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Plasmid#72556PurposeHypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R/K164R.DepositorInsertp53 (TP53 Human)
UseTags5XHREExpressionMammalianMutationK120R/K164RPromoterE1B minimal promoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lox-stop-Lox p53 R270H
Plasmid#14853DepositorInsertp53 R270H (Trp53 Mouse)
UseCre/LoxTagsLox-STOP-loxExpressionMammalianMutationContact mutant R270H. (Murine p53 codon 270 corre…PromoterAvailable sinceApril 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRRLHygro-pEF1a-p53ashL344P-mKate2-splitmVenusC
Plasmid#69587PurposeExpresses mutated p53-L344P tagged with mKate2 and split C-term mVenusDepositorInsertp53-L344P (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - C termExpressionMammalianMutationp53 L344P mutation that prevents dimerization and…PromoterEF1alphaAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
PG13-gal (wt p53 binding sites)
Plasmid#16446DepositorInsertp53-binding site (TP53 )
UseBeta-gal reporterTagsbeta-galExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-dCas9VPP300-T2A-GFP-shP53
Plasmid#102896PurposeEBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.DepositorInsertdCas9VPP300-T2A-GFP-shP53
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
human p53-(1-393)-M340Q-L344R
Plasmid#24863DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationMethionine 340 mutated to Glutamine Leucine 344 …PromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ash-mKate2-splitmVenusN
Plasmid#69582PurposeExpresses p53 tagged with mKate2 and split N-term mVenusDepositorInsertp53 (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - N termExpressionMammalianMutation7 silent mutations in p53 DNA to prevent targetin…PromoterEF1alphaAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
BDS-2 3x MUT (p53 binding site)
Plasmid#16516DepositorInsertp53 binding site (SFN Human)
UseTagsLuciferaseExpressionMammalianMutationMutant p53 binding sitesPromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28a SUMO Nup53xl 162-267
Plasmid#86002Purposebacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53DepositorTypeEmpty backboneUseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ashL344P-mKate2-splitmVenusN
Plasmid#69586PurposeExpresses mutated p53-L344P tagged with mKate2 and split N-term mVenusDepositorInsertp53-L344P (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - N termExpressionMammalianMutationp53 L344P mutation that prevents dimerization and…PromoterEF1alphaAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
P532_POU4F2-p2A-tdTomato-p2A-Thy1.2
Plasmid#239102PurposeHuman donor plasmid for integration of p2A-h2b-EGFP into the 3' end of POU4F2 (aka BRN3B). This includes the donor casette and a U6 driven guide sequence to be used with Cas9.DepositorInsertPOU4F2 (POU4F2 Human)
UseDonor plasmid for integration into the human geno…Tagsp2A-tdTomato-p2A-Thy1.2ExpressionMammalianMutationPromoterEndogenous POU4F2Available sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR-HRAS(G12V)-IRES-p53DD
Plasmid#233186PurposeGateway entry vector for HRAS(G12V) and p53DD (RIDD)DepositorUseOtherTagsExpressionMutationG12VPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-p53DD-IRES-HRAS(G12V)
Plasmid#233187PurposeRetroviral expression of p53DD and HRAS(G12V) (DDIR)DepositorUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR-p53DD-IRES-HRAS(G12V)
Plasmid#233188PurposeGateway entry vector for p53DD and HRAS(G12V) (DDIR)DepositorUseOtherTagsExpressionMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mCherry-human p53 1-50 (HK180)
Plasmid#206458Purposep53 1-50 expression in Schneider cells for imagingDepositorInsertp53 1-50 (TP53 Human)
UseTagsPH mcherryExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-IRSp53 P278D P281D
Plasmid#221320PurposeDox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transductionDepositorInsertIRSp53 (Baiap2 Mouse)
