Addgene

New search

Keep filters

4,045 results

Showing: 1771 - 1800 of 4045 results

Results per page:

Sort results by:

1036 pcDNA3 Myr HA Akt1

Plasmid

#9008

Purpose

Mammalian expression of human Akt1 with myr, HA tags

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Use

Tags

HA and myr

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3 NSE promoter

Plasmid

#11606

Depositor

Rosalyn Adam

Article

Kim et al Endocrinology. 2004 Feb . 145(2):613-9.

Insert

NSE promoter (ENO2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-ADAM17-HA

Plasmid

#65105

Purpose

Expression of HA tagged ADAM17 (TACE) in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

ADAM17 (ADAM17 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL-TRBO-G

Plasmid

#80083

Purpose

An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence and expresses GFP.

Depositor

John Lindbo

Article

Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

Insert

GFP

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pC035 - dLwCas13a-msfGFP

Plasmid

#91925

Purpose

Expresses catalytically inactive LwCas13a for mammalian RNA targeting

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

dLwCas13a

Use

CRISPR

Tags

NLS-3xHA and NLS-msfGFP

Expression

Mammalian

Mutation

R488A and R1060A

Promoter

EF-1a

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-AAV-syn-jGCaMP7f-WPRE

Plasmid

#104488

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9

Insert

jGCaMP7f

Use

AAV

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
eSpCas9(1.1)_No_FLAG_AAVS1_T2

Plasmid

#79888

Purpose

Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR

Tags

Untagged eSpCas9(1.1)

Expression

Mammalian

Mutation

Promoter

CBh

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL-MS2-6X

Plasmid

#27118

Depositor

Robert Singer

Article

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

MS2 binding sites (6)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2/HB

Plasmid

#26557

Depositor

Perry Hackett

Insert

pT2/HB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV/SB10

Plasmid

#24551

Depositor

Perry Hackett

Article

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

Sleeping Beauty

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP VAMP2

Plasmid

#42308

Depositor

Thierry Galli

Article

Martinez-Arca et al Proc Natl Acad Sci U S A. 2003 Jul 22;10

Insert

VAMP 2

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-bleo IGF-1R

Plasmid

#11212

Depositor

Ronald Kahn

Article

Entingh-Pearsall et al J Biol Chem. 2004 Sep 3. 279(36):38016-2

Insert

IGF-1R (IGF1R Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriEx-mCherry-LOV2

Plasmid

#81041

Purpose

Mammalian or insect expression for wt LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV, p10

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-dCas9-VP64

Plasmid

#47107

Purpose

Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Insert

dCas9-VP64

Use

CRISPR

Tags

Flag, HA, SV40 NLS, and VP64

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive)

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
DsRed-rab11 DN

Plasmid

#12680

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Use

Tags

DsRed and HA

Expression

Mammalian

Mutation

changed serine 25 to asparagine

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCXLE-hMLN

Plasmid

#27079

Purpose

Non-integrating (episomal) expression of human C-MYC, LIN28 and NANOG

Depositor

Shinya Yamanaka

Article

Okita et al Nat Methods. 2011 May;8(5):409-12

Insert

C-MYC, LIN28, NANOG (MYC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Myc-ASC

Plasmid

#73952

Purpose

Express c-Myc-tagged human ASC in mammalian cells

Depositor

Christian Stehlik

Article

Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

Insert

PYD and CARD domain containing, PYCARD (PYCARD Human)

Use

Tags

c-myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS578_DD-dSpyCas9-mCherry-APEX2

Plasmid

#108570

Purpose

Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Insert

dSpyCas9

Use

CRISPR and Lentiviral

Tags

APEX2, Destabilization domains, Flag, and mCherry…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-puro-ARID1A

Plasmid

#39478

Purpose

Tetracycline-inducible lentiviral expression of human ARID1A; 3rd generation lentiviral vector

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

ARID1A (ARID1A Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiPGK Puro DEST p38KTRClover

Plasmid

#59152

Purpose

Lentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVS1-P-CAG-GFP

Plasmid

#80491

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

CAG-GFP

Use

Donor vector (human)

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPomyces-2

Plasmid

#61737

Purpose

Streptomyces expression of codon-optimized Cas9 and custom gRNA

Depositor

Huimin Zhao

Article

Cobb et al ACS Synth Biol. 2014 Dec 8.

Inserts

sSpCas9
gRNA cassette

Use

CRISPR

Tags

Expression

Bacterial

Mutation

BbsI-flanked lacZ cassette inserted in place of s…

Promoter

gapdhp(EL) and rpsL(XC)-BbsI

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
MAVS-WT

Plasmid

#52135

Purpose

Retroviral vector for expression of human MAVS in mammalian cells

Depositor

Jonathan Kagan

Article

Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20

Insert

MAVS (MAVS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-Ubiquitin-K29R

Plasmid

#17602

Purpose

Mammalian expression of HA tagged ubiquitin K29R

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K29R; V5 deleted

Promoter

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_TRE3G-LSD1-dCas9-P2A-mCherry

Plasmid

#138462

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal LSD1-dCas9 fusion protein and mCherry

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

LSD1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-puro-GFP-progerin

Plasmid

#17663

Depositor

Tom Misteli

Article

Scaffidi et al Nat Cell Biol. 2008 Mar 2. ():.

Insert

GFP-D50 lamin A (LMNA Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Delta 50

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pE-FLP

Plasmid

#45978

Depositor

Keith Shearwin, Drew Endy

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Insert

flp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

flp driven by the constitutive promoter pE from p…

Promoter

pE (from phage P2)

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pmEGFP_a_tubulin_C1

Plasmid

#21039

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Use

Tags

pmEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-HA-USP8

Plasmid

#22608

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP8 (USP8 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pDY0663 ACTB atgRNA with v2 scaffold

Plasmid

#179108

Purpose

ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge