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20,205 results

Showing: 921 - 940 of 20205 results

  2. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    14 Articles
    61358-plasmid-map-sequence-id-99789

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  3. pCBC-DT1T2

    Plasmid
    #50590
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T1, U626t plus, U629p, T2
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    50590-plasmid-map-sequence-id-210724

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  5. pcDNA3.1(+)/Luc2=tdT

    Plasmid
    #32904
    Purpose
    Expresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.
    Depositor
    Christopher Contag
    Publication
    Patel et al Cancer Res. 2010 Dec 15;70(24):10141-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Firefly Luciferase
    tandem Tomato Red Fluorescent Protein
    Tags
    tdTomato red fluorescent protein
    Expression
    Mammalian
    Promoter
    CMV-IE and n/a
    Available since
    Feb. 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    26 Articles
    32904-plasmid-map-sequence-id-229770

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  15. pCMV14-3X-Flag-SARS-CoV-2 S

    Plasmid
    #145780
    Purpose
    Over expression of SARS-CoV-2 S (spike) in mammalian cell line
    Depositor
    Zhaohui Qian
    Publication
    Ou et al Nat Commun. 2020 Mar 27;11(1):1620. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S (S SARS CoV 2)
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Codon optimized and C-terminal 19 amino acids del…
    Promoter
    CMV
    Available since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    31 Articles
    145780-plasmid-map-sequence-id-354441

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  16. CROP-seq-opti

    Plasmid
    #106280
    Purpose
    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
    Depositor
    Jay Shendure
    Publication
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    27 Articles
    106280-plasmid-map-sequence-id-512176

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Showing: 921 - 940 of 20205 results