We narrowed to 4,881 results for: phy

1. pAAV-CMV-SauriCas9

   Plasmid

   #135964

   Purpose

   Expresses SauriCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGL2-NOS2Promoter-Luciferase

   Plasmid

   #19296

   Depositor

   Charles Lowenstein

   Article

   Lowenstein et al Proc Natl Acad Sci U S A. 1993 Oct 15. 9

   Insert

   NOS2 Promoter (Nos2 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. -2.5Kb MMP10

   Plasmid

   #53973

   Purpose

   Luciferase reporter for mouse MMP10 2.5kb promoter

   Depositor

   Michael Chin

   Article

   Wu et al Biochem Biophys Res Commun. 2011 Dec 2;4

   Insert

   mouse MMP10 2.5Kb promoter (Mmp10 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PCR from C57BL6/J

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-TDsmURFP-IRES-HO-1

   Plasmid

   #80346

   Purpose

   Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   Tandem Dimer smURFP
   HO-1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and IRES

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAEMXT-eLACCO1.1

   Plasmid

   #167946

   Purpose

   Fluorescent biosensor for extracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

   Insert

   CD59-eLACCO1.1-GPI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. IK349

   Plasmid

   #233655

   Purpose

   Dox-inducible red light-inducible Flp-C; Flp-397C-PhyA

   Depositor

   Wilson Wong

   Article

   Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

   Insert

   Flp-397C-PhyA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   TRE3G

   Available since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcdna nesprin TS

   Plasmid

   #68127

   Purpose

   nesprin tension sensor

   Depositor

   Daniel Conway

   Article

   Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

   Insert

   mouse nesprin mini 2G (Syne2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV2-GCaMP6f-P2A-H2B-PAmCherry

   Plasmid

   #133419

   Purpose

   Fluorescent reporter for calcium imaging and photoactivation

   Depositor

   Tim Holy

   Article

   Lee et al Science. 2019 Dec 13;366(6471):1384-1389

   Insert

   GCaMP6f-P2A-H2B-PAmCherry (H2BC11 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDR111_Bgal no NLS

   Plasmid

   #188397

   Purpose

   Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

   Depositor

   Christopher Contag

   Article

   Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

   Insert

   lacZ
   

   Use

   Tags

   PhoD secretion peptide

   Expression

   Bacterial

   Mutation

   Promoter

   Phyper spank

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CMV-Sa-SauriCas9

    Plasmid

    #135967

    Purpose

    Expresses Sa-SauriCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TDP-43 mRuby

    Plasmid

    #216225

    Purpose

    TDP-43 with a C-terminal mRuby tag for expression in mammalian cells.

    Depositor

    Jennifer Lee

    Article

    Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

    Plasmid

    #163076

    Purpose

    Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    mito-roGFP2-Orp1
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAT.000

    Plasmid

    #119559

    Purpose

    Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified BioBrick vector, RSF1010-derived ori.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ZapLck biosensor

    Plasmid

    #131584

    Purpose

    Lck FRET Biosensor. To visualize Lck kinase activity in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Wan et al Sci Adv. 2019 Jun 19;5(6):eaau2001. doi:

    Insert

    ZapLck biosensor
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-hSyn-eLACCO1.1

    Plasmid

    #168788

    Purpose

    Neuronal expression of fluorescent sensor for extracellular lactate

    Depositor

    Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    eLACCO1.1
    

    Use

    AAV

    Tags

    ER export and GPI

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-GFAP-eLACCO1.1

    Plasmid

    #168790

    Purpose

    Astrocyte expression of fluorescent sensor for extracellular lactate

    Depositor

    Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    eLacco1.1
    

    Use

    AAV

    Tags

    ER export and GPI

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBiFC-bJunVN173

    Plasmid

    #22012

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    bJunVN173 (Jun Rat, Aequorea Victoria)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    c-Jun(257-338)-Venus(1-172)

    Promoter

    Available since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPML1 (GB0045)

    Plasmid

    #68164

    Purpose

    Provides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtML1 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CAG-eLACCO1.1

    Plasmid

    #168789

    Purpose

    Fluorescent sensor for extracellular lactate

    Depositor

    Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    eLACCO1.1
    

    Use

    AAV

    Tags

    ER export and GPI

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQdC12a

    Plasmid

    #191356

    Purpose

    Clostridium CRISPRi plasmid with TetR-repressible dCas12a (LbCpf1) expression

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Inserts

    PGusA2-TetO2/1-dCas12a (LbCpf1)
    miniPthl-tetR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PGus2A-TetO2/1 and miniPthl

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. linker-mCitrine-PIF6-NAT

    Plasmid

    #51576

    Purpose

    Tag protein of interest with PIF6 in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PIF6 (PIL2 Mustard Weed)
    

    Use

    Tags

    linker and mCitrine

    Expression

    Yeast

    Mutation

    1-100aa of PIF6

    Promoter

    none

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CAG-SaCas9-WPRE

    Plasmid

    #89996

    Purpose

    Expresses high levels of WT SaCas9 for genome editing

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

    Insert

    SaCas9 (NEWENTRY Staphylococcus aureus)
    

    Use

    Tags

    3xHA, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGDP3 apmA

    Plasmid

    #112881

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    apmA (apmA Staphylococcus aureus)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGDP4 ermC

    Plasmid

    #112884

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    ermC (ermM Staphylococcus epidermis)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDB179-TfCut2-S121P-D174S-D204P

    Plasmid

    #218695

    Purpose

    Encodes codon optimized TfCut2-S121P-D174S-D204P enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    TfCut2
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    S121P-D174S-D204P

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mEos2-FilaminA-N-9

    Plasmid

    #57379

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    FilaminA (FLNA Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    D2626H

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

27. pcdna nesprin HL

    Plasmid

    #68128

    Purpose

    nesprin headless control

    Depositor

    Daniel Conway

    Article

    Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

    Insert

    nesprin-2 (Syne2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted actin-binding domain

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTYF-super-mfPENK-0.9k-Venus-WPRE

    Plasmid

    #156401

    Purpose

    Expresses Venus under PENK gene promoter

    Depositor

    Shuji Kaneko

    Article

    Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

    Inserts

    Venus
    Gal4p65
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMAH-POLY-eRF1(E55D)

    Plasmid

    #157925

    Purpose

    Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

    Depositor

    Huiwang Ai

    Article

    Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

    Insert

    POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a-HsSENP2

    Plasmid

    #71466

    Purpose

    Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 2 (SENP2 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

31. pMOS012: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

    Plasmid

    #163057

    Purpose

    Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Grx1-roGFP1-iEER
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. SLF045: AAV-hSyn-eTsChR-GFP

    Plasmid

    #112280

    Purpose

    AAV based broad expression of eTsChR in neurons

    Depositor

    Adam Cohen

    Article

    Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

    Insert

    eTsChR
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1-TRPV1exCellHalo

    Plasmid

    #220307

    Purpose

    Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices

    Depositor

    Eric Senning

    Article

    Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231

    Insert

    rTRPV1exCellHalo (Trpv1 Rat)
    

    Use

    Tags

    circularly permutated Halotag (Deo et al., Nat Ch…

    Expression

    Mammalian

    Mutation

    linker included to insert cpHaloTag (

    Promoter

    CMV promoter

    Available since

    July 30, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pEGB 35S:Luciferase:Tnos (GB0110)

    Plasmid

    #68216

    Purpose

    Transcriptional unit for Firefly Luciferase Protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-synp-F-H2B-jRGECO1a

    Plasmid

    #102995

    Purpose

    Expresses histone fused jRGECO1a under the synapsin promoter

    Depositor

    David Cox

    Article

    Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

    Insert

    H2B-jRGECO1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CTV

    Plasmid

    #15912

    Depositor

    Klaus Rajewsky

    Article

    Thai et al Science. 2007 Apr 27. 316(5824):604-8.

    Insert

    CAG-STOP-eGFP-ROSA26TV
    

    Use

    Cre/Lox; /stop

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEGFP-C1-KSENB-Nup133

    Plasmid

    #87340

    Purpose

    To express human Nup133 in mammalian cells

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP133 (NUP133 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deletion of Nup133 AA1-7

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTYF-super-mfSST-0.3k-Venus-WPRE

    Plasmid

    #156393

    Purpose

    Expresses Venus under SST gene promoter

    Depositor

    Shuji Kaneko

    Article

    Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

    Inserts

    Venus
    Gal4p65
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28a-HsSENP1

    Plasmid

    #71465

    Purpose

    Encodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 1 (SENP1 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

40. pEGB 35S:Renilla:Tnos (GB0109)

    Plasmid

    #68215

    Purpose

    Transcriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Renilla
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge