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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 9,223 results for: Coli

Showing: 1681 - 1710 of 9223 results

  4. Plasmid EUPp_09

    Plasmid
    #157745
    Purpose
    PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)
    Depositor
    Kang Zhou
    Article
    Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(
    Inserts
    acetaldehyde dehydrogenase
    alcohol dehydrogenase (ADH2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    June 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    157745-plasmid-map-sequence-id-334102

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  6. pVDL9.3

    Plasmid
    #168299
    Purpose
    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA
    Depositor
    Víctor de Lorenzo
    Article
    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do
    Insert
    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    LacI-Plac
    Available since
    June 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    168299-plasmid-map-sequence-id-335286

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  7. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

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  10. pPM1003

    Plasmid
    #167550
    Purpose
    Encodes genes that confer expression of Vibrio cholerae O antigens of the Ogawa serotype in E. coli K-12
    Depositor
    Renato Morona
    Article
    Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:
    Insert
    Vibrio cholerae O antigens genes of the Ogawa serotype
    Use
    Cosmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    167550-plasmid-map-sequence-id-476338

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  14. pFDH

    Plasmid
    #131706
    Purpose
    Express constitutively an NAD-dependent formate dehydrogenase from Pseudomonas sp. 101 in E. coli
    Depositor
    Ron Milo
    Article
    Gleizer et al Cell. 2019 Nov 27;179(6):1255-1263.e12.
    Insert
    formate dehydrogenase
    Use
    Tags
    his-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    131706-plasmid-map-sequence-id-258662

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  15. pJBEI-6411

    Plasmid
    #47050
    Purpose
    BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli
    Depositor
    Taek Soon Lee
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Insert
    ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
    Use
    Synthetic Biology; Bglbrick
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pbad
    Available since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    47050-plasmid-map-sequence-id-67072

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  16. pTrc-trGPPS(CO)-LS

    Plasmid
    #50603
    Purpose
    pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase
    Depositor
    Jay Keasling
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Inserts
    Limonene Synthase
    ApR
    LacIq
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    truncated codon optimized geranylpyrophosphate sy…
    Promoter
    Trc
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    50603-plasmid-map-sequence-id-77114

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  19. pET28a-His-MinD_MinE

    Plasmid
    #133621
    Purpose
    Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.
    Depositor
    Petra Schwille
    Article
    Loose et al Science. 2008 May 9;320(5877):789-92. do
    Insert
    His-MinD_MinE (minD, minE E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133621-plasmid-map-sequence-id-271463

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  20. pPM1001

    Plasmid
    #167548
    Purpose
    Encodes genes that confer expression of Vibrio cholerae O antigens of the Inaba serotype in E. coli K-12
    Depositor
    Renato Morona
    Article
    Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:
    Insert
    Vibrio cholerae O antigens genes of the Inaba serotype
    Use
    Cosmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    167548-plasmid-map-sequence-id-333912

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  21. pCRISPReporter

    Plasmid
    #65007
    Purpose
    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65007-plasmid-map-sequence-id-109898

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  22. pCDF-NtXNtS1R1AtR2NtB2

    Plasmid
    #160881
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    rbcS-S1, rbcX, raf1, raf2, bsd2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-S1: T7, chaperones: T7
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160881-plasmid-map-sequence-id-318841

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  25. pCDF-NtXR1AtR2B2NtS1

    Plasmid
    #160879
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    rbcS-S1, rbcX, raf1, raf2, bsd2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-S1: pBAD, chaperones: T7
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160879-plasmid-map-sequence-id-318813

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  26. pCDF-NtXNtS1R1AtR2B2

    Plasmid
    #160880
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana rubisco chaperone proteins raf2, and bsd2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    rbcS-S1, rbcX, raf1, raf2, bsd2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-S1: T7, chaperones: T7
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160880-plasmid-map-sequence-id-318839

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  28. pCDF-NtXNtTcR1AtR2NtB2

    Plasmid
    #160889
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-Tc), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    PT7-Nt-rbcS-Tc
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-Tc: T7, chaperones: T12
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160889-plasmid-map-sequence-id-318862

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  29. pTE1059

    Plasmid
    #186628
    Purpose
    Expression of S. coelicolor butyrolactone biosynthesis genes in E. coli. Heterologous production of SCBs.
    Depositor
    Eriko Takano
    Article
    Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10
    Inserts
    scbA
    scbB
    scbC
    Use
    Synthetic Biology
    Tags
    6xHis and 6xHis-thrombin
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic promoter B10, Synthetic promoter B19, a…
    Available since
    Sept. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    186628-plasmid-map-sequence-id-371218

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  30. pCDF-NtXNtT2R1AtR2NtB2

    Plasmid
    #160886
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-T2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    PT7-Nt-rbcS-T2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-T2: T7, chaperones: T9
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160886-plasmid-map-sequence-id-318849

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Showing: 1681 - 1710 of 9223 results