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HA-MAP1LC3B

Plasmid

#137757

Purpose

Expression of LC3B

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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AiP15043 - pAAV-AiE1419m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN5043)

Plasmid

#224189

Purpose

AiE1419m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+/Pthlh+ neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCR1316

Plasmid

#111126

Purpose

Expresses FANCA.

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCA (FANCA Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_BFP

Plasmid

#25891

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

BFP

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pATZ-Myc

Plasmid

#177718

Purpose

ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant), myc tagged

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Use

Tags

Myc

Expression

Bacterial and Mammalian

Mutation

E342K (ATZ mutant)

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCWori cytochrome P450-BM3h 9D7

Plasmid

#61308

Purpose

Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

Depositor

Alan Jasanoff

Article

Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

Insert

Cytochrome P450-BM3 heme domain 9D7

Use

Tags

6xHis

Expression

Bacterial

Mutation

T268A, I263A, T438V, A328G

Promoter

Tac

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pDZ Flag USP28

Plasmid

#15665

Depositor

Martin Eilers

Article

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

USP28 (USP28 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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FAS_pLX307

Plasmid

#98334

Purpose

Lentiviral expression of FAS

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

FAS (FAS Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19/Mito-mChe

Plasmid

#183167

Purpose

Construct for transient expression of mitochondrion-targeted mCherry in plants.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

Mito-mCherry

Use

Tags

OsCOX11, Oryza sativa cytochrome c oxidase assemb…

Expression

Plant

Mutation

Promoter

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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mT-Sapphire-N1

Plasmid

#54569

Purpose

Localization: N1 Cloning Vector, Excitation: 399, Emission: 511

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al Nat Methods. 2008 May . 5(5):401-3.

Type

Empty backbone

Use

Tags

mT

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pRS410

Plasmid

#11258

Depositor

Fred Cross

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 24, 2006

Availability

Academic Institutions and Nonprofits only

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pTALYM3B15

Plasmid

#47878

Purpose

TALE-mClover against mouse major satellite sequence

Depositor

Maria-Elena Torres-Padilla

Article

Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

Insert

TALE-mClover

Use

TALEN

Tags

mClover

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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LcV2-Hygro

Plasmid

#91977

Purpose

LentiCRISPR v2 with Hygro cloned in place of Puro

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_BE4max

Plasmid

#112093

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

BE4max

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-miRFP704nano

Plasmid

#206190

Purpose

Near-infrared fluorescent protein miRFP704nano in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Protein Sci. 2023 Jun 22:e4709. doi: 10.

Insert

miRFP704nano

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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phU6-gRNA

Plasmid

#53188

Purpose

Expresses the S. pyogenes sgRNA from the human U6 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pET22b-CPDSalI

Plasmid

#38251

Depositor

Aimee Shen, Matthew Bogyo

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Use

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-DYRK1A

Plasmid

#101770

Purpose

Mammalian expression of DYRK1A fusion protein

Depositor

Michael Huen

Article

Li et al Nucleic Acids Res. 2024 Jul 5:gkae574. d

Insert

DYRK1A (DYRK1A Human)

Use

Tags

S peptide-flag-streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

siRNA-resistant allele

Promoter

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-CFP II (pMIC II)

Plasmid

#52109

Purpose

a bicistronic IRES-CFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Type

Empty backbone

Use

Retroviral

Tags

IRES-ECFP

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-EGxxFP-Cetn1

Plasmid

#50717

Purpose

Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Article

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Insert

Cetn1 (Cetn1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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