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Showing: 3501 - 3550 of 4899 results

  21. NK809

    Plasmid
    #196406
    Purpose
    Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Tags
    Expression
    Mutation
    N/A (synthetic)
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196406-plasmid-map-sequence-id-385017

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  22. NK810

    Plasmid
    #196407
    Purpose
    Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Tags
    Expression
    Mutation
    N/A (synthetic)
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196407-plasmid-map-sequence-id-385018

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  23. NK811

    Plasmid
    #196408
    Purpose
    Nanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly control
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Tags
    Expression
    Mutation
    N/A (synthetic)
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196408-plasmid-map-sequence-id-384995

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  25. p5’UTR-ORF1

    Plasmid
    #190566
    Purpose
    Holding vector containing the contiguous 5’UTR and ORF1 regions of human L1 (L1.3) to facilitate subcloning of ORF1 mutants into the full-length L1 reporter pRTC2-puro (Cook et al., 2015).
    Depositor
    Anthony Furano
    Article
    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112
    Insert
    Contiguous regions of the human L1 (L1.3) 5’UTR and ORF1
    Use
    For dna propagation only
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    N/A
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    190566-plasmid-map-sequence-id-393237

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  31. pILGFP4D42

    Plasmid
    #185840
    Purpose
    Testing the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185840-plasmid-map-sequence-id-369622

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  33. pILGFP10CF71

    Plasmid
    #185860
    Purpose
    Testing the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185860-plasmid-map-sequence-id-370799

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  35. pILGFP10CF72

    Plasmid
    #185857
    Purpose
    Testing the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185857-plasmid-map-sequence-id-370796

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  36. pILGFP10E1B

    Plasmid
    #185856
    Purpose
    Testing the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185856-plasmid-map-sequence-id-370794

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  37. pILGFP10E1A

    Plasmid
    #185855
    Purpose
    Testing the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185855-plasmid-map-sequence-id-370793

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  38. pILGFP3A2 (1)

    Plasmid
    #185851
    Purpose
    Testing the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185851-plasmid-map-sequence-id-370760

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  39. pTS2341_Tier3(SB)-Puro

    Plasmid
    #169639
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    169639-plasmid-map-sequence-id-366789

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  40. pTS1041

    Plasmid
    #169619
    Purpose
    Tier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven expression of secreted Nluc and mTagBFP2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PPGK
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    169619-plasmid-map-sequence-id-366634

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  41. pVH1001-Tier2(ColE1 ori)

    Plasmid
    #169599
    Purpose
    Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and ColE1 ori).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169599-plasmid-map-sequence-id-366207

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  42. pTS2351_Tier3(SB)-Zeo

    Plasmid
    #169648
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a ZeoR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-ZeoR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven ZeoR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169648-plasmid-map-sequence-id-366198

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  43. pTS1040

    Plasmid
    #169618
    Purpose
    Tier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven expression of secreted Nluc and mTagBFP2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PPGK
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169618-plasmid-map-sequence-id-365617

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Showing: 3501 - 3550 of 4899 results