We narrowed to 18,831 results for: Tec

1. pColE1_sgRNA_pos8_AmpR

   Plasmid

   #119151

   Purpose

   Encodes sgRNA targeting position 8 in pColE1_70a_deGFP_KanR

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 8 in pColE1_70a_deGFP_KanR
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. U6-petRNA

   Plasmid

   #181802

   Purpose

   petRNA cloning plasmid

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

   Insert

   petRNA-Kan
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEL852

   Plasmid

   #214252

   Purpose

   Expression TadCBEd_V106W in rice cells

   Depositor

   Yong Zhang

   Article

   Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCM4.4

   Plasmid

   #217648

   Purpose

   an ermE*p-cas9-driven expression, as an alternative plasmid

   Depositor

   Huimin Zhao

   Article

   Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

   Insert

   Codon Optimized SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p105_gRNA_Sa_CD40

   Plasmid

   #213781

   Purpose

   Expresses CRISPRa gRNA for CD40 promoter and mScarlet. gRNA driven by mU6.

   Depositor

   Howard Chang

   Article

   Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   Sa gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mU6

   Available Since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMJH65

   Plasmid

   #67273

   Purpose

   Conjugally transferable red recombinogenic plasmid

   Depositor

   Mark Liles

   Article

   Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

   Insert

   tetA
   

   Use

   Red recombinase, temperature sensitive

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetA promoter of plasmid pACYC184 origin

   Available Since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cas9m4

   Plasmid

   #47316

   Purpose

   Cas9 D10A+D839A+H840A+N863A

   Depositor

   George Church

   Article

   Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

   Insert

   Cas9m4
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBBR1k-J23101-GFPuv

   Plasmid

   #106388

   Purpose

   pBBR1k-GFPuv with Anderson promoter J23101 expressing lacI

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available Since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPR::rpsL

   Plasmid

   #44505

   Purpose

   A crRNA expression plasmid specific to the rpsL allele.

   Depositor

   Luciano Marraffini

   Article

   Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   CRISPR::rpsL (rpsL )
   

   Use

   CRISPR; E.coli

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. CJ8421_04975 Rptr2 recording plasmid

    Plasmid

    #194211

    Purpose

    CJ8421_04975 Rptr2 targeted DNA sequence

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    CJ8421_04975 Rptr2 DNA target
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEL851

    Plasmid

    #214251

    Purpose

    Expression TadCBEd in rice cells

    Depositor

    Yong Zhang

    Article

    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEL855

    Plasmid

    #214255

    Purpose

    Expression Td-CBEmax in rice cells

    Depositor

    Yong Zhang

    Article

    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. DL146_plenti_TRE_GFP-iPAK4

    Plasmid

    #187446

    Purpose

    Tet-On inducible expression of eGFP-iPAK4 in a lentiviral backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    INKA-box-Pak4
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTS1106_Tier3(Lenti)

    Plasmid

    #169661

    Purpose

    Tier-3 vector for stable integration of up to three cassettes via lentiviral transduction (PCMV-5'LTR-Psi-gag-env-A1::A2::A3-WPRE-3'LTR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL193

    Plasmid

    #180471

    Purpose

    For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

    Depositor

    Peggy Lemaux

    Article

    Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Cas9

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 6his-WT gusA-pET28a+

    Plasmid

    #61156

    Purpose

    N -terminal His tagged wild-type GUS expression vector

    Depositor

    Ichiro Matsumura, Andrew Ellington

    Article

    Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

    Insert

    gusA
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE-EFS-PCP-3XNeon-nls

    Plasmid

    #75386

    Purpose

    PCP-3XNeon-nls

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    PCP
    

    Use

    Lentiviral

    Tags

    3XmNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLH-sgRNA1-2XboxB

    Plasmid

    #75391

    Purpose

    sgRNA1-2XboxB

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XboxB
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GGE114

    Plasmid

    #120731

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Destination vector for the insertion of the assembly. It already has some of the parts needed for transformation in Y. lipolytica

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Type

    Empty backbone

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-FLAG-Luc-N

    Plasmid

    #58790

    Purpose

    Express LucN alone in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    firefly Luciferase 1-398
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T576C,T591C to destroy MfeI and EcoRI sites

