Addgene

We narrowed to 4,075 results for: PRS

Showing: 3501 - 3550 of 4075 results

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piHGpd/T101I

Plasmid

#1112

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

HSP82 with threonine 101 changed to isoleucine; r…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-D260N

Plasmid

#10985

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-D260N (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 778 G to A in the RPB1 ORF resulting i…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-D261N

Plasmid

#10986

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-D261N (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 781 G to A in the RPB1 ORF resulting i…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-R320C

Plasmid

#10987

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-R320C (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 958 C to T in the RPB1 ORF resulting i…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-E1230K

Plasmid

#10990

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-E1230K (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 3688 G to A in the RPB1 ORF resulting in…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pYO411

Plasmid

#235732

Purpose

Protein expression of ZZ-FL yeast Atg14 in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

ATG14

Use

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO831

Plasmid

#235754

Purpose

Protein expression of ZZ-ScVPS38 (1-212F)

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Use

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

aa 1-212F

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO225

Plasmid

#235729

Purpose

Protin expression of FL yeast VPS15 -ZZ in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS15

Use

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO290

Plasmid

#235731

Purpose

Protein expression in FL yeast VPS34 D731N mutant in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Use

Tags

Expression

Yeast

Mutation

S2A, D731N

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO69

Plasmid

#235747

Purpose

Protein expression of FL ScVPS34 in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Use

Tags

Expression

Yeast

Mutation

S2A

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO361

Plasmid

#235748

Purpose

Protein expression of ScVPS34 HELCAT

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 HELCAT

Use

Tags

Expression

Yeast

Mutation

aa 268-875

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO227

Plasmid

#235749

Purpose

Protein expression of ZZ-FL ScVPS30

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS30

Use

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO832

Plasmid

#235755

Purpose

Protein expression of ScVPS30 (1-215E), untagged

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

ScVPS30

Use

Tags

Expression

Yeast

Mutation

aa 1-215E

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO478

Plasmid

#235757

Purpose

Expression of FL ScVPS34-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Use

Tags

3xFLAG

Expression

Yeast

Mutation

Promoter

ScVPS34 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO764

Plasmid

#235761

Purpose

Expression of ScVPS38-EGFP under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Use

Tags

EGFP

Expression

Yeast

Mutation

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PAF23

Plasmid

#223171

Purpose

Expresses yeGFP under control of CTR1 promoter and terminator

Depositor

Ryan Peterson

Article

Anne et al J Fungi (Basel). 2024 Oct 21;10(10):729.

Insert

green fluorescent protein

Use

Tags

Expression

Yeast

Mutation

Promoter

Saccharomyces cerevisiae CTR1

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PAF24

Plasmid

#223172

Purpose

Expresses VC83_00191-yeGFP under control of CTR1 promoter and terminator

Depositor

Ryan Peterson

Article

Anne et al J Fungi (Basel). 2024 Oct 21;10(10):729.

Insert

Copper Transporter

Use

Tags

StreptagII and yeGFP

Expression

Yeast

Mutation

Promoter

saccharomyces cerevisiae CTR1

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SIR4 gRNA HIS

Plasmid

#182520

Purpose

Expression vector for gRNA directed against SIR4 ORF.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

SIR4 gRNA (SIR4 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CAN1 gRNA HIS

Plasmid

#182521

Purpose

Expression vector for gRNA directed against CAN1 ORF.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

CAN1 gRNA (CAN1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
csr-1_son-1_sGFP

Plasmid

#191561

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Use

Tags

NCU04502 promoter and sGFP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
csr-1_son-1_mRuby3

Plasmid

#191744

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Use

Tags

NCU04502 promoter and mRuby3

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
csr-1_son-1_mNeptune2.5

Plasmid

#191748

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Use

Tags

NCU04502 promoter and mNeptune2.5

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLS.601

Plasmid

#184955

Purpose

Express -Eco1 ncRNA library variants

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 ncRNA (variants)

Use

Tags

Expression

Bacterial

Mutation

Library members with mutations described in manus…

Promoter

T7/lac

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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PALI1_PIR_pFB1

