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Showing: 2361 - 2400 of 5867 results

  4. pYZ714

    Plasmid
    #132955
    Purpose
    crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombe
    Depositor
    Jef Boeke
    Article
    Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578
    Insert
    dFnCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS and nucleoplasmin NLS
    Expression
    Mutation
    Promoter
    adh1
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    132955-plasmid-map-sequence-id-307974

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  6. pMB66

    Plasmid
    #47946
    Purpose
    Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter
    Depositor
    Mike Boxem
    Article
    Waaijers et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP
    Expression
    Worm
    Mutation
    Promoter
    hsp-16.48
    Available since
    Dec. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    47946-plasmid-map-sequence-id-68806

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  9. pMB67

    Plasmid
    #47947
    Purpose
    Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter
    Depositor
    Mike Boxem
    Article
    Waaijers et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and egl-13 NLS
    Expression
    Worm
    Mutation
    Promoter
    hsp-16.48
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    47947-plasmid-map-sequence-id-68808

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  19. pCHW00015

    Plasmid
    #222446
    Purpose
    Level 0, C terminal tag module (TTCG-GCTT) containig VP64-NLS.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Insert
    VP64-NLS
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222446-plasmid-map-sequence-id-454075

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  20. pCHW00014

    Plasmid
    #222445
    Purpose
    Level 0, C terminal tag module (TTCG-GCTT) containing VP16-NLS.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Insert
    VP16-NLS
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    Available since
    Nov. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    222445-plasmid-map-sequence-id-453909

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  21. Efg1-ΔDBD-Q443P

    Plasmid
    #216401
    Purpose
    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutation
    Depositor
    Nicolas Fawzi
    Article
    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0
    Insert
    Efg1-ΔDBD-Q443P
    Use
    Tags
    6xHis-tags
    Expression
    Bacterial
    Mutation
    changed Glutamine 443 to Proline, and deleted ami…
    Promoter
    Available since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    216401-plasmid-map-sequence-id-430102

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  22. pAAV/CMV-SCLM

    Plasmid
    #216758
    Purpose
    AAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleon
    Depositor
    George Augustine
    Insert
    SuperClomeleon followed by WPRE and human growth hormone polyA terminator
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    CMV (cytomegalovirus)
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216758-plasmid-map-sequence-id-429489

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  23. pYPQ300

    Plasmid
    #205414
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Ev1Cas12a without promoter
    Depositor
    Yiping Qi
    Article
    Li et al Front Genome Ed. 2023 Oct 12;5:1251903.
    Insert
    Ev1Cas12a
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS (N terminal on insert) NLS (C terminal on ins…
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    205414-plasmid-map-sequence-id-414101

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  35. pJH127

    Plasmid
    #162483
    Purpose
    SEC plasmid containing LG1 homology arms and myo-2p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.
    Depositor
    Bob Goldstein
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Use
    CRISPR
    Tags
    myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    162483-plasmid-map-sequence-id-320838

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  36. pJH130

    Plasmid
    #162484
    Purpose
    SEC plasmid containing LG1 homology arms and myo-3p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.
    Depositor
    Bob Goldstein
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    myo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Use
    CRISPR
    Tags
    myo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    162484-plasmid-map-sequence-id-320834

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  38. pJH126

    Plasmid
    #162482
    Purpose
    SEC plasmid containing LG1 homology arms and vha-8p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.
    Depositor
    Bob Goldstein
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Use
    CRISPR
    Tags
    vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    162482-plasmid-map-sequence-id-320829

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  40. enOgeuIscB with ωRNA-v13

    Plasmid
    #220940
    Purpose
    CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v13 scaffold with BsmBI for guide cloing
    Depositor
    Weixin Tang
    Article
    Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/
    Inserts
    CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
    OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220940-plasmid-map-sequence-id-443125

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Showing: 2361 - 2400 of 5867 results