164,577 results

1. pRG646_lexO-HO_LexA-TF_LEU2MX

   Plasmid

   #154815

   Purpose

   Inducible expression of HO endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   HO endonuclease (HO Budding Yeast)
   LexA-ER-B112
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. mScarletI_MapTauC

   Plasmid

   #112964

   Purpose

   In vivo visualization of microtubules (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   MapTauC
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe RFP1-KRT5 hygro

   Plasmid

   #58493

   Purpose

   mammalian expression of mRFP1-tagged human KRT5

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   KRT5 (KRT5 Human)
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pEGFP-MYO7A-TAIL

   Plasmid

   #89585

   Purpose

   Expression plasmid for a positive control bait protein in NanoSPD 2.0 assays.

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYO7A (Myo7a Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncation encoding amino acids 870-2166 of MYO7A…

   Promoter

   CMV

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX302 GDF11-V5 puro

   Plasmid

   #83097

   Purpose

   Lentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   GDF11 (GDF11 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

6. pESC-MET

   Plasmid

   #222482

   Purpose

   Co-expression of two genes in yeast with FLAG and MYC tags and MET15 selection

   Depositor

   Tetsuo Matsuzaki, Kiyofumi Yamada

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   FLAG and Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pESC-LYS

   Plasmid

   #222483

   Purpose

   Co-expression of two genes in yeast with FLAG and MYC tags and LYS2 selection

   Depositor

   Tetsuo Matsuzaki, Kiyofumi Yamada

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   FLAG and Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. BFP-Rab5CA (Q79L)

   Plasmid

   #205705

   Purpose

   Mammalian expression of BFP-tagged mutant Rab5CA

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   RAB5A (RAB5A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q79L

   Promoter

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2193

   Plasmid

   #141964

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TFAM (TFAM Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-MYRIP

    Plasmid

    #89586

    Purpose

    Expression plasmid for a positive control prey protein in NanoSPD 2.0 assays.

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYRIP (Myrip Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAd/CMV/DAX1-IRES-nEGFP

    Plasmid

    #29752

    Depositor

    Edward McCabe

    Insert

    dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (NR0B1 Human)
    

    Use

    Tags

    EGFP, IRES, and Nuclear localization sequence

    Expression

    Mammalian

    Mutation

    No mutations; Reporter is nuclear localized EGFP;…

    Promoter

    Available Since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1_gRNA

    Plasmid

    #196139

    Purpose

    gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    AAVS1 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-N-Puro-mStayGold-ACTB

    Plasmid

    #227320

    Purpose

    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-MYO10-HMM-Nanotrap

    Plasmid

    #87255

    Purpose

    Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO10 (Myo10 Mouse)
    

    Use

    Tags

    GFP-nanotrap

    Expression

    Mammalian

    Mutation

    Myo10 is truncated to contain aa.1-941

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFlag-CMV2-Brd4 del ET

    Plasmid

    #21938

    Depositor

    Eric Verdin

    Article

    Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa 1-1362 only, ET domain deleted

    Promoter

    Available Since

    Sept. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pLV CMV FST-FLAG

    Plasmid

    #175167

    Purpose

    Lentiviral expression of human FST-FLAG

    Depositor

    Larry Gerace

    Insert

    FST (FST Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-HsBIN1_tv8

    Plasmid

    #176012

    Purpose

    Mammalian expression of Human EGFP-Bin1 (transcriptional variant 8, muscle specific). Parton lab clone HBH

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAB167

    Plasmid

    #101672

    Purpose

    Opto-T7RNAP*(563), equal expr.

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    Opto-T7RNAP*(563), equal expr.
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223_FYN_WT

    Plasmid

    #82211

    Purpose

    Gateway Donor vector containing FYN , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    FYN (FYN Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS40P

    Plasmid

    #35481

    Depositor

    Steven Haase

    Article

    Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

    Type

    Empty backbone

    Use

    Yeast integrating vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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