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Showing: 2501 - 2550 of 9178 results

  2. p007ampGFP

    Plasmid
    #58535
    Purpose
    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    Green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T5 between two lac operators
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58535-plasmid-map-sequence-id-90574

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  3. pNO183

    Plasmid
    #101068
    Purpose
    ppsR2-bphP1 bicistronic operon plasmid
    Depositor
    Jeffrey Tabor
    Article
    Ong et al ACS Synth Biol. 2017 Nov 1. doi: 10.1021
    Inserts
    ppsR2 (ppsR2 Rhodopseudomonas palustris CGA009)
    bphP1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    insert guanine at position 430 to fix frameshift …
    Promoter
    Available since
    Nov. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    101068-plasmid-map-sequence-id-194033

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  6. pKB222

    Plasmid
    #170012
    Purpose
    Encodes LacI-controlled expression of surface-displayed LL-37 and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    LL-37
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    March 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    170012-plasmid-map-sequence-id-335945

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  10. pE_Cre_lambdaCos

    Plasmid
    #173785
    Purpose
    The pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.
    Depositor
    Keith Shearwin
    Article
    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102
    Insert
    Cre
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mutation
    S102Y and S279Y- please see depositor comments
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    173785-plasmid-map-sequence-id-342369

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  11. pMARO

    Plasmid
    #178086
    Purpose
    Derivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.
    Depositor
    Sören Abel
    Article
    Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10
    Insert
    TcR (tetA/tetR)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Tetracycline resistance cassette is flanked by Ec…
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    178086-plasmid-map-sequence-id-349998

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  12. pKB230

    Plasmid
    #170017
    Purpose
    Encodes LacI-controlled expression of surface-displayed protegrin-1 and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    Protegrin-1
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    170017-plasmid-map-sequence-id-339960

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  13. pKB226

    Plasmid
    #170016
    Purpose
    Encodes LacI-controlled expression of surface-displayed defensin HNP-1 and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    Defensin HNP-1
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    170016-plasmid-map-sequence-id-335960

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  14. pAC574

    Plasmid
    #127975
    Purpose
    AMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    hygB (hygromycin resistance marker)
    Use
    Fungal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans trpC promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    127975-plasmid-map-sequence-id-254311

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  18. COVID-SARS2 NSP16/10

    Plasmid
    #159615
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10
    Depositor
    Opher Gileadi
    Inserts
    Covid-SARS2 Nsp16
    Covid-SARS2 Nsp10
    Use
    Tags
    His6-Zb-TEV and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159615-plasmid-map-sequence-id-311066

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  19. p006kanGFP

    Plasmid
    #58534
    Purpose
    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    Green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T5 between two lac operators
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58534-plasmid-map-sequence-id-90573

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  28. pZH516

    Plasmid
    #102669
    Purpose
    Constitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102669-plasmid-map-sequence-id-199392

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  33. pZH514

    Plasmid
    #102667
    Purpose
    Constitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    102667-plasmid-map-sequence-id-199386

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  38. pST50Trc1-HISNDHFR

    Plasmid
    #63940
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis; TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63940-plasmid-map-sequence-id-177346

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  41. pECT3

    Plasmid
    #75450
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75450-plasmid-map-sequence-id-146478

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  42. pZH520

    Plasmid
    #102673
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102673-plasmid-map-sequence-id-199588

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  43. pZH521

    Plasmid
    #102674
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102674-plasmid-map-sequence-id-199585

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  45. pZH517

    Plasmid
    #102670
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102670-plasmid-map-sequence-id-199582

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  48. pKB225

    Plasmid
    #170015
    Purpose
    Encodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    Peptide display protein with no C-terminal peptide
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Nov. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    170015-plasmid-map-sequence-id-335287

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Showing: 2501 - 2550 of 9178 results