We narrowed to 23,753 results for: Spr

1. pRS143

   Plasmid

   #122377

   Purpose

   CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC-U63-QtgRNA2.1-BR

   Plasmid

   #170513

   Purpose

   gRNA cloning vector containing a BFP Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6:3

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLH-sgRNA-Sirius-8XPP7

   Plasmid

   #121940

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   sgRNA-Sirius-8XPP7
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQCasTns(Ptr)-array 8

   Plasmid

   #175580

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

   Insert

   PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(8 CRISPR array)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NO

   Promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAFi-dHdrA

   Plasmid

   #184907

   Purpose

   carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

   Depositor

   Radhakrishnan Mahadevan

   Article

   Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

   Insert

   Cm-sgRNA-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AcrIIA4

   Plasmid

   #101043

   Purpose

   Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

   Depositor

   Zhiwei Huang

   Article

   Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

   Insert

   AcrIIA4
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSET-dCas9-actII-4-NT-S1

   Plasmid

   #110185

   Purpose

   Repression of gene expression in Streptomyces by CRISPRi

   Depositor

   Yinhua Lu

   Article

   Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

   Inserts

   dCas9
   sgRNA
   

   Use

   CRISPR; I

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   J23119 and ermE*p

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSBY1_FnCpf1cg

   Plasmid

   #104622

   Purpose

   CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1

   Depositor

   Sheng Yang

   Article

   Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

   Insert

   FnCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   codon-optimized C.glutamicum

   Promoter

   hsp60

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pxpr_502-wt-ltr

   Plasmid

   #182647

   Purpose

   for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF, contains intact LTR

   Depositor

   Jakob Reiser

   Article

   Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

   Insert

   None
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYZ301

    Plasmid

    #102690

    Purpose

    S. pombe gRNA entry vector with Cas9 - Sp lys9 marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRPaCas9

    Plasmid

    #111819

    Purpose

    Expresses Cas9 in T. brucei (2T1) cells

    Depositor

    David Horn

    Article

    Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYZ294

    Plasmid

    #102688

    Purpose

    S. pombe gRNA entry vector with Cas9 - KanMX marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYZ300

    Plasmid

    #102689

    Purpose

    S. pombe gRNA entry vector with Cas9 - Sp his3 marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLH-sgRNA1-boxB-MS2-PP7

    Plasmid

    #75397

    Purpose

    sgRNA1-boxB-MS2-PP7

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-boxB-MS2-PP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYZ293

    Plasmid

    #102687

    Purpose

    S. pombe gRNA entry vector with Cas9 - Sp leu1 marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pspCas9-3'UTR-ST2-com-vector

    Plasmid

    #136269

    Purpose

    Expresses SpCas9 and sgRNA Scaffold

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Insert

    SpCas9 and sgRNA scaffold
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SP6-sgRNA-scaffold

    Plasmid

    #47912

    Purpose

    Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection

    Depositor

    Paul Sternberg

    Article

    Chiu et al Genetics. 2013 Aug 26.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    sgRNA 3' end

    Expression

    Worm

    Mutation

    Promoter

    SP6

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. JME4390

    Plasmid

    #129656

    Purpose

    LEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSpCas9n(BB)-2A-puro-a5 guide B

    Plasmid

    #194011

    Purpose

    For CRISPR knockout of integrin alpha5

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (a5_guide B)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSpCas9n(BB)-2A-puro-aV guide A

    Plasmid

    #194008

    Purpose

    For CRISPR knockout of integrin alphaV

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (aV_guide A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSpCas9n(BB)-2A-puro-aV guide B

    Plasmid

    #194009

    Purpose

    For CRISPR knockout of integrin alphaV

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (aV_guide B)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSpCas9n(BB)-2A-puro-a5 guide A

    Plasmid

    #194010

    Purpose

    For CRISPR knockout of integrin alpha5

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (a5_guide A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEL081

    Plasmid

    #137890

    Purpose

    iSTU-CRISPR/Cas9 with OsCDPK2 intron, tRNA structure backbone

    Depositor

    Yong Zhang

    Article

    Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SpyCas9(WT)

    Plasmid

    #101044

    Purpose

    Express Streptococcus pyogenes Cas9

    Depositor

    Zhiwei Huang

    Article

    Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

    Insert

    SpyCas9
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLH-sgRNA1-PP7-boxB

    Plasmid

    #75393

    Purpose

    sgRNA1-PP7-boxB

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XPP7-boxB
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSDMA65

    Plasmid

    #128354

    Purpose

    Codon optimised Streptococcus pyogenes CAS9 ORF regulated by the Cryptococcus neoformans TEF1 promoter and terminator.

    Depositor

    James Fraser

    Article

    Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ACT1

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLH-sgRNA1-MS2-PP7

    Plasmid

    #75392

    Purpose

    sgRNA1-MS2-PP7

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XMS2-PP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLH-sgRNA1-boxB-MS2

    Plasmid

    #75396

    Purpose

    sgRNA1-boxB-MS2

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-boxB-MS2
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJYS2.1-crtYf

    Plasmid

    #89077

    Purpose

    Constitutive transcription of crRNA of crtYF. CRISPR of C. glutamicum.

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Insert

    crRNA of crtYF
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCS2-SauCas9

    Plasmid

    #174040

    Purpose

    In vitro transcription of SauCas9 mRNA

    Depositor

    James Gagnon

    Article

    Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

    Insert

    SauCas9
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge