4,143 results

1. pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

   Plasmid

   #179105

   Purpose

   pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPRia-v2

   Plasmid

   #84832

   Purpose

   CRISPRi/a V2 library parental plasmid

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

   Inserts

   sgRNA
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmU6-gRNA

   Plasmid

   #53187

   Purpose

   Expresses the S. pyogenes sgRNA from the mouse U6 promoter

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-LC3

   Plasmid

   #11546

   Depositor

   Karla Kirkegaard

   Article

   Jackson et al PLoS Biol. 2005 May . 3(5):e156.

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #190034

   Purpose

   vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized hfCas13d
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A134V,A140V,A141V,A143V

   Promoter

   CBh, SV40, hU6

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lamp1-RFP

   Plasmid

   #1817

   Purpose

   Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.

   Depositor

   Walther Mothes

   Article

   Sherer et al Traffic 2003 Nov;4(11):785-801.

   Insert

   lysosome associated membrane protein 1 (Lamp1 Rat)
   

   Use

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   D50E (not important for function of plasmid)

   Promoter

   Available since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pR26 CAG/GFP Dest

   Plasmid

   #74281

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSF1389

   Plasmid

   #104962

   Purpose

   Bacterial expression plasmid of bdSENP1 protease

   Depositor

   Dirk Görlich

   Article

   Frey et al J Chromatogr A. 2014 Apr 11;1337:95-105.

   Insert

   SENP1
   

   Use

   Tags

   His14 tag and Tev protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Jan. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET302-6His-dCas9-Halo

   Plasmid

   #72269

   Purpose

   Encodes a Halo-labeled fusion of nuclease deficient Cas9

   Depositor

   Timothée Lionnet

   Article

   Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

   Insert

   His6-dCas9-Halo
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSIJ8

    Plasmid

    #68122

    Purpose

    Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

    Depositor

    Alex Nielsen

    Article

    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

    Inserts

    flp recombinase
    rhaS
    rhaR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhamnose

    Available since

    Dec. 8, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pJFRC7-20XUAS-IVS-mCD8::GFP

    Plasmid

    #26220

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    mCD8::GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. VCP(wt)-EGFP

    Plasmid

    #23971

    Depositor

    Nico Dantuma

    Article

    Tresse et al Autophagy. 2010 Feb 22. 6(2):.

    Insert

    Valosin containing protein (VCP Human)
    

    Use

    Tags

    6xHis, EGFP, and HA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMF230

    Plasmid

    #62546

    Purpose

    Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.

    Depositor

    Michael Franklin

    Article

    Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.

    Insert

    GFP mut2
    

    Use

    Broad host range plasmid

    Tags

    Expression

    Mutation

    Promoter

    trc

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-6xHis-SUMO-MarathonRT

    Plasmid

    #109029

    Purpose

    Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

    Depositor

    Anna Pyle

    Article

    Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

    Insert

    ERE_01490
    

    Use

    Tags

    6xHis-SUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACYC-GroEL/ES-TF

    Plasmid

    #83923

    Purpose

    Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Gro EL/ES
    Trigger Factor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-MmPeg10rc4

    Plasmid

    #174858

    Purpose

    Expresses recoded mouse Peg10 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    recoded Mus musculus PEG10 (Peg10 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. mTurquoise2-N1

    Plasmid

    #54843

    Purpose

    Localization: N1 Cloning Vector, Excitation: 434, Emission: 474

    Depositor

    Dorus Gadella, Michael Davidson

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pcDNA3.1(+)Flag-His-ATM wt

    Plasmid

    #31985

    Purpose

    Mammalian expression of human ATM with Flag and His tags

    Depositor

    Michael Kastan

    Article

    Canman et al Science. 1998 Sep 11;281(5383):1677-9.

    Insert

    ATM wild-type (ATM Human)
    

    Use

    Tags

    Flag and HIS

    Expression

    Mammalian

    Mutation

    3' UTR of 400 nucl.

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX304

    Plasmid

    #25890

    Purpose

    3rd generation lentiviral vector

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW57-CMV-ssRFP-GFP-KDEL

    Plasmid

    #128257

    Purpose

    Fluorescence reporter to monitor ER-phagy activity

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    ssRFPGFPKDEL
    

    Use

    Lentiviral

    Tags

    RFPGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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