We narrowed to 59,677 results for: plasmid
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Plasmid#30205PurposeLentiviral coexpression of Cre and puromycin from the human EF1a promoterDepositorInsertCre recombinase (cre Enterobacteria phage P1)
UseCre/Lox and LentiviralExpressionMammalianPromoterEF1alphaAvailable sinceApril 12, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC57-sgRNA expression vector
Plasmid#51132PurposeFor in vitro transcription of sgRNA from the T7 promoter.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterT7Available sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR
Plasmid#60226PurposeExpresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.DepositorInsertssgRNA
Renilla luciferase
Cre recombinase
UseAAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…TagsCre-HA and Rluc-P2AExpressionMammalianPromoterEFS and U6Available sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Puro.Cre empty vector
Plasmid#17408PurposeLentiviral expression of Cre from the mouse PGK promoterDepositorInsertCre
UseCre/Lox and LentiviralExpressionMammalianPromotermouse PGKAvailable sinceMarch 11, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-hSyn-Soma-jGCaMP8s
Plasmid#169256PurposeAAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8s.DepositorHas serviceAAV1, AAV9, and AAV RetrogradeInsertSoma-jGCaMP8s
UseAAVTags6xHis-tagExpressionMammalianAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRGEB32
Plasmid#63142PurposeExpress sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.DepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterRice snoRNA U3 promoter for gRNA/PTG expression a…Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FgH1tUTG
Plasmid#70183PurposeInducible expression of guide RNA with fluorescent GFP reporterDepositorInsertH1-Tet-sgrna cassette
UseCRISPR and LentiviralExpressionMammalianAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cbh_v5 AAV-ABE C-terminal
Plasmid#137178PurposeAAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE C-terminal; U6-protospacer
UseAAVPromoterCMVAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEcgRNA
Plasmid#166581PurposeCRISPR-Cas9-assisted genome editing in Escherichia coliDepositorInsertccdB
ExpressionBacterialAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiCRISPRv2 neo
Plasmid#98292PurposeVariant of lentiCRISPRv2 that confers G418 resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralPromoterEF-1aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-GfaABC1D-Cre-4x6T
Plasmid#196410PurposeAstrocytic expression of Cre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorHas serviceAAV5InsertCre
UseAAV; Astrocyte-selectiveExpressionMammalianPromoterCAG and GfaABC1DAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
paavCAG-pre-mGRASP-mCerulean
Plasmid#34910PurposepreDepositorHas serviceAAV5Insertpresynaptic mGRASP
UseAAVPromoterCAGAvailable sinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFR_CrPV_xb
Plasmid#11509DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFirefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
UseLuciferaseAvailable sinceMarch 15, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR5'_ubi
Plasmid#27320PurposeGateway entry vector containing 3.5 kb ubi promoter fragment, with L4 and R1 sequences for MultiSite Gateway cloningDepositorInsertzebrafish ubiquitin promoter (ubb Zebrafish)
UseMultisite gateway 5' entry vectorAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9(BB)-2A-Hygro
Plasmid#127763PurposeUsed for expression of sgRNA in mammalian cellsDepositorTypeEmpty backboneExpressionMammalianAvailable sinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL2-p21 promoter-Luc
Plasmid#33021DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertp21 promoter (CDKN1A Human)
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterp21Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pIS0
Plasmid#12178Purpose3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein productionDepositorTypeEmpty backboneUseLuciferaseExpressionMammalianAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEcCas
Plasmid#73227PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRNADepositorInsertsCas9
sgRNA
UseCRISPR; Red recombinaseExpressionBacterialPromoternative promoterAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-U6-sgRNA-hSyn-mCherry
Plasmid#87916PurposesgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)DepositorInsertmCherry
UseAAV and CRISPRExpressionMammalianPromoterhSynAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Ef1a-fDIO hChR2(H134R)-EYFP
Plasmid#55639PurposeFlp-Dependent ChR2-EYFPDepositorHas serviceAAV RetrogradeInserthChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMammalianPromoterEf1aAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by