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We narrowed to 11,682 results for: nts

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Showing: 121 - 140 of 11682 results

  1. pUCmini-iCAP-AAV.CAP-B10

    Plasmid
    #175004
    Purpose
    non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system
    Depositor
    Viviana Gradinaru
    Article
    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do
    Insert
    Synthetic construct isolate AAV.CAP-B10 VP1 gene
    Use
    AAV
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available Since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    175004-plasmid-map-sequence-id-347131

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  2. pAAV-hSyn-hM3D(Gq)-mCherry

    Plasmid
    #50474
    Purpose
    Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM3D(Gq)-mCherry (CHRM3 Human)
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50474-plasmid-map-sequence-id-471606

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  5. pLenti puro HA-Ubiquitin

    Plasmid
    #74218
    Purpose
    Lentiviral expression of HA tagged Ubiquitin
    Depositor
    Melina Fan
    Insert
    ubiquitin (UBB Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Single ubiquitin coding sequence (full length UBB…
    Promoter
    CMV
    Available Since
    March 14, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    74218-plasmid-map-sequence-id-148715

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  6. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid
    #140003
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140003-plasmid-map-sequence-id-337354

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  8. dxCas(3.7)-VPR

    Plasmid
    #108383
    Purpose
    Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus
    Depositor
    David Liu
    Article
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Insert
    dxCas(3.7)-VPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Available Since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    108383-plasmid-map-sequence-id-207172

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  16. CMV-LUC2CP/ARE

    Plasmid
    #62857
    Purpose
    Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Article
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Insert
    Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    destabilizing sequences (DS) added to the C termi…
    Promoter
    CMV
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    62857-plasmid-map-sequence-id-212900

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  17. pKSE401

    Plasmid
    #62202
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    35S and AtU6-26p
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62202-plasmid-map-sequence-id-288285

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  18. pAAV-EF1a-DIO-hM4D(Gi)-mCherry

    Plasmid
    #50461
    Purpose
    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter
    Depositor
    Bryan Roth
    Insert
    hM4D(Gi)-mCherry (CHRM4 Human)
    Use
    AAV
    Tags
    HA and mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    EF1a
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50461-plasmid-map-sequence-id-182308

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  19. pYD1

    Plasmid
    #73447
    Purpose
    expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells
    Depositor
    Dane Wittrup
    Article
    Kieke et al Protein Eng. 1997 Nov;10(11):1303-10.
    Type
    Empty backbone
    Tags
    6xHis, AGA2, T7 peptide, V5 tag, and Xpress tag
    Expression
    Yeast
    Promoter
    GAL1
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    73447-plasmid-map-sequence-id-364550

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Showing: 121 - 140 of 11682 results