164,577 results

1. p246 eSpCas9_2gRNAs_mH11

   Plasmid

   #164854

   Purpose

   gRNA vector for targeting mouse H11 locus

   Depositor

   Philip Jordan

   Article

   Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

   Insert

   gRNAs for targeting mouse H11 locus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. spNW100

   Plasmid

   #173488

   Purpose

   Expresses spNW100 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spNW100
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   P1049L- please see depositor comments

   Promoter

   T7

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. ORFtag_TetR-SD_Frame1

   Plasmid

   #220981

   Purpose

   ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame1

   Depositor

   Alexander Stark

   Insert

   TetR repressor
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK promoter

   Available Since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-P(Per2)-DIO-intron2-Venus-NLS-D2

   Plasmid

   #110057

   Purpose

   Per2 transcription fluorescence reporter

   Depositor

   Eric Erquan Zhang

   Article

   Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

   Insert

   Per2 promoter and Venus (Per2 Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   NLS-D2

   Expression

   Mammalian

   Mutation

   Promoter

   Per2

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS307

   Plasmid

   #51785

   Purpose

   an integrating (YIp) vector with a LYS2 selectable marker

   Depositor

   David Stillman

   Article

   Eriksson et al Biotechniques. 2004 Feb;36(2):212-3.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS Omomyc

   Plasmid

   #113164

   Purpose

   Omomyc expression in mammalian cells

   Depositor

   Sergio Nasi

   Article

   Soucek et al Oncogene. 1998 Nov 12;17(19):2463-72. do

   Insert

   Omomyc (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E57T, E64I, R70Q, R71N

   Promoter

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti-NLS-miRFP670nano-miRFP670

   Plasmid

   #216131

   Purpose

   Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Keratin 8 (pcDNA3)

   Plasmid

   #18063

   Depositor

   M Bishr Omary

   Article

   Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

   Insert

   Keratin 8 (KRT8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pscALPSpuro-MmACE2 (Mouse)

   Plasmid

   #158087

   Purpose

   Expresses mouse ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Mouse ACE2 (Ace2 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

    Plasmid

    #154868

    Purpose

    Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry

    Depositor

    Ulrik Gether

    Has Service

    AAV Retrograde and AAV8

    Insert

    hM3D(Gq)-mCherry
    

    Use

    AAV; Flp-frt

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin-1

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX856

    Plasmid

    #62888

    Purpose

    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS, NLS SV40, and VP64

    Expression

    Mammalian

    Mutation

    Asparagine 863 to Alanine (N863A)

    Promoter

    CBh

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-C-mStayGold-Zeo

    Plasmid

    #227255

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pm-iATPSnFR1.0

    Plasmid

    #102548

    Purpose

    membrane displayed ATP sensor for mammalian cells

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry

    Plasmid

    #172620

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX855

    Plasmid

    #62887

    Purpose

    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Aspartic acide 10 to Alanine (D10A)

    Promoter

    CBh

    Available Since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMax_ScaI_BFP

    Plasmid

    #177824

    Purpose

    Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    tagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5' inserted ScaI RE cut site

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-VAMP7

    Plasmid

    #45913

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    vesicle-associated membrane protein 7 (Vamp7 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOpen-phi29pol

    Plasmid

    #165573

    Purpose

    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    phi29 DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. tdTomato-H2B-6

    Plasmid

    #58101

    Purpose

    Localization: Nucleus/Histones, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    Dec. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. Gamillus/ pcDNA3

    Plasmid

    #124837

    Purpose

    mammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein

    Depositor

    Takeharu Nagai

    Article

    Shinoda et al Cell Chem Biol. 2018 Mar 15;25(3):330-33

    Insert

    Gamillus
    

    Use

    Tags

    No tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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