    Promoter

    CMV

    Available Since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJBL3752

    Plasmid

    #128809

    Purpose

    Fluoride riboswitch-regulated sfGFP

    Depositor

    Julius Lucks

    Article

    Thavarajah et al ACS Synth Biol. 2019 Dec 12. doi: 10.102

    Insert

    Fluoride riboswitch-regulated sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PiggyBac-G13-sensor

    Plasmid

    #137809

    Purpose

    A FRET sensor for G13 activation, encoded on a single plasmid

    Depositor

    Dorus Gadella

    Article

    Bordes et al Methods Mol Biol. 2021;2268:159-178. doi

    Insert

    Galpha13 sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBBR2k-GFPuv

    Plasmid

    #106383

    Purpose

    Anhydrotetracycline-inducible tet promoter expressing gfpuv on BBR1 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tet

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a-NLS-M-MLV-RT

    Plasmid

    #181804

    Purpose

    pET28a-NLS-M-MLV RT

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

    Insert

    NLS-M-MLV-RT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSBbi-BN

    Plasmid

    #60518

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKD182

    Plasmid

    #90952

    Purpose

    Expresses ThsS under the Ptac promoter

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Insert

    ThsS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEL853

    Plasmid

    #214253

    Purpose

    Expression TadDE in rice cells

    Depositor

    Yong Zhang

    Article

    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. DL145_plenti_TRE_iPAK4

    Plasmid

    #187445

    Purpose

    Tet-On inducible expression of iPAK4 in a lentiviral backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    INKA-box-Pak4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLZ12Km2-P23R:TA:fflucRT

    Plasmid

    #88901

    Purpose

    Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

    Depositor

    Thomas Proft

    Article

    Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

    Insert

    toxin-antitoxin + Red-shifted firefly luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMAL-p4X

    Plasmid

    #75289

    Purpose

    Expresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

    Depositor

    Paul Riggs

    Article

    Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

    Type

    Empty backbone

    Use

    Tags

    maltose-binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    July 19, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pLZ12Km2-P23R:TA:ffluc

    Plasmid

    #88900

    Purpose

    Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

    Depositor

    Thomas Proft

    Article

    Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

    Insert

    toxin-antitoxin + firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLJM1-zeo-UL32

    Plasmid

    #162664

    Purpose

    Lentiviral vector for stable expression of UL32 in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    UL32
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pB1H1

    Plasmid

    #12611

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Insert

    Dorsal RHR in pB1H1 (dl Fly)
    

    Use

    Tags

    flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. EasyFusion mAID-mCherry with Neo

    Plasmid

    #113831

    Purpose

    EasyFusion vector for targeting endogeneous genes with mAID-mCherry

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    mAID-mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. UbC-TRE-mSOD2-tTS-IRES-EGFP

    Plasmid

    #12390

    Depositor

    Zuoshang Xu

    Article

    Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

    Insert

    mSOD2 shRNA (Sod2 Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCfB2223

    Plasmid

    #67544

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBad33_ISAs26_reRNA

    Plasmid

    #205326

    Purpose

    Bacterial expression of ISAs26 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISAs26 TnpB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET-gSTEP1

    Plasmid

    #159376

    Purpose

    Expresses gSTEP1 in E. coli

    Depositor

    Roberto Chica

    Article

    Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

    Insert

    gSTEP1
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. EMM67

    Plasmid

    #49043

    Purpose

    Backbone contains SHD/G 0.5 Domain TALE N-terminal TALE C-terminus, LSD1: Isoform 2

    Depositor

    Eric Mendenhall, Bradley Bernstein

    Article

    Mendenhall et al Nat Biotechnol. 2013 Sep 8. doi: 10.1038

    Type

    Empty backbone

    Use

    Tale lsd1 expression vector

    Tags

    LSD1

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 alpha

    Available Since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PlayBack-U6-Xba1

    Plasmid

    #203300

    Purpose

    For expression of sgRNAs in a PlayBack Vector with XbaI as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge