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Showing: 2601 - 2640 of 30298 results

  2. pLKO-Tet-puro-hARAF-shRNA-1

    Plasmid
    #185370
    Purpose
    For tetracycline-inducible mammalian expression of shRNA: GCCGTGACCAGATTATCTTTA that targets human ARAF
    Depositor
    Ayaz Najafov
    Insert
    ARAF (ARAF Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185370-plasmid-map-sequence-id-363837

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  11. pPuro3.1(+)_Strep-Lrrk2(delGTPase)-Myc

    Plasmid
    #161587
    Purpose
    expression of Myc-tagged mouse Lrrk2 without GTPase domain
    Depositor
    Maik Hintze
    Insert
    Lrrk2 (Lrrk2 Mouse)
    Use
    Tags
    Myc tag and StrepII
    Expression
    Mammalian
    Mutation
    deletion: AA1023-1879
    Promoter
    CMV
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    161587-plasmid-map-sequence-id-318592

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  12. pPuro3.1(+)_Strep-Lrrk2(delKinase)-Myc

    Plasmid
    #161586
    Purpose
    expression of Myc-tagged mouse Lrrk2 without kinase domain
    Depositor
    Maik Hintze
    Insert
    Lrrk2 (Lrrk2 Mouse)
    Use
    Tags
    Myc tag and StrepII
    Expression
    Mammalian
    Mutation
    deletion: AA1871-2540
    Promoter
    CMV
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    161586-plasmid-map-sequence-id-318591

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  14. pIVTR(T7A)-CRE-T2A-PuroR

    Plasmid
    #172298
    Purpose
    In vitro transcription of CRE-T2A-PuroR synthetic construct for the excision of floxed cassettes
    Depositor
    Patrik Verstreken
    Insert
    CRE-T2A-PuroR
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    T7 promoter A-initiating
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172298-plasmid-map-sequence-id-340323

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  15. Direct-seq_lentiGuide-Puro-Tail-8A8G

    Plasmid
    #157981
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Article
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (Tail-8A8G)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    157981-plasmid-map-sequence-id-309444

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  36. LA601: pMVP (L3-L2) P2A-Puro + WPRE

    Plasmid
    #121785
    Purpose
    pMVP L3-L2 entry plasmid, contains Puro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to a gene in lentivirus vectors
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    P2A-Puro + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121785-plasmid-map-sequence-id-237283

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  38. pAAV-CMV-dSa VP64 Neurog2

    Plasmid
    #99695
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Synthetic, Mouse)
    Use
    AAV and CRISPR
    Tags
    VP64
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Sept. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    99695-plasmid-map-sequence-id-194570

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  39. 3xFN-Tel-TAL/pMXs-puro

    Plasmid
    #63590
    Purpose
    Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector with the puromycin resistance gene.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038
    Insert
    3xFVN-Tel-TAL
    Use
    Retroviral and TALEN
    Tags
    3xFLAG-tag, V5-tag, and nuclear localization sign…
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    63590-plasmid-map-sequence-id-108379

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Showing: 2601 - 2640 of 30298 results