Plasmid

#191249

Purpose

Expresses PALI1 from insect cells

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

LCoR

Use

Tags

MBP

Expression

Insect

Mutation

NA

Promoter

Polyhedrin

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Yeast_Target-AID2S

Plasmid

#188647

Purpose

Expressing Target-AID2S in yeasts

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nCas9-AID2S-UGI

Use

Tags

UGI

Expression

Yeast

Mutation

D10A for SpCas9

Promoter

pGal1

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC_N_PAX2

Plasmid

#187736

Purpose

MAC-tagged gene expression of human PAX2

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

PAX2 (PAX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAF4E9D

Plasmid

#185844

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>LacI>TUP1>PABF1

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAF9C3

Plasmid

#185843

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>RevTetR_r1.7>SIN3C>PABF1

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAF9D91

Plasmid

#185845

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>TetR>SIN3C>PABF1

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFZifPZ432

Plasmid

#185850

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>ZifPZ43>MED15>PAFB1

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAF9D93

Plasmid

#185847

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>TetR>MIG1C>PABF1

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAF9D92

Plasmid

#185846

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>TetR>TUP1>PABF1

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KBB520

Plasmid

#185102

Purpose

1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP

Depositor

Ken Belanger

Article

Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

Insert

POM152

Use

Tags

GFP

Expression

Yeast

Mutation

POM152(1-200)-GFP

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KBB521

Plasmid

#185103

Purpose

1st 960 amino acids (C-terminal truncation) of Pom152 fused with HA and GFP

Depositor

Ken Belanger

Article

Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

Insert

POM152

Use

Tags

GFP

Expression

Yeast

Mutation

POM152(1-960)-HA-GFP

Promoter

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KBB329

Plasmid

#185077

Purpose

NUP82 expression in yeast

Depositor

Ken Belanger

Article

Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

Insert

NUP82

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KBB280

Plasmid

#185073

Purpose

Larger fusion of Nma111 nuclear localization signal to GFP

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Use

Tags

GFP

Expression

Yeast

Mutation

NMA111 aa 1-83

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KBB382

Plasmid

#185085

Purpose

HA-tagged POM152 with glycosylation sites mutated

Depositor

Ken Belanger

Article

Belanger et al Genetics. 2005 Nov;171(3):935-47. doi: 1

Insert

POM152

Use

Tags

HA

Expression

Yeast

Mutation

Pom152 N280A, N398A, N???A, N???A = Pom152Dglyc

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only
pAF7E1

Plasmid

#185839

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>Zif268>MED15>PABF1

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAF4EH4R

Plasmid

#185837

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>Zif268>VP16-PABF1

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KBB282

Plasmid

#185074

Purpose

Smaller fusion of Nma111 nuclear localization signal to GFP

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Use

Tags

GFP

Expression

Yeast

Mutation

NMA111 aa 1-35

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KBB465

Plasmid

#185098

Purpose

NMA111-GFP with NLS1 deleted under endogenous promoter

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Use

Tags

GFP

Expression

Yeast

Mutation

NMA111DNLS1-GFP

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KBB466

Plasmid

#185099

Purpose

NMA111-GFP with NLS1 and NLS2 deleted under endogenous promoter

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Use

Tags

GFP

Expression

Yeast

Mutation

NMA111DNLS1DNLS2-GFP

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KBB467

Plasmid

#185100

Purpose

NMA111-GFP with NLS2 deleted under endogenous promoter

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Use

Tags

GFP

Expression

Yeast

Mutation

NMA111DNLS2-GFP

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDK038

Plasmid

#134182

Purpose

Expression of C. elegans KNL1

Depositor

Iain Cheeseman

Article

Kern et al Mol Biol Cell. 2015 Jan 15;26(2):229-37.

Insert

KNL1 aa 69-235 (knl-1 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSADuet148

Plasmid

#121910

Purpose

Charge-introduced NpuDnaBΔ283ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

CI-NpuDnaBΔ290ΔC39 intein

Use

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBDuet259

Plasmid

#121911

Purpose

NpuDnaBΔ283ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

NpuDnaBΔ283ΔC39 intein

Use

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 283-residue endonuclease domain

Promoter

T7

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBDuet116

Plasmid

#121915

Purpose

NpuDnaBΔ290ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

Oth-NpuDnaBΔ290ΔC39 intein

Use

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0317

Plasmid

#128979

Purpose

Encodes Zif268DBD-CRY2 under pscADH1

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pscADH-SV40NLS-Zif268DBD-CRY2 (L3)-tscADH1 scTRP1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0320

Plasmid

#128980

Purpose

Encodes Zif268DBD-CRY2PHR under pscADH1

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pscADH1-SV40NLS-ZIF268DBD-CRY2PHR (L3)-tscADH1 TRP1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0521

Plasmid

#128984

Purpose

Expression plasmid for ZIF268DBD-L3-CRY2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

SV40NLS-Zif268DBD-CRY2 (L3) scTRP1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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