UseRetroviralTags3xFLAGExpressionMutationP278D P281DPromoterTRE3GAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-IRSp53 with STING pLxIS
Plasmid#221281PurposeExpression of IRSp53 with STING pLxIS (NLV265RLL) in mammalian cells by retroviral transductionDepositorUseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-STING with IRSp53 pLxIS
Plasmid#221282PurposeExpression of STING with IRSp53 pLxIS (RLL361NLV) in mammalian cells by retroviral transductionDepositorUseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-IRSp53(pLxIS) S269A
Plasmid#221269PurposeExpression of IRSp53 pLxIS mutant in mammalian cells by retroviral transductionDepositorInsertIRSp53 pLxIS (Baiap2 Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationS269APromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tev-IntC-P53(304-393)
Plasmid#200315Purposebacterial expression of P53 TETCTD for segmental labelingDepositorInsertp53 (304-393) (TP53 Human)
UseTagsHis6GB1tev and designed intein CExpressionBacterialMutationPromoterT7Available sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-sgTrp53-sgAtrx-EFS-Cre
Plasmid#189977PurposeExpresses Cre cDNA and sgRNAs targeting murine Trp53 and AtrxDepositorInsertsgTrp53, sgAtrx, Cre recombinase
UseAdenoviral, Cre/Lox, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Anti-IRSp53/BAIAP2 [L117/52R]
Plasmid#188156PurposeMammalian Expression Plasmid of anti-IRSp53/BAIAP2 (Human). Derived from hybridoma L117/52DepositorInsertanti-IRSp53/BAIAP2 (Homo sapiens) recombinant mouse monoclonal antibody (BAIAP2 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-WRAP53beta-siRNA-resistant
Plasmid#64680Purposeexpresses siRNA resistant WRAP53betaDepositorInsertWD40-encoding RNA antisense to P53 (WRAP53 Human)
UseTagsFLAGExpressionMammalianMutationsiRNA resistantPromoterCMVAvailable sinceMay 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ashL344A-mKate2-splitmVenusN
Plasmid#69584PurposeExpresses mutated p53-L344A tagged with mKate2 and split N-term mVenusDepositorInsertp53-L344A (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - N termExpressionMammalianMutationp53 L344A mutation that prevents tetramerization …PromoterEF1alphaAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pRRLHygro-pEF1a-p53ashL344A-mKate2-splitmVenusC
Plasmid#69585PurposeExpresses mutated p53-L344A tagged with mKate2 and split C-term mVenusDepositorInsertp53-L344A (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - C termExpressionMammalianMutationp53 L344A mutation that prevents tetramerization …PromoterEF1alphaAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
MG15-luc (mut p53 binding sites)
Plasmid#16443DepositorInsertp53-binding site (mutant) (TP53 )
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-HIF2a dPA (P405A, P531A)
Plasmid#232954PurposeStably express a constitutively stable version of HIF2a (HIF2a dPA)DepositorInsertHIF2a (EPAS1 Human)
UseLentiviralTagsExpressionMammalianMutationP405A, P531APromoterEF1aAvailable sinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
MIP 227 CoMiP 4in1 wo shRNA p53
Plasmid#63727PurposeTo express Oct4, Klf4, Sox2, and c-Myc. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).UseTagsIRES-dtomatoExpressionMammalianMutationcodon-optimisedPromoterSFFVAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
p53 (dominant negative R175H mutant)-pcw107-V5
Plasmid#64638Purpose3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insertDepositorInsertTP53 (transcript variant 3) (TP53 Human)
UseLentiviralTagsV5ExpressionMutationR175HPromoterPGKAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn
Plasmid#214102PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dnDepositorInsertPE-Plus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
MIP 247 CoMiP 4in1 with shRNA p53
Plasmid#63726PurposeTo express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).UseTagsIRES-dtomatoExpressionMammalianMutationcodon-optimisedPromoterSFFV for Pct4, Klf4, Sox2 and c-myc; U6 for p53Available sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)
