We narrowed to 2,200 results for: dcas9

1. pdCas9-Tet1-CD

   Plasmid

   #83340

   Purpose

   To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

   Depositor

   Ronggui Hu

   Article

   Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

   Insert

   dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   U6, CBH

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. dCas9-CBP FL

   Plasmid

   #179550

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpdCas9 VPR

   Plasmid

   #188509

   Purpose

   Expresses FLAG tagged SpdCas9 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pdCas9- AsiA_m2.1

   Plasmid

   #158065

   Purpose

   Expressing evolved CasTA of dCas9 fused AsiA variant 2.1

   Depositor

   Harris Wang

   Article

   Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

   Insert

   dCas9-AsiA_m2.1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetO

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGH125_dCas9-Blast

   Plasmid

   #85417

   Purpose

   lentiviral expression vector for dCas9 with Blasticidin selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   dCas9 and Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330A_dCas9-1x4

   Plasmid

   #63598

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZ8-T_dCas9

   Plasmid

   #74062

   Purpose

   pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   dcas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ptac

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ezh2[FL]-dCas9

   Plasmid

   #100086

   Purpose

   Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   14056 (Ezh2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-Ezh2[FL]-dCas9-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCas9-humanized

   Plasmid

   #44246

   Purpose

   Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dead Cas9 with 3X NLS
   

   Use

   CRISPR and Retroviral; Vector

   Tags

   3x NLS

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   MSCV LTR promoter

   Available Since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. LLP791_PB_dCas9-SSSavi_BirA

    Plasmid

    #211786

    Purpose

    PiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    dCas9, Spy, Snoop, aGCN4, AviTags
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    dCas9 D10A and H840A

    Promoter

    pCAGG abd pEF1a

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpdCas9 KRAB

    Plasmid

    #188499

    Purpose

    Expresses FLAG tagged SpdCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/H840A

    Promoter

    EF1a

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. dCas9-GCN5 FL

    Plasmid

    #179552

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 (KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdCas9-omega

    Plasmid

    #114632

    Purpose

    Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

    Depositor

    Anushree Chatterjee

    Article

    Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

    Insert

    dCas9-omega
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet promoter

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO5d.SFFV.dCas9-KRAB.P2A.BSD

    Plasmid

    #90332

    Purpose

    CRISPR interference

    Depositor

    Dirk Heckl

    Article

    Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

    Inserts

    dCas9
    na
    SFFV
    P2A-BSD
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330A_dCas9-1x7

    Plasmid

    #63601

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. M3-M14-dCas9

    Plasmid

    #134779

    Purpose

    Expression of fusion protein M3-M14-dCas9 in Mammalian cells

    Depositor

    Shu-Bing Qian

    Article

    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

    Insert

    METTL3/METTL14 methyltransferase domain and inactive dCas9 (METTL3, METTL14 S. pyogenes, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBbdCas9S_Psyn-sgRNA500

    Plasmid

    #149640

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pScI_dCas9-CDA-UL

    Plasmid

    #108551

    Purpose

    Bacterial Target-AID UGI-LVA vector

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Insert

    dCas9-PmCDA1-UGI-LVA
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    lambda OR

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXPR_051_dCas9-KRAB-HA

    Plasmid

    #228936

    Purpose

    All-in-one dCas9-KRAB-MeCP2 plasmid for cloning of custom sgRNA

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQdCas9-sgempty

    Plasmid

    #159523

    Purpose

    Expression of dCas9 driven by lux cassette from Vibrio fischeri

    Depositor

    Radhakrishnan Mahadevan

    Article

    Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021

    Insert

    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    quorum sensing promoter

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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