-
Plasmid#53220Purpose{Ud} is composed of two genes; (1) a UAS-RpL14.dsRNA targeting RNAi to a haploinsufficient gene RpL14 and (2) an RNAi insensitive RpL14 rescueDepositorInsertRpL14 (RpL14 Fly)
UseAttb drosophila melanogaster transformationTagsExpressionMutation14 synonymous mutations introducedPromoterendogenous promoterAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJPC063
Plasmid#234533PurposeTRV2 expressing Int13DepositorInsertIntegrase 13 recombinase
UseRecombinaseTagsExpressionMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJPC062
Plasmid#234534PurposeTRV2 expressing CinHDepositorInsertCinH Recombinase
UseRecombinaseTagsExpressionMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJPC060
Plasmid#234535PurposeTRV2 expressing FLPeDepositorInsertFLPe recombinase
UseRecombinaseTagsExpressionMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
XLone-BSD NFIL3 T2A Puro
Plasmid#140028PurposeTunable and temporal expression control of NFIL3 and puro resistant geneDepositorInsertNFIL3 (NFIL3 Synthetic, Human)
UseSynthetic BiologyTagspuromycinExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS
Plasmid#132927PurposepMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInserthumanized SpCas9
UseCRISPR and Synthetic Biology; Pmagic gateway entr…TagsExpressionMutationPromoterAvailable sinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS_nanorod_105b
Plasmid#120347Purposephagemid containing all components for assembly of a DNA origami nanorod (10-helix bundle)DepositorInsertStaple sequences for a DNA origami nanorod
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-WGA-mCherry
Plasmid#224119PurposeTo express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.DepositorInsertWGA-mCherry
UseAAVTagsWGA and mCherryExpressionMammalianMutationAll mutations related to the WGA in this construc…PromoterCAGIGAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSLR
Plasmid#51500PurposeSingle component plant T-DNA plasmid. Contains cis- and trans-acting replicational elements from the bean yellow dwarf virus in an LIR-SIR-Rep/RepA-LIR orientation.DepositorTypeEmpty backboneUsePlant t-dna plasmidTagsExpressionMutationPromoterAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro NFIL3 P2A eGFP NLS
Plasmid#140026PurposeTunable and temporal expression control of NFIL3 and nuclear eGFPDepositorInsertNFIL3 and enhanced Green Fluorescent Protein (NFIL3 Synthetic, Human)
UseSynthetic BiologyTagseGFPExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDSG320
Plasmid#115597Purposesurface-displayed Nb2, used in composability experiments; medium copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG318
Plasmid#115595Purposesurface-displayed Ag2, used in composability experiments; medium copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG321
Plasmid#115598Purposesurface-displayed Nb3, used in composability experiments; medium copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG289
Plasmid#115608Purposesurface-displayed Nb2, used in composability experiments; low copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG319
Plasmid#115596Purposesurface-displayed Ag3, used in composability experiments; medium copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG287
Plasmid#115606Purposesurface-displayed Ag2, used in composability experiments; low copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG291
Plasmid#115605Purposesurface-displayed Null, used in composability experiments; low copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG290
Plasmid#115609Purposesurface-displayed Nb3, used in composability experiments; low copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG288
Plasmid#115607Purposesurface-displayed Ag3, used in composability experiments; low copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH04_32_sRNA-A_Sp_coD
Plasmid#120900Purposethe component plasmid of a regulated deviceDepositorInsertspECF32 promoter [i.e., Pecf32_1122 (-60 to -1)]
sRNA-A
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Zdk3-VPR
Plasmid#231572PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertZdk3-VPR, Ef1a driven
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
Zdk3-VP64
Plasmid#231571PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertZdk3-VP64
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_Zdk3-VPR
Plasmid#231573PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertZdk3-VPR, CMV driven
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLX208-beta-arrestin2-HA-TEV219
Plasmid#104846Purposeb2AR-SPARK protease componentDepositorInsertbeta-arrestin2-HA-TEV219
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
XLone-GFP
Plasmid#96930PurposeTunable and temporal expression control of GFPDepositorInsertGreen Fluorescent Protein
UseTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV:eGFP-p2A-HA-β2Ar-uTEV1Δ(220-242)
Plasmid#135459PurposeMammalian expression of SPARK protease componentDepositorInserteGFP-p2A-HA-βarrestin2-uTEV1Δ
UseLentiviralTagseGFP, HAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
XLone-BSD SARS-CoV2 N P2A mCherry
Plasmid#154398PurposePiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and mCherryDepositorInsertSARS-CoV2 N Protein (N SARS-CoV2)
UsePiggybacTagsmCherryExpressionMammalianMutationPromoterTRE3GAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET24c(+)-mtEFTu
Plasmid#30535DepositorInsertMature bovine mitochondrial elongation factor Tu
UseTagsHisExpressionBacterialMutationMature form amino acids 44-452. Mito import seque…PromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
CMV:β2Ar-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5
Plasmid#135458PurposeMammalian expression of SPARK TF componentDepositorInsertβ2AR-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5
UseLentiviralTagsFlag, V5ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
apt-ABE8.20
Plasmid#219938PurposeMammalian expression of apt-ABE8.20 protein (TadA8.20_32aa-linker_Com)DepositorInsertTadA8.20_32-aa_Com
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNUT1
Plasmid#175746PurposeSingle-component photo-inducible (LOV2-based) nucleolus-targeting tool 1DepositorInsertmCherry-LOV2-NoLS (HIV Rev transactivating protein)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJPC055
Plasmid#234536PurposeTRV2 expressing CreDepositorInsertCre recombinase
UseRecombinaseTagsExpressionMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Gal4-pcVP16-1
Plasmid#231570PurposeExpresses the single component, light-off regulated pcVP16-1 transcriptional regulatorDepositorInsertGal4-pcVP16-1
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
cpLOV2-Gal4
Plasmid#231569PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertcpLOV2-Gal4
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
-
pCAG-NLS-HA-Dre
Plasmid#51272PurposeComponent of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)DepositorInsertNLS-HA-Dre
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS-HA-Bxb1
Plasmid#51271PurposeComponent of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)DepositorInsertNLS-HA-Bxb1
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M13-TEV-C-P2A-TdTomato
Plasmid#92389Purposeexpresses Cal-light M13-TEV-C component and TdTomato in mammalian cellsDepositorInsertM13-TEV-C-P2A-TdTomato
UseTagsTdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLSLGFP.R
Plasmid#51501PurposeSingle component LSL vector with GFP. Rep is expressed from the complementary sense LIR promoter. GFP is between LIR and SIR and is driven from a 2X35S promoter. Rep is within the repliconDepositorInsertGFP
UsePlant t-dna plasmidTagsExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp Phy-mCherry-CAAX
Plasmid#50839PurposeOptogenetic PI3K activation system; membrane componentDepositorInsertPhyB 1-908
UseLentiviral and Synthetic BiologyTagsmCherryExpressionMutationPromoterSFFVAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only