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Addgene

We narrowed to 6,962 results for: tet on

Showing: 2951 - 3000 of 6962 results
  1. MLM3733

    Plasmid
    #49964
    Purpose
    Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Insert
    3x Flag Tet1CD HB-6 (HBB Human)
    Use
    TALEN
    Tags
    3x FLAG
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
  2. JA1261

    Plasmid
    #49963
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Insert
    Tet1CD HB-6 (HBB Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
  3. JA1290

    Plasmid
    #49968
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Insert
    Tet1CD HB-9 (HBB Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
  4. JA1252

    Plasmid
    #49956
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Insert
    Tet1CD HB-3 (HBB Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
  5. JA1280

    Plasmid
    #49969
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Insert
    Tet1CD HB-10 (HBB Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
  6. JA1272

    Plasmid
    #49967
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Insert
    Tet1CD HB-8 (HBB Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
  7. JA1266

    Plasmid
    #49966
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Insert
    Tet1CD HB-7 (HBB Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
  8. JA1229

    Plasmid
    #49955
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Insert
    Tet1CD HB-2 (HBB Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
  9. JA1217

    Plasmid
    #49954
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Insert
    Tet1CD HB-1 (HBB Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
  10. pAJE3-E7

    Plasmid
    #214359
    Purpose
    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins
    Depositor
    Inserts
    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    AmpR and lpp
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. JA412

    Plasmid
    #49935
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site
    Depositor
    Insert
    Tet1CD KL-1 (KLF4 Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
  12. SL357

    Plasmid
    #49936
    Purpose
    Expresses a TALE-TET1FL (full length TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site
    Depositor
    Insert
    Tet1FL KL-1 (KLF4 Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
  13. JA431

    Plasmid
    #49937
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site
    Depositor
    Insert
    Tet1CD KL-2 (KLF4 Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
  14. pTACT13

    Plasmid
    #24784
    Depositor
    Insert
    PAg85a-TetR (tetR Bacterial)
    Use
    Mycobacterial shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2010
    Availability
    Academic Institutions and Nonprofits only
  15. pEG302-SunTagng-22aa

    Plasmid
    #115345
    Purpose
    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome
    Depositor
    Insert
    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  16. p2B-56

    Plasmid
    #177925
    Purpose
    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
    Depositor
    Insert
    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    V9I; F67S; G72P, F86Y; R171K
    Promoter
    MYO2
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  17. p2B-55

    Plasmid
    #177924
    Purpose
    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    Depositor
    Insert
    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    V9I; F67S; G72P, F86Y; R171K
    Promoter
    TDH3
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  18. pcDNA3.1 VDAC1-TC

    Plasmid
    #211733
    Purpose
    Expresses tetracysteine tagged VDAC1 in mammalian cells. TC-tag is attached to the C-terminus of VDAC1.
    Depositor
    Insert
    Voltage-dependent anion channel 1 (VDAC1 )
    Use
    Tags
    Tetracysteine
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
  19. pMJH65

    Plasmid
    #67273
    Purpose
    Conjugally transferable red recombinogenic plasmid
    Depositor
    Insert
    tetA
    Use
    Red recombinase, temperature sensitive
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tetA promoter of plasmid pACYC184 origin
    Available since
    Sept. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
  20. pES133

    Plasmid
    #127552
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13
    Insert
    F5-flanked tetracycline resistance cassette
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Multiple SNPs in tetA (originally from pBR322) to…
    Promoter
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
  21. pCL27-decoder

    Plasmid
    #159449
    Purpose
    Insert: Pfus1tet-citrine-tCyc1. The fluorescent protein Citrine is inducible by alpha-factor and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.
    Depositor
    Insert
    Pfus1tet-citrine-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Pfus1tet
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  22. pCL28-decoder

    Plasmid
    #159450
    Purpose
    Insert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.
    Depositor
    Insert
    PlexAtet-LexA-ER-B112-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PlexAtet
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  23. pET-Sfp R4-4 mutant

    Plasmid
    #173491
    Purpose
    Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.
    Depositor
    Insert
    Sfp-R44
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  24. pS5

    Plasmid
    #231614
    Purpose
    L-arabinose (Ara)- and anhydrotetracycline (aTc)-inducible inverter circuit reported by mKate and/or sfGFP fluorescence signals
    Insert
    Pbad-TetR-sfGFP-Ptet-AraC-mKate2
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pbad/Ptet
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
  25. pS4

    Plasmid
    #231613
    Purpose
    L-arabinose (Ara)- and anhydrotetracycline (aTc)-inducible inverter circuit reported by mKate and/or sfGFP fluorescence signals
    Insert
    Ptet-AraC-mKate2-Pbad-TetR-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pbad/Ptet
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
  26. pCL38-decoder

    Plasmid
    #159452
    Purpose
    Insert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2
    Depositor
    Insert
    Pfus1tet-LexA-ER-B112-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Pfus1tet
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  27. pCL12-decoder

    Plasmid
    #159447
    Purpose
    Insert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.
    Depositor
    Insert
    PlexAtet-Citrine-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PlexAtet
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  28. pCMT-flp

    Plasmid
    #67274
    Purpose
    Conjugally transferable Flp recombinase plasmid for unmarked mutagenesis
    Depositor
    Insert
    tetA, flp, repA101, oriR101
    Use
    Flp recombinase plasmid, temperature sensitive
    Tags
    His-SUMO (flp)
    Expression
    Mutation
    Promoter
    tetA promoter of plasmid pACYC184 origin
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
  29. pTS2163

    Plasmid
    #169632
    Purpose
    Tier-3 vector for CRISPR-mediated stable integration into the AAVS1 locus of the tetON-system (OTetO7-PCMVmin-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::A3-pA)
    Depositor
    Insert
    OTetO7-PCMVmin-SEAP-iRFP670::PmPGK1-rtTA-pA::A3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO7-PhCMVmin / PPGK
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 2951 - 3000 of 6962 results