Plasmid#44027DepositorInsertmHIF-2α (P405A/P530V/N851A) (Epas1 Mouse)
UseTagsMycExpressionMammalianMutationP405A, P530V, N851APromoterCMVAvailable sinceMarch 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
NSK024 SFFV-mCherry-SG(p53R248Q.s2)SG-EGFP (FLP-IN)
Plasmid#191169PurposeExpression of a sensor for p53 mutant R248Q in mammalian cells; mCherry marker, EGFP outputDepositorInsertmCherry:p53(R248Q).s2:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-p53 gRNA-Cas9-T2A-mCherry
Plasmid#164256PurposeTranscription of p53 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cellsDepositorInsertsg p53
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-3*Flag-APEX2 N-term WRAP53
Plasmid#187578PurposeExpress FLAG epitope and APEX2-tagged WRAP53 fusion protein in mammalian cellsDepositorInsertWRAP53 (WRAP53 Human)
UseTagsAPEX2-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A;P531A;bHLH*-pBabe-Puro
Plasmid#22135DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids from wt: Proline 405 t…PromoterAvailable sinceNov. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A/P531A/N847A-pBabe-Puro
Plasmid#25956DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…PromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A/P531A/N847Q-pBabe-Puro
Plasmid#25948DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…PromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
MG15-gal (mut p53 binding sites)
Plasmid#16447DepositorInsertp53-binding site (mutant) (TP53 )
UseBeta-gal reporterTagsbeta-galExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
MSCV FLIPi P2G_Thy1.1 Dbl (p53,PTEN)
Plasmid#19746DepositorInsertoligo targeting p53 and PTEN (Pten Mouse)
UseCre/Lox, RNAi, and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMXs-HRAS(G12V)-IRES-SNAP-p53DD
Plasmid#233200PurposeRetroviral expression of HRAS(G12V) and SNAP-p53DDDepositorUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-SNAP-p53DD-IRES-HRAS(G12V)
Plasmid#233198PurposeRetroviral expression of SNAP-p53DD and HRAS(G12V)DepositorUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR-SNAP-p53DD-IRES-HRAS(G12V)
Plasmid#233201PurposeGateway entry vector for SNAP-p53DD and HRAS(G12V)DepositorUseOtherTagsExpressionMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFUW-SNAP-p53DD-IRES-HRAS(G12V)
Plasmid#233206PurposeLentiviral dox-inducible expression of SNAP-p53DD and HRAS(G12V)DepositorUseLentiviralTagsExpressionMammalianMutationG12VPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR-HRAS(G12V)-IRES-SNAP-p53DD
Plasmid#233199PurposeGateway entry vector for HRAS(G12V) and SNAP-p53DDDepositorUseOtherTagsExpressionMutationG12VPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-IRSp53-T2A-Rac1(G12V)
Plasmid#221330PurposeDox-inducible expression of FLAG-IRSp53-T2A-HA-Rac1 G12V in mammalian cells by retroviral transductionDepositorUseRetroviralTagsExpressionMutationG12VPromoterTRE3GAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-IRSp53-T2A-Cdc42(G12V)
Plasmid#221329PurposeDox-inducible expression of FLAG-IRSp53-T2A-HA-Cdc42 G12V in mammalian cells by retroviral transductionDepositorUseRetroviralTagsExpressionMutationG12VPromoterTRE3GAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-STING L373A with IRSp53 pLxIS
Plasmid#221283PurposeExpression of STING L373A with IRSp53 pLxIS (RLL361NLV) in mammalian cells by retroviral transductionDepositorUseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGW02 AAV-TBG-Cre-sgP53-sgRNA
Plasmid#192162PurposeAAV-TBG-Cre-sgP53-sgRNADepositorTypeEmpty backboneUseAAVTagsExpressionMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
MLRV3:HRE-P53-MAPK/JNK-FOXO-TGFβ
Plasmid#178320PurposeMultiplex luciferase reporter vector, with luciferase reporters for the pathways HRE, P53, AP-1, FOXO and SMAD.DepositorInsertsHRE RedFirefly reporter
P53 FLuc reporter
AP-1 Renilla reporter
FOXO NLuc Reporter
SMAD GrRenilla reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only