor
    Michael Ward
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    mcherry
    rtTA3G
    Use
    CRISPR, Cre/Lox, and TALEN
    Expression
    Mammalian
    Promoter
    CAG, EF-1 alpha, and TRE3G
    Available Since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    110492-plasmid-map-sequence-id-207872

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  33. LifeAct-14 (LA-14)

    Plasmid
    #158750
    Purpose
    Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.
    Depositor
    Minhajuddin Sirajuddin
    Article
    Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152
    Insert
    LifeAct-14
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Deletion mutation for last three amino acids from…
    Promoter
    CMV immediate early
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158750-plasmid-map-sequence-id-310555

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  36. pTPH413

    Plasmid
    #185728
    Purpose
    Bacterial PAM profiling of adenine base editor variants
    Depositor
    David Liu
    Article
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Inserts
    ecTadA(8e)-neNme2-C
    Nme2Cas9 sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…
    Available Since
    Sept. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    185728-plasmid-map-sequence-id-364088

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  40. gNS2B47NS3 S135A

    Plasmid
    #155317
    Purpose
    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutant
    Depositor
    Dahai Luo
    Article
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    S135A
    Promoter
    M13
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155317-plasmid-map-sequence-id-311234

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  43. pLKO.1-blast shGAC

    Plasmid
    #110410
    Purpose
    shGAC (Target CCTCTGTTCTGTCAGAGTT), silence glutaminase isoform GAC, blasticidin selection.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 (RNA Pol III)
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    110410-plasmid-map-sequence-id-218291

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Showing: 2251 - 2300 of 7986 results