171,035 results
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Plasmid#222632PurposeRho inactivation using rapamycinDepositorInsertmCherry-FKBP-GAP(ARHGAP29) (ARHGAP29 Human)
TagsmCherry-FKBPExpressionMammalianPromoterCAGAvailable SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-T4gp43(WT)
Plasmid#228203PurposeExpression in BL21(DE3) of WT T4 DNA polymeraseDepositorInsert43
TagsSce VMA intein-chitin binding domainExpressionBacterialPromoterT7Available SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spCas9 ABE-Umax-ex2-rest1
Plasmid#222143PurposeAdenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9
UseCRISPR; Zebrafish expressionTagsBipartite NLSPromoterT3Available SinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PFKL
Plasmid#23729DepositorInsertPFKL (PFKL Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)
Plasmid#71485PurposeBase plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon armsDepositorTypeEmpty backbonePromoterU6Available SinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mEGFP-VPS4A
Plasmid#116924Purposeexpresses mEGFP-VPS4A in mammalian cellsDepositorInsertVPS4A (VPS4A Human)
UseRetroviralTagsmEGFPExpressionMammalianMutationsilent mutation in position 4059 C to TPromoterCMVAvailable SinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hSOX2
Plasmid#17218PurposeRetroviral expression of human SOX2DepositorAvailable SinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Cx43-7
Plasmid#55023PurposeLocalization: Gap Junctions, Excitation: 587, Emission: 610DepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
EE-mut SV40 luc
Plasmid#8460DepositorInserthuman cyclin E promoter fragment, mutant (CCNE1 Human)
UseLuciferaseExpressionMammalianMutationE2F sites I, II, and III mutated.Available SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
PGK-AID-NTERM-GFP
Plasmid#185773PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, AIDMutationnonePromoterPGKAvailable SinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BirA*-dCas9-eGFP
Plasmid#84779PurposeExpresses BirA*-dCas9-eGFP in mammalian cells.DepositorInsertBirA*-dCas9-eGFP
UseCRISPRTagscMycExpressionBacterial and MammalianPromoterCAGAvailable SinceJuly 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD40-His/V5-c-MycT58A
Plasmid#45598DepositorAvailable SinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSupAR-MbPylRS(DiZPK)
Plasmid#91705Purposeexpress Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interestDepositorInsertspylS
pylT
ExpressionBacterialMutationL274A, C313SAvailable SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag TSC2 wt
Plasmid#12132DepositorAvailable SinceJuly 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR
Plasmid#60231PurposeExpresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.DepositorInsertssgRNA
Cre recombinase
EGFP
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HA-P2A and EGFP-KASHExpressionMammalianPromoterU6 and hSynAvailable SinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAB_PalkB_sfgfp_KanR
Plasmid#166503PurposeExpression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialPromoterPalkBAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET151-D-TOPO-PARP14-SΔK
Plasmid#216658PurposeExpresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.DepositorInsertPARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Synthetic, Human)
Tags6xHis-V5-TEVExpressionBacterialMutationDeletion starting from Q1390 to D1448 and substit…PromoterT7Available SinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Daxx/pEGFP
Plasmid#119021PurposeExpress GFP-Daxx in mamalian cellsDepositorAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCBP1_Full-length_pGEX
Plasmid#135108PurposeRecombinant expression of PCBP1 with dual-affinity tagDepositorAvailable SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p7SL-neo-TET
Plasmid#51285Purposeexpresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassetteDepositorInsert7SL RNA (RN7SL2 Human)
TagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianPromoter7SL upstream pollII enhancerAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only