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  4. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Article
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105829-plasmid-map-sequence-id-203267

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  5. pIRE4-Bpa

    Plasmid
    #155342
    Purpose
    Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.
    Depositor
    Irene Coin
    Article
    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15
    Insert
    humanized EBpaRS/BstYam
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK/U6
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155342-plasmid-map-sequence-id-310519

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  7. miReporter-PGK

    Plasmid
    #82477
    Purpose
    microRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.
    Depositor
    Pierre Neveu
    Article
    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi
    Insert
    H2B-mCherry and H2B-Citrine. HygroR
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82477-plasmid-map-sequence-id-163461

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  43. pIRESpuro2 AXL

    Plasmid
    #65627
    Purpose
    Expresses AXL on cistronic transcript with puro resistance in mammalian cells
    Depositor
    Aaron Meyer
    Article
    Meyer et al Cell Syst. 2015 Jul 29;1(1):25-36.
    Insert
    Tyrosine-protein kinase receptor UFO (AXL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Spurious PDPRPHR on C terminus
    Promoter
    CMV
    Available since
    June 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    65627-plasmid-map-sequence-id-115157

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Showing: 201 - 250 of 18426 results