We narrowed to 119,908 results for: CIL

1. pDest17_Gcn4 Aro2+

   Plasmid

   #231883

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 Aro2+

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

   Insert

   Gcn4 Aro2+
   

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   N116F, S117E, E119N, P129Q, T132Y

   Promoter

   T7

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDest17_Gcn4 WTSLFD

   Plasmid

   #231881

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 WTSLFD

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

   Insert

   Gcn4 WTSLFD
   

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   F108W, E109T, Y110S, E111L, N112F, L113D

   Promoter

   T7

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDest17_Gcn4 F108A+

   Plasmid

   #231875

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 F108A+

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

   Insert

   Gcn4 F108A+
   

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   F108A, T121L, A141P

   Promoter

   T7

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDest17_Gcn4 ILVtoED W

   Plasmid

   #231871

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED W

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

   Insert

   Gcn4 ILVtoED W
   

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   N126W, I128E, V130E, V135E, L137D, I142D

   Promoter

   T7

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDest17_Gcn4 ILVtoAS

   Plasmid

   #231859

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoAS

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

   Insert

   Gcn4 ILVtoAS
   

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   L123S, I128S, V130A, V135A, L137S, I142S

   Promoter

   T7

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDest17_Gcn4 ILVtoED

   Plasmid

   #231857

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

   Insert

   Gcn4 ILVtoED
   

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   L123D, I128E, V130E, V135E, L137D, I142D

   Promoter

   T7

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-hfYFP-TEV-Bcx

   Plasmid

   #191195

   Purpose

   Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   hfYFP & Bcx fusion
   

   Tags

   6xHis and Bacillus circulans xylanase

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_Myc-HalM-P2A-FLAG-YFast-TEV-His-HalB

   Plasmid

   #225064

   Purpose

   Expression of HalM-modified HalA2 in human cells

   Depositor

   Wilfred van der Donk

   Article

   Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

   Insert

   HalA2
   

   Tags

   Multiple tags due to polycistronic expression

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. BS-ZJLEU-PTEF1-GFP

   Plasmid

   #207018

   Purpose

   Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   GFP
   

   Expression

   Yeast

   Promoter

   pTEF1

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. PTPI1-Dsred-URA3

    Plasmid

    #207017

    Purpose

    Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    DsRed-Express 2
    

    Expression

    Yeast

    Promoter

    pTPI1

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. BS-TRP1-PGAL-GFP-Cps1

    Plasmid

    #207030

    Purpose

    Expression of GFP-Cps1 under GAL promoter.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Cps1
    

    Tags

    GFP

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. 3xMS2 TR knockin sgRNA 1

    Plasmid

    #207607

    Purpose

    sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    cgactcgcccggcagcgcac
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xMS2 TR knockin sgRNA 2

    Plasmid

    #207608

    Purpose

    sgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    acccccaaacctgactgact
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. TR knockout HRD

    Plasmid

    #207579

    Purpose

    Homologous recombination donor replacing TR with a puro cassette.

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    puro resistance cassette flanked by TR locus sequences excluding TR
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST14-Cys-SpyCatcher (Min)

    Plasmid

    #216713

    Purpose

    Expresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014

    Depositor

    Alex Dunn

    Article

    Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

    Insert

    Cys-SpyCatcher (Min)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See Li...Howarth, J. Mol. Biol., 2014

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PCUP1-4V5-Pho8-Met

    Plasmid

    #207029

    Purpose

    Expression of 4V5-Pho8 under CPU1 promoter.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Tags

    4xV5

    Expression

    Yeast

    Promoter

    pCUP1

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. BS-Trp-PCUP1-4myc-Pho8

    Plasmid

    #207028

    Purpose

    Expression of 4Myc-Pho8 under CPU1 promoter.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Tags

    4xMyc

    Expression

    Yeast

    Promoter

    pCUP1

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PCUP1-Vac8-GFP-Ura

    Plasmid

    #207027

    Purpose

    Expression of Vac8-GFP under CPU1 promoter.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Vac8
    

    Tags

    GFP

    Expression

    Yeast

    Promoter

    pCUP1

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PCUP1-GFP-Vma13-Ura

    Plasmid

    #207026

    Purpose

    Expression of GFP-Vma13 under CPU1 promoter.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Vma13 ORF
    

    Tags

    GFP

    Expression

    Yeast

    Promoter

    pCUP1

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. P1K-3HA-Atg8∆GR-MET15

    Plasmid

    #207033

    Purpose

    Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Tags

    3xHA

    Expression

    Yeast

    Mutation

    ∆GR

    Promoter

    pATG8

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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