12,913 results

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R26-H2B-mCherry HR donor vector

Plasmid

#137928

Purpose

R0SA26 knock-in vector with homologous recombination-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-H2B-mCherry-bpA with homology arms

Use

Mouse Targeting

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT-DEST EVC2-Flag

Plasmid

#41005

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
PB-TAC-OK+9MS-Cas9

Plasmid

#120354

Purpose

piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

Depositor

Knut Woltjen

Article

Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

Insert

OK+9MS cassette

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Mutation

Klf4 [1-483]

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-EGFP-ELYS-polyA

Plasmid

#59746

Purpose

Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

EGFP-ELYS

Tags

EGFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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Syndapin2-pmCherryC1

Plasmid

#27681

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Syndapin 2

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
pMX-mTAF4b

Plasmid

#44345

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF4b (Taf4b Mouse)

Use

Retroviral

Tags

flag and SBP

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF13B

Plasmid

#134626

Purpose

Mammalian expression of mouse KIF13B 442-1843

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF13B (Kif13b Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT

Promoter

Chicken Beta actin

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-MCS-shCHD5 #1

Plasmid

#68876

Purpose

CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5

Use

AAV and RNAi

Expression

Mammalian

Promoter

H1

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pLV V5-lamin B2

Plasmid

#175109

Purpose

Lentiviral expression of V5-tagged mouse Lmnb2

Depositor

Larry Gerace

Insert

Lmnb2 (Lmnb2 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe(puro) mouse Egr2

Plasmid

#108097

Purpose

Retroviral vector for expression of mouse Egr2

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr2 (Egr2 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Bet1

Plasmid

#120243

Purpose

Expresses V5-tagged Bet1 in lentiviral vector

Depositor

Larry Gerace

Insert

Bet1 (Bet1 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-HA tagged-Mouse Mdm2 wt-IRES-GFP

Plasmid

#180491

Purpose

Role of Mdm2 splicing variants in tumorigenesis

Depositor

Martine Roussel

Article

Dang et al Cancer Res. 2002 Feb 15;62(4):1222-30.

Insert

mouse Mdm2 full length (wt)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAd PdxI-I-nGFP

Plasmid

#19411

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

Insert

pancreatic and duodenal homeobox 1 (Pdx1 Mouse)

Use

Adenoviral; Entry vector

Tags

GFP and IRES

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
pPyCAG-Nanog-IP

Plasmid

#13838

Depositor

Shinya Yamanaka

Article

Mitsui et al Cell. 2003 May 30. 113(5):631-42.

Insert

Nanog (Nanog Mouse)

Expression

Mammalian

Mutation

None

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pHIV-Wnt1HA

Plasmid

#18983

Depositor

Bryan Welm, Zena Werb

Article

Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

Insert

wingless-related MMTV integration site 1 (Wnt1 Mouse)

Use

Lentiviral

Tags

HA-Tag

Expression

Mammalian

Available Since

Sept. 24, 2008

Availability

Academic Institutions and Nonprofits only
pAAV.TBG.PI.FLPo.rBG

Plasmid

#236288

Purpose

AAV vector expressing FLPo from TBG promoter

Depositor

Melina Fan, Addgene Research Program

Has Service

AAV8

Insert

FLPo

Use

AAV

Promoter

TBG

Available Since

May 20, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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LMP AMPK alpha1 hp1

Plasmid

#89489

Purpose

shRNA against AMPK alpha1 in mouse/human to knock down protein expression

Depositor

Russell Jones

Article

Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

Insert

hairpin against mouse/human AMPKalpha1 (PRKAA1, Prkaa1 Mouse, Human)

Use

Mouse Targeting and Retroviral

Expression

Mammalian

Promoter

U6 (The GFP-Puro is from a PGK promoter; the shRN…

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only
mSIRT3-L

Plasmid

#33309

Depositor

Michael Sack

Article

Bao et al J Cell Biochem. 2010 May;110(1):238-47.

Insert

Sirt3 (Sirt3 Mouse)

Tags

3XFlag

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-MSulf-1

Plasmid

#13007

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

mouse sulfatase 1 (Sulf1 Mouse)

Expression

Mammalian

Available Since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only
pScalps_puro_mIL-2Rα

Plasmid

#59917

Purpose

Expresses mouse interleukin-2 receptor alpha chain

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

interleukin 2 receptor alpha (Il2ra Mouse)

Use

Lentiviral

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
p-EGFP-C1-Flag-mFmr1(D)

Plasmid

#87914

Purpose

Expresses mouse FMRP-EGFP fusion with phosphorylation mimic S499D.

Depositor

Stephanie Ceman

Article

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Insert

Fmr1 (Fmr1 Mouse)

Tags

Flag and eGFP

Expression

Mammalian

Mutation

S499D

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mFzd2

Plasmid

#42254

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 2 (Fzd2 Mouse)

Expression

Mammalian

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-SO

Plasmid

#20329

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-F2A-Oct4

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pCalpha EV (PKA catalytic subunit Calpha)

Plasmid

#15310

Depositor

Stanley McKnight

Article

Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7

Insert

PRKACA (Prkaca Mouse)

Expression

Mammalian

Available Since

July 6, 2007

Availability

Academic Institutions and Nonprofits only
Anti-Pan-PTPR [N165/43R]

Plasmid

#188219

Purpose

Mammalian Expression Plasmid of anti-Pan-PTPR. Derived from hybridoma N165/43

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Pan-PTPR recombinant mouse monoclonal antibody (PTPRF Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CRY2olig-mCh-CLC

Plasmid

#60035

Purpose

Expresses a fusion of CRY2olig (PHR, E490G) with mCherry and mouse clathrin light chain

Depositor

Chandra Tucker

Article

Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

Insert

CRY2-mCh-Clathrin Light Chain (CRY2 Mustard Weed, Mouse)

Expression

Mammalian

Mutation

M354I, E490G CRY2, A9D*

Promoter

CMV

Available Since

Oct. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Stat3

Plasmid

#139805

Purpose

doxycycline-inducible expression of mouse Stat3 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Stat3 (Stat3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 KSR2

Plasmid

#25968

Depositor

Rob Lewis

Article

Costanzo-Garvey et al Cell Metab. 2009 Nov . 10(5):366-78.

Insert

Kinase suppressor of Ras 2 (Ksr2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pLenti-TRE-PtenWT-Blast

Plasmid

#135674

Purpose

Dox-inducible expression (TRE promoter) of wildtype Pten cDNA, Blasticidin selection

Depositor

Florian Karreth

Insert

Pten (Pten Mouse)

Use

Lentiviral

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1(-) mouse Egr3

Plasmid

#107998

Purpose

Expresses mouse Egr3 in mammalian cells

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr3 (Egr3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-mTSP33

Plasmid

#236541

Purpose

For expression of Tspan33 in mammalian cells

Depositor

Sandra Citi

Article

Shah et al Cell Rep. 2018 Nov 20;25(8):2132-2147.e7

Insert

TSPAN33 (Tspan33 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

WT

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-E29-ChR2GFP2x

Plasmid

#153437

Purpose

AAV vector to drive the expression of dChr2-GFP-P2A-GFP in PV cortical interneuronsunder the control of the E29 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

Chr2-GFP-P2A-GFP

Use

AAV

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHalo-MyosinIIA-C25

Plasmid

#128573

Purpose

Studies of cellular contractility

Depositor

Dylan Burnette

Article

Fenix et al Elife. 2018 Dec 12;7. pii: 42144. doi: 1

Insert

Myosin IIA (Myh9 Mouse)

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1+FLAG-KrasnatG12D

Plasmid

#206842

Purpose

To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

Depositor

Christopher Counter

Article

Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

Insert

Kras (Kras Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

changed Glycine 12 to Aspartic Acid

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-Dnmt3l

Plasmid

#13356

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

DNA (cytosine-5-)-methyltransferase 3-like (Dnmt3l Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
PKC beta II DN

Plasmid

#16385

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta II (Prkcb Mouse)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (kinase inactive): K371R

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
E407V Nuclear Myosin I YFP

Plasmid

#60242

Purpose

Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

Depositor

Primal de Lanerolle

Article

Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

Insert

Nuclear Myosin 1 (Myo1c Mouse)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

E407V

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
Anti-ATF4 [N360A/24]

Plasmid

#190315

Purpose

Mammalian Expression Plasmid of anti-ATF4 (Human). Derived from hybridoma N360A/24.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-ATF4 (Homo sapiens) recombinant Mouse monoclonal antibody (ATF4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-P(Per2)-DIO-mCherry-Venus-NLS-D2

Plasmid

#110058

Purpose

Per2 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Per2 promoter and mCherry and Venus (Per2 Mouse)

Use

AAV and Cre/Lox

Tags

NLS-D2

Expression

Mammalian

Promoter

Per2

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONOR_MM001-ZFP143-FKBPV-2HA-P2A-GFP-targetting construct

Plasmid

#212705

Purpose

targeting vector to introduce an FKBPV-2HA-P2A-GFP sequence at the C terminal of Zfp143 locus . Degradation tag (dTAG) system

Depositor

Elzo de Wit

Insert

ZFP143-FKBPV-2HA-P2A-GFP

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV UBC msNLRP3(ΔPYD, I125M-END)-APEX2-3xFLAG-IRES-eGFP

Plasmid

#218634

Purpose

Proximity biotinylation of msNLRP3(ΔPYD, I125M-END)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

delPYD (I125M-END)

Promoter

UBC

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-p53P275S

Plasmid

#22726

Depositor

Shinya Yamanaka

Article

Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

Insert

Trp53 (Trp53 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

P275S

Available Since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA

Plasmid

#100279

Purpose

In vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutant

Depositor

Yi Zhang

Article

Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

Insert

Kdm6b (Kdm6b Mouse)

Tags

Flag epitope tag

Expression

Mammalian

Mutation

Mutated catalytic domain (1025aa-End) (please see…

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-nArgBP2ΔNSE

Plasmid

#74515

Purpose

Express GFP-nArgBP2ΔNSE fusion protein in mammalian cells. nArgBP2ΔNSE is the mutant without the neuronal specific exon of nArgBP2.

Depositor

Guoping Feng

Article

Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

Insert

nArgBP2delNSE (Sorbs2 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Cux1

Plasmid

#74341

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Cux1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

cut-like homeobox 1 (Cux1 Mouse)

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLgw Cbx1-V5-EcoDam

Plasmid

#59211

Purpose

Mammalian DamID positive control lentiviral plasmid. Expresses Cbx1-V5-Dam.

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Insert

Cbx1 (Cbx1 Mouse)

Use

Lentiviral; Damid

Tags

Dam and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter and heat shock minima…

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-3xFLAG-Rbfox1

Plasmid

#213725

Purpose

Mammalian cell expression vector for overexpression of Rbfox1 in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

Rbfox1 (Rbfox1 Mouse)

Expression

Mammalian

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PSD-95 scFv [K28/74]

Plasmid

#190490

Purpose

Mammalian Expression of PSD-95 scFV. Derived from hybridoma K28/74.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

PSD-95 (Homo sapiens) recombinant scFV (DLG4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PA-DAD-wt

Plasmid

#53095

Purpose

Expresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.

Depositor

Klaus Hahn, Ronen Zaidel-Bar

Article

Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

Insert

LOV(wt)-6aa-DAD

Tags

6x His and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

L47F and C49S in Venus

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYC3.60-C1

Plasmid

#67899

Purpose

Yellow Cameleon 3.60 in a clontech-style backbone for expression in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

YC3.60

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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