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We narrowed to 14,607 results for: pET

Showing: 3001 - 3050 of 14607 results

  1. pET28-Nsp14-TEV-6His

    Plasmid
    #154952
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Nsp14-TEV-6His (ORF1ab SARS-CoV-2)
    Use
    Tags
    TEV-6His
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154952-plasmid-map-sequence-id-307820

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  2. pET23b-Casp2-Delta Card

    Plasmid
    #11810
    Depositor
    Guy Salvesen
    Insert
    Caspase-2 Delta Card (CASP2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Delta Card
    Promoter
    Available since
    May 12, 2006
    Availability
    Academic Institutions and Nonprofits only

  6. pET-29b(+)_NSP4ss_SerM_NucA-myc

    Plasmid
    #218191
    Purpose
    Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)
    Depositor
    Florence Pojer
    Insert
    nucA
    Use
    Tags
    NSP4 signal secretion peptide and myc
    Expression
    Bacterial
    Mutation
    codon optimized ecoli residues - 22-266
    Promoter
    T7
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218191-plasmid-map-sequence-id-433865

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  9. pET151-D-TOPO-PARP14-SΔK

    Plasmid
    #216658
    Purpose
    Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.
    Depositor
    Herwig Schüler
    Article
    Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.
    Insert
    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Synthetic, Human)
    Use
    Tags
    6xHis-V5-TEV
    Expression
    Bacterial
    Mutation
    Deletion starting from Q1390 to D1448 and substit…
    Promoter
    T7
    Available since
    Aug. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    216658-plasmid-map-sequence-id-429340

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  10. pET28a_DmKHC[1-421]-1xGFP-6xHis

    Plasmid
    #196972
    Purpose
    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag
    Depositor
    Lukas Kapitein
    Article
    Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Use
    Tags
    6xHis and GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196972-plasmid-map-sequence-id-388918

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  15. pET-alpha-synuclein-BiFC

    Plasmid
    #87855
    Purpose
    pETDUET-1 based vector for BiMolecular Fluorescence Complementation based assays of alpha-synuclein oligomerisation
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187
    Inserts
    alpha-synuclein-VC155
    alpha-synuclein-VN154
    Use
    Tags
    C-terminal fragment of mVenus BiFC protein and N-…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87855-plasmid-map-sequence-id-175017

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  18. pET28a_DmKHC[1-421]-SNAP-6xHis

    Plasmid
    #196975
    Purpose
    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag
    Depositor
    Lukas Kapitein
    Article
    Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Use
    Tags
    6xHis and SNAP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196975-plasmid-map-sequence-id-388920

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  34. pET28a-TGFα-GSY-SPM

    Plasmid
    #163615
    Purpose
    Expresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.
    Depositor
    Mark Howarth
    Article
    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:
    Insert
    TGFa-GSY-SPM (TGFA Synthetic, Human)
    Use
    Tags
    C-tag and His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    163615-plasmid-map-sequence-id-323634

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  37. pET28-6His-SUMO-Nsp14

    Plasmid
    #154956
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
    Use
    Tags
    6His-Thrombin-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154956-plasmid-map-sequence-id-307845

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  42. pcDNA3-AMPKsub-YPet-NES

    Plasmid
    #84635
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub-YPet-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    84635-plasmid-map-sequence-id-176207

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  49. pET30a-His6-ABP-LOXCATmut

    Plasmid
    #139972
    Purpose
    A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)
    Depositor
    Vamsi Mootha
    Article
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutation in H265A and R268A in LOX (used original…
    Promoter
    T7
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    139972-plasmid-map-sequence-id-311162

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Showing: 3001 - 3050 of 14607 results