4,146 results

1. pAAV-EF1a-FLEX-GTB

   Plasmid

   #26197

   Depositor

   Edward Callaway

   Article

   Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

   Insert

   Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Tags

   Expression

   Mutation

   TVA, rabies glycoprotein are mammalian codon opti…

   Promoter

   Available since

   Nov. 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-SFFV-dCas9-BFP

   Plasmid

   #46910

   Purpose

   Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP

   Depositor

   Jonathan Weissman, Stanley Qi

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9-BFP fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2xNLS, BFP, and HA

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti PGK V5-LUC Neo (w623-2)

   Plasmid

   #21471

   Purpose

   3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Neo

   Depositor

   Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   V5-LUC
   

   Use

   Lentiviral and Luciferase

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. Eps15-pmCherryN1

   Plasmid

   #27696

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   Eps15 (EPS15 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. APEX2-OMM

   Plasmid

   #79056

   Purpose

   Soybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling protein

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   Soybean APEX2
   

   Use

   Lentiviral

   Tags

   flag and last 31 amino acids of human MAVS

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV immearly promotor

   Available since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV hUbC-Cas9-T2A-GFP

   Plasmid

   #53190

   Purpose

   3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized Cas9 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Human SERCA2a (pcDNA3.1+)

   Plasmid

   #75187

   Purpose

   Mammalian expression of ATP2A2

   Depositor

   David MacLennan, Jonathan Lytton

   Article

   Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

   Insert

   ATP2A2 (ATP2A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. VP12

   Plasmid

   #72247

   Purpose

   Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A and Q926A

   Promoter

   CMV

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. MI-Luciferase-IRES-mCherry

   Plasmid

   #75020

   Purpose

   Encodes the expression of Luciferase and mCherry

   Depositor

   Xiaoping Sun

   Article

   Mallampati et al Blood. 2014 Jun 26;123(26):4064-76. doi:

   Inserts

   firefly Luciferase
   mCherry
   

   Use

   Luciferase and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-hACE2

    Plasmid

    #145033

    Purpose

    Express human ACE2 with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    human ACE2 (ACE2 Human)
    

    Use

    Tags

    C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pSBtet-GP

    Plasmid

    #60495

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TCE & RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHM6-alphasynuclein-WT

    Plasmid

    #40824

    Depositor

    David Rubinsztein

    Article

    Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    6x His and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.3_KLF4

    Plasmid

    #26815

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

    Insert

    5'UTR-KLF4-3'UTR (KLF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Starts from second ATG

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pDB1 (CK2alpha)

    Plasmid

    #27083

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2alpha (CSNK2A1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTNT-B18R

    Plasmid

    #58978

    Purpose

    expresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Insert

    B18R
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7/Sp6

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV CreERT2 puro

    Plasmid

    #22776

    Purpose

    Retroviral expression of tamoxifen-inducible Cre-ERT2

    Depositor

    Tyler Jacks

    Article

    Kumar et al Genes Dev. 2009 Dec 1;23(23):2700-4. doi

    Insert

    CreERT2
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    Jan. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Flag-JunWT-Myc

    Plasmid

    #47443

    Purpose

    mammalian expression of WT c-Jun

    Depositor

    Axel Behrens

    Article

    Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi

    Insert

    Jun (Jun Mouse)
    

    Use

    Tags

    Flag and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV5B-Flag-Smurf1 wt

    Plasmid

    #11752

    Depositor

    Jeff Wrana

    Article

    Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

    Insert

    SMURF1 (SMURF1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pFETCh_Donor (EMM0021)

    Plasmid

    #63934

    Purpose

    3X Flag with P2A-Neo for 3' tagging of endogenous proteins

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    Glycine-Serine-3XFlag-P2A-NeoR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Egr1

    Plasmid

    #11729

    Depositor

    Eileen Adamson

    Article

    Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

    Insert

    Egr-1 (Egr1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Flag-Bcl2

    Plasmid

    #18003

    Depositor

    Clark Distelhorst

    Article

    Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

    Insert

    Bcl-2 (BCL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. cytoplasmic EKAR (Cerulean-Venus)

    Plasmid

    #18679

    Depositor

    Karel Svoboda

    Article

    Harvey et al PNAS 2008 Dec 9;105(49):19264-9

    Insert

    ERK Activity Reporter
    

    Use

    Tags

    monomeric Cerulean and monomeric Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-CW3SL-EGFP

    Plasmid

    #61463

    Purpose

    AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAVS1-Pur-CAG-EGFP

    Plasmid

    #80945

    Purpose

    human AAVS1 site targeting donor plasmid for knocking-in EGFP expression cassette driven by CAG promoter

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pB-GLUT4-7myc-GFP

    Plasmid

    #52872

    Purpose

    detection and measurement of GLUT4 translocation

    Depositor

    Jonathan Bogan

    Article

    Bogan et al Mol Cell Biol. 2001 Jul;21(14):4785-806.

    Insert

    GLUT4 (Slc2a4 Rat, with human c-terminus)
    

    Use

    Retroviral

    Tags

    7x-myc (internal) and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGRG25

    Plasmid

    #16665

    Depositor

    Nancy Craig

    Article

    McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

    Insert

    mTn7::MCS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PT3.5/CMV-EGFRvIII

    Plasmid

    #20280

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    EGFRvII (EGFR Human)
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    See depositor comments below.

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYSEAP

    Plasmid

    #37326

    Depositor

    John Schiller

    Article

    Pastrana et al Virology. 2004 Apr 10;321(2):205-16.

    Insert

    SEAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby

    Plasmid

    #71760

    Purpose

    mGFP and Synaptophysin-mRuby expression from Cre-expressing neurons

    Depositor

    Liqun Luo

    Article

    Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

    Has Service

    AAV1

    Inserts

    mGFP
    Synaptophysin
    

    Use

    AAV and Cre/Lox

    Tags

    Palmitoylation signal and mRuby

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AAV-GFP/Cre

    Plasmid

    #49056

    Purpose

    Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.

    Depositor

    Fred Gage

    Article

    Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

    Insert

    Cre
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    EGFP, Myc, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge