We narrowed to 4,845 results for: phy

1. mEos2-Fascin-C-10

   Plasmid

   #57376

   Purpose

   Localization: Actin Bundling Protein, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   Fascin (FSCN1 Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pEPSA5Cas9syn

   Plasmid

   #206965

   Purpose

   S. aureus genome editing vector removed these two motifs AGGNNNNNGAT and CCAYNNNNNNTGT

   Depositor

   Daijie Chen

   Article

   Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLv-CMV-MyoD

   Plasmid

   #26808

   Depositor

   Jeffrey Chamberlain

   Article

   Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

   Insert

   MyoD (Myod1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGUS (GB0208)

   Plasmid

   #68203

   Purpose

   Provides the CDS of the β-glucuronidase gene as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   GUS coding region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. VinculinTS-A50I/I997A

   Plasmid

   #111829

   Purpose

   Vinculin tension sensor with point mutations affecting ability to bind talin and actin

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (I997A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed vinculin alanine 50 to isoleucine and iso…

   Promoter

   CMV

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET30b-5M SrtA with TEV site

   Plasmid

   #86962

   Purpose

   Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

   Depositor

   Ron Bose

   Article

   Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

   Insert

   S.aureus SrtA 5M
   

   Use

   Tags

   6x Histidine tag and TEV site

   Expression

   Bacterial

   Mutation

   P94R, D160N, D165A, K190E, K196T

   Promoter

   T7

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-tagRFP-NLS-HA-Bxb1

   Plasmid

   #65625

   Purpose

   Vector for cotranslational expression of tagRFP-Bxb1

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Bxb1 integrase
   

   Use

   Tags

   HA, SV40 NLS, and tagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-smURFP-T2A-mCherry

   Plasmid

   #80347

   Purpose

   Lentiviral transfer vector under the CMV promoter that expresses smURFP and mCherry.

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   smURFP
   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRU206

   Plasmid

   #167693

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   PcUBQ4-2::asCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRU322

    Plasmid

    #167683

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::asCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRSET FLII12Pglu-700uDelta6

    Plasmid

    #13568

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    FLIPglu F16A Y12S-ECFP-D13R, 6aa linker
    

    Use

    Tags

    Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in mglB

    Promoter

    Available since

    Feb. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CROP_C9_Puro_DNMT3B

    Plasmid

    #183284

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets DNMT3B gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    DNMT3B
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRRL-VinculinTS-A50I

    Plasmid

    #111831

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind talin in lentiviral expression vector

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (A50I) (VCL Chicken)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    changed vinculin alanine 50 to isoleucine

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-HA-ERK2 D319N

    Plasmid

    #8979

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D319N

    Promoter

    Available since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pSW457 - tCLV3

    Plasmid

    #115980

    Purpose

    entry vector for GreenGate cloning method, terminator

    Depositor

    Jan Lohmann

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    tCLV3
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSW455 - pCLV3

    Plasmid

    #115979

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Jan Lohmann

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pCLV3
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVL26 - pSCR

    Plasmid

    #116021

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pSCR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    T171A by SDM to abolish Eco31I site

    Promoter

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3-deltaBamHI-human MIZ1

    Plasmid

    #74167

    Purpose

    Mammalian expression of human MIZ1

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    MIZ1 (ZBTB17 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pSW614 - pREV

    Plasmid

    #115989

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pREV
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR-PpU6P-sgRNA-L5L2

    Plasmid

    #113738

    Purpose

    Gateway entry vector containing attL5 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    P. patens U6 promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSW618 - pAHP6

    Plasmid

    #115991

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pAHP6
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSW615 - pATHB-8

    Plasmid

    #115990

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pATHB-8
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    A1287T to abolish Eco31I site

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pVL15 - tSCR

    Plasmid

    #116030

    Purpose

    entry vector for GreenGate cloning method, terminator

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    tSCR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CROP_C9_Puro_DNMT3A

    Plasmid

    #183283

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets DNMT3A gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    DNMT3A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pENTR-PpU6P-sgRNA-R4R3

    Plasmid

    #113739

    Purpose

    Gateway entry vector containing attR4 and attR3 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    P. patens U6 promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENTR-PpU6P-sgRNA-L5L4

    Plasmid

    #113741

    Purpose

    Gateway entry vector containing attL5 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    P. patens U6 promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pENTR-PpU6P-sgRNA-L1R5

    Plasmid

    #113737

    Purpose

    Gateway entry vector containing attL1 and attR5 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    P. patens U6 promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CROP_C9_Puro_PARP1

    Plasmid

    #183312

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets PARP1 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    PARP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Dendra2-LaminB1-10

    Plasmid

    #57728

    Purpose

    Localization: Nuclear Envelope, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

30. BbZIP-cALFA

    Plasmid

    #160498

    Purpose

    For the protein expression of BbZIP with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    BbZIP
    

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. MAC_C_INSR

    Plasmid

    #187772

    Purpose

    MAC-tagged gene expression of human INSR

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    INSR (INSR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. SLF060: Lenti-CaMKIIa-jRGECO1a

    Plasmid

    #112282

    Purpose

    For lentiviral or direct expression of cytosolic jRGECO1a in excitatory neurons

    Depositor

    Adam Cohen

    Article

    Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

    Insert

    jRGECO1a
    

    Use

    Lentiviral

    Tags

    NES-6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIa

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pQCXIP-HA.Tetherin

    Plasmid

    #41069

    Depositor

    Paul Spearman

    Article

    Hammonds et al PLoS Pathog. 2010 Feb 5;6(2):e1000749.

    Insert

    Tetherin (BST2 Human)
    

    Use

    Retroviral

    Tags

    HA and IRES Puro

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/MSV

    Available since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pG3H-U6SC

    Plasmid

    #134755

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    gRNA-U6, zCas9-35SC

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GLUT9-eGFP/pcDNA-DEST47

    Plasmid

    #18730

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    GLUT9-eGFP/pcDNA-DEST47 (SLC2A9 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted TAA

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

36. MAC_C_EPHB4

    Plasmid

    #187768

    Purpose

    MAC-tagged gene expression of human EPHB4

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    EPHB4 (EPHB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pPAiRFP2-N1

    Plasmid

    #44273

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Nat Commun. 2013 Jul 10;4:2153. doi: 10.

    Insert

    PAiRFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K69R/R83K/G120D/A123T/M163L/Q168E/R220P/S243N/V24…

    Promoter

    CMV

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. YTIP-GFP

    Plasmid

    #42238

    Depositor

    William Pu

    Article

    von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

    Insert

    YTIP-GFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pENTR-PpU6P-sgRNA-L1L2

    Plasmid

    #113735

    Purpose

    Gateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    P. patens U6 promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pARR5::LUC

    Plasmid

    #141170

    Purpose

    Luminescent reporter for cytokinin signaling

    Depositor

    Vardis Ntoukakis, Patrick Schäfer

    Article

    Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

    Insert

    AtARR5 promoter
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtARR5, AT3G48100

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge