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Plasmid#179693PurposeMutagenesis plasmid with constitutive bacterial expression of DddI immunity proteinDepositorInsert[SD8] gIII
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
P223
Plasmid#139720PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, CPHDepositorInsertpMOD_A'_pLHB1B1:NnCPH:tSlH4
UseLuciferaseTagsExpressionPlantMutationPromoterAtLHB1B1Available sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBT100.110
Plasmid#122602Purposeclone CDA1 phage by SapI Golden GateDepositorInsertCDA1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
FR_luc
Plasmid#31098DepositorInsertfirefly luciferase
UseFlp/frtTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
EZ-L767
Plasmid#131773PurposePADH1_sFUS_Fusion Red_PixD_TACT1DepositorInsertPADH1_sFUS_Fusion Red_PixD_TACT1
UseIntegration into δ-sitesTagsExpressionYeastMutationPromoterPADH1Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT362
Plasmid#127844Purposeclone BE4max-NG CBEs by SapI Golden GateDepositorInsertBE4max-NG without deaminase
UseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pdx-PH2/pGL3
Plasmid#12163DepositorInsertPdx1 promoter PH2 domain
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJune 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-STV1-iGFP
Plasmid#177111PurposeExpresses Stv1-iGFP in yeast cellsDepositorInsertSTV1 (STV1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDC
Plasmid#166941PurposeDual control based multi-level gene expression controller (MLC)DepositorInsertCircuit expressing GFP- Dual Control
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF-01
Plasmid#166931PurposeGFP coding region part (F)DepositorInsertGreen Fluorescent Protein - Block F- Flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-CaURA3
Plasmid#162461PurposeDNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaURA3DepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-CaHIS1
Plasmid#162462PurposeDNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-Ca HIS1DepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shMTH1-1
Plasmid#21297DepositorInsertshMTH1-1 (NUDT1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCfB2192
Plasmid#67539PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSec7-Halox2
Plasmid#115425PurposeExpress Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertSec7-Halox2
UseTagsHaloTagExpressionYeastMutationPromoterEndogenousAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4I
Plasmid#104504PurposeUsed to evaluate the expression output of Saccharomyces kudriavzevii GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSkGAL1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4Q
Plasmid#104512PurposeUsed to evaluate the expression output of Saccharomyces kudriavzevii GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSkGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4L
Plasmid#104507PurposeUsed to evaluate the expression output of Saccharomyces mikatae GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSmGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT100.136c
Plasmid#122603Purposeclone FERNY phage by SapI Golden GateDepositorInsertFERNY
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-vps10-104
Plasmid#115426PurposeGenerate the vps10-104 allele via pop-in pop-out mutagenesisDepositorInsertvps10-104
UseTagsExpressionYeastMutationdomain 1 deletionPromoterEndogenousAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJEC578
Plasmid#174366PurposedCas9 for modular CRISPRa. SYNZIP-dCas9. Expresses dCas9 with a SYNZIP18 domain fused to the N terminus.DepositorInsertdCas9 fused to SYNZIP18
UseTagsExpressionBacterialMutationPromoterJ23150Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB2195
Plasmid#67548PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4H
Plasmid#104503PurposeUsed to evaluate the expression output of Saccharomyces arboricola GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSaGAL1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4J
Plasmid#104505PurposeUsed to evaluate the expression output of Saccharomyces mikatae GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSmGAL1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4N
Plasmid#104509PurposeUsed to evaluate the expression output of Saccharomyces bayanus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSbGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4R
Plasmid#104513PurposeUsed to evaluate the expression output of Saccharomyces uvarum GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSuGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB2194
Plasmid#67545PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae X-4 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBM13h-sd4U
Plasmid#179690PurposeBacterial expression of base editorDepositorInsert[sd4U] UGI-TALE3-DddA-G1397-N wildtype
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB2513
Plasmid#67543PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shMTH1-2
Plasmid#21298DepositorInsertshMTH1-2 (NUDT1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtinGW
Plasmid#133377PurposePiggybac vector for constitutive expressionDepositorTypeEmpty backboneUseTagsFlagExpressionMammalianMutationPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo
Plasmid#115421PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFREE2-eSpCas9-sgRNA
Plasmid#179580PurposeExpresses eSpCas9 and gRNA that targets ColE1 plasmids for curingDepositorInserteSpCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
EZ-L498
Plasmid#131771PurposePPGK1_FUSN_Citrine _PixE_TCYC1DepositorInsertPPGK1_FUSN_Citrine _PixE_TCYC1
UseIntegration into his3 locusTagsExpressionYeastMutationPromoterPGKAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCO489
Plasmid#139732PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, LuzDepositorInsertpMOD_D_AtuMas:NnLuz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromoterAtuMASAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB2400
Plasmid#67552PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked dsdA marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0058
Plasmid#117539PurposeLevel1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamianaDepositorInsertsgRNA (SaCas9)
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
PABE-2
Plasmid#115623PurposeTargeted A to G in wheat plants or monocotyledons protoplastsDepositorInsertwtTadA-TadA7.10-nCas9-NLS
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3
Plasmid#112658PurposeExpression S.cerevisiaeDepositorInsertsfGFP-Glo3
UseTagssfGFPExpressionYeastMutationPromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB2198
Plasmid#67554PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2228
Plasmid#67540PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0007
Plasmid#117534PurposeLevel0 Golden Gate part: CDS, LbCas12aDepositorInsertLbCas12a (with stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDS66
Plasmid#48364Purpose3XHA-intergenic region of TUB-HYG (modified from PMID 16269191)DepositorInsert3XHA-intergenic region of TUB-HYG (modified from PMID 16269191)
UseCre/Lox; Knockout in t. bruceiTagsExpressionMutationPromoterAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDS67
Plasmid#48365Purpose3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)DepositorInsert3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)
UseCre/Lox; Knockout in t. bruceiTagsExpressionMutationPromoterAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSec7-iGFPx6
Plasmid#115424PurposeExpress Sec7-iGFPx6 at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertSec7-iGFPx6
UseTagsiGFPExpressionYeastMutationPromoterEndogenousAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMV-δ1L9'S-nAchR
Plasmid#20687DepositorInsertnicotinic acetylcholine receptors
UseTagsAMV and pA 50ExpressionBacterialMutationLeucine at 9' position in M2 region changed …PromoterAvailable sinceApril 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRL1058
Plasmid#70686PurposeTn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.DepositorInsertTransposon Tn5-1058
UseTagsExpressionMutationPromoterAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBT195
Plasmid#127843Purposeclone BE4max CBEs by SapI Golden GateDepositorInsertBE4max without deaminase
UseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
PABE-3
Plasmid#115624PurposeTargeted A to G in wheat plants or monocotyledons protoplastsDepositorInsertwtTadA-TadA7.10-NLS-nCas9-NLS
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
PABE-6
Plasmid#115627PurposeTargeted A to G in wheat plants or monocotyledons protoplastsDepositorInsertwtTadA-TadA7.10-nCas9-2*NLS
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only