We narrowed to 5,937 results for: plasmid dna
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Plasmid#109053PurposehU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromoterhU6Available SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only
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AV09_pDonor.EP.S1.TS
Plasmid#100297PurposeAAVS1-targeting expression plasmidDepositorInsertPuroR.T2A.EGFP expression unit
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMBP-LbCas12a
Plasmid#113431PurposeExpresses 10xHis-MBP fused to LbCas12aDepositorHas ServiceCloning Grade DNAInsertLbCas12a
TagsMBPExpressionBacterialPromoterT7Available SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA-CBh-mStayGold
Plasmid#241379PurposeBxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CBh promoterDepositorInsertmStayGold
ExpressionMammalianPromoterCBhAvailable SinceSept. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-Ku80
Plasmid#46958PurposePlasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.DepositorAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
gRNA_Cloning Vector
Plasmid#41824PurposeAn empty gRNA expression vector, used to create a gRNA to a specific sequence.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsAflII site for Gibson AssemblyExpressionMammalianAvailable SinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSUP-(2xCouRS-tRNAopt)
Plasmid#197930PurposeMachinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycineDepositorInsertsCouRS
tRNA opt
ExpressionBacterialPromoterglnS' and lambda(ts) and proKAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-modified
Plasmid#84477Purposeguide RNA expression lentiviral constructDepositorInsertguide RNA
UseLentiviralExpressionMammalianPromotermU6Available SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-Ku70
Plasmid#46957PurposePlasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.DepositorAvailable SinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-scFvGCN4sfGFPTET1CD
Plasmid#82561PurposeExpression vector for antibody-sfGFP-TET1CD.DepositorInsertscFvGCN4sfGFPTET1CD
ExpressionMammalianAvailable SinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI3M-TBD
Plasmid#113077PurposeExpresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI3M-TBD
ExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCap-101-Apr
Plasmid#227507PurposeCapture plasmid with apramycin resistance and pIJ101 repliconDepositorTypeEmpty backboneAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CK8e_Cas9_miniPA
Plasmid#226141PurposeAAV construct for HITI insert with CK8e promoter and SpCas9DepositorInsertCas9
UseAAV and CRISPRExpressionMammalianPromoterCK8eAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI5M
Plasmid#113078PurposeExpresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
UseCRISPRExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRel
Plasmid#227581PurposeRelease plasmid with Cas9 working cassette, apramycin resistance, and pSG5 repliconDepositorInsertCas9
UseCRISPRAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCap-101-Hgy
Plasmid#227508PurposeCapture plasmid with hygromycin resistance and pIJ101 repliconDepositorTypeEmpty backboneAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRel-Hyg
Plasmid#227582PurposeRelease plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 repliconDepositorInsertCas9
UseCRISPRAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-TetOn_mScarlet_Divergent
Plasmid#229791PurposeBxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expressionDepositorInsertTRE-mScarlet; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsNLSExpressionMammalianPromoterTRE and CAGAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHelp
Plasmid#227583PurposeAccessory plasmid with a sgRNA cassette for Cas9, and ampicillin resistanceDepositorInsertguide RNA scaffold
UseCloning vectorAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT-CAG-mStayGold
Plasmid#241387PurposeBxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CAG promoterDepositorInsertmStayGold
ExpressionMammalianPromoterCAGAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_AIO-TetOn_Divergent Donor
Plasmid#229784PurposeBxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)DepositorInsertLacZ; rtTA-T2A-mTagBFP2
UseSynthetic BiologyExpressionMammalianPromoterTRE and CAGAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-TetOn_Divergent Donor
Plasmid#229786PurposeBxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)DepositorInsertLacZ; rtTA-T2A-mTagBFP2
UseSynthetic BiologyExpressionMammalianPromoterTRE and CAGAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMBP-SpyCatcher
Plasmid#79053PurposeExpresses maltose binding protein (MBP) SpyCatcher fusionDepositorInsertMBP-SpyCatcher
Tags6xHis and Maltose binding proteinExpressionBacterialMutationDerived from pDEST14-SpyCatcher (Plasmid #35044)…PromoterT7Available SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
rs214-minor
Plasmid#113380Purposeevaluate FOXA1 DIV motif enhancer activity in SNP rs2149943 minor allele (T)DepositorInsertrs2149943 minor allele
UseLuciferaseAvailable SinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCmEos3.2:cat
Plasmid#78100PurposePlasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.DepositorInsertmEos3.2-cat
ExpressionBacterialAvailable SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_CAG_3xTurboGFP-NLS
Plasmid#229777PurposeBxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP (3 copies)DepositorInsert3x TurboGFP
UseSynthetic BiologyTagsNLSExpressionMammalianPromoterCAGAvailable SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_CAG-TurboGFP
Plasmid#229781PurposeBxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFPDepositorInsertTurboGFP
UseSynthetic BiologyTagsNLSExpressionMammalianPromoterCAGAvailable SinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
AX53_pS.Donor.S1.TS
Plasmid#100290PurposeAAVS1-targeting plasmidDepositorInsert18 bp RFLP (MluI+KspAI)
UseCRISPR and Synthetic Biology; Human targetingExpressionMammalianAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKB01-PssbB-mKate2
Plasmid#69105PurposeExpresses mKate2 (codon adapted for Streptococcus pneumoniae); under control of CSP inducible promoter PssbB. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.DepositorInsertmKate2
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLPC31
Plasmid#209996PurposeContains Level 0 Part: 3' Connector (3C2SN) for the construction of Level 1 plasmidsDepositorInsert3' Connector (3C2SN)
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC32
Plasmid#209997PurposeContains Level 0 Part: 3' Connector (3C5CSN) for the construction of Level 1 plasmidsDepositorInsert3' Connector (3C5CSN)
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC52
Plasmid#209991PurposeContains Level 0 Part: 5' Connector (5C2SN) for the construction of Level 1 plasmidsDepositorInsert5' Connector (5C2SN)
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC51
Plasmid#209990PurposeContains Level 0 Part: 5' Connector (5C1CSN) for the construction of Level 1 plasmidsDepositorInsert5' Connector (5C1CSN)
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLuciferase (GB0096)
Plasmid#68201PurposeProvides the CDS of the firefly luciferase protein as a level 0 GoldenBraid partDepositorInsertLuciferase coding region
UseLuciferase and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
AY42_pS.Donor.R5
Plasmid#100291PurposeCCR5-targeting plasmidDepositorInsert18 bp RFLP (MluI+KspAI)
UseCRISPR and Synthetic Biology; Human targetingExpressionMammalianAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAF256
Plasmid#105496PurposepRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and LgBiTDepositorInsertHupA-SmBiT/LgBiT
UseAtc-dependent expression in c. difficilePromoterPtetAvailable SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAF257
Plasmid#105497PurposepRPF185 derived plasmid encoding an anhydrotetracyclin inducible SmBiT and HupA-LgBiTDepositorInsertSmBiT/HupA-LgBiT
UseAtc-dependent expression in c. difficilePromoterPtetAvailable SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRenilla (GB0056)
Plasmid#68198PurposeProvides the CDS of the Renilla protein as a level 0 GoldenBraid partDepositorInsertRenilla coding region
UseLuciferase and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA0055
Plasmid#131774PurposeGuide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.DepositorInsert20mer CASS5a guide (gCASS5a) and 5' sgRNA
ExpressionBacterial and YeastAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only