Skip to main content
Addgene
Showing: 3601 - 3650 of 13557 results

  3. pAAV-CMV-FLEX-TVAmCherry-2A-oG

    Plasmid
    #102985
    Purpose
    AAV expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG) after CRE-mediated recombination
    Depositor
    Marco Tripodi
    Article
    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306
    Inserts
    TVA-mCherry fusion protein after CRE-mediated recombination
    Optimised Rabies Glycoprotein (oG) after CRE-mediated recombination
    Use
    AAV; Adeno associated viral vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    102985-plasmid-map-sequence-id-199982

    Enlarge

  5. pTUB1:OsTIR1-3FLAG, SAG1:CAT

    Plasmid
    #87258
    Purpose
    TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.
    Depositor
    David Sibley
    Article
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    TIR1
    CAT
    Use
    Toxoplasma expression
    Tags
    3FLAG
    Expression
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgSAG1 361 bp and TgTUB1 2717 bp
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87258-plasmid-map-sequence-id-176556

    Enlarge

  8. pYSD313

    Plasmid
    #212931
    Purpose
    Encodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    Sed1 (SED1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212931-plasmid-map-sequence-id-421768

    Enlarge

  15. p415 TEF roGFP2‐Tsa2ΔCPΔCR

    Plasmid
    #83239
    Purpose
    The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.
    Depositor
    Tobias Dick
    Article
    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do
    Insert
    roGFP2-Tsa2dCpdCr
    Use
    Tags
    roGFP2 fusion with Tsa2dCpdCr
    Expression
    Yeast
    Mutation
    Promoter
    TEF
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    83239-plasmid-map-sequence-id-158262

    Enlarge

  16. MP9956:SFG.GD3synthase-2A-GD2synthase

    Plasmid
    #75013
    Purpose
    Retroviral transfer vector which encodes the Disialoganglioside (GD2) mini-operon. Mammalian cells transduced with this vector become GD2 positive.
    Depositor
    Martin Pule
    Article
    Thomas et al PLoS One. 2016 Mar 31;11(3):e0152196. do
    Inserts
    murine GD3 synthase
    murine GD2 synthase
    Use
    Retroviral; Momlv retroviral vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    75013-plasmid-map-sequence-id-141876

    Enlarge

  18. pDIV301

    Plasmid
    #204969
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and ble (bleomycin) resistance marker
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    ble (bleomycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    204969-plasmid-map-sequence-id-454125

    Enlarge

  28. pAluYa5-neo-TET

    Plasmid
    #51283
    Purpose
    expresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Article
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    AluYa5
    Use
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Mutation
    Promoter
    upstream pol III enhancer sequence of the 7SL gen…
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    51283-plasmid-map-sequence-id-78362

    Enlarge

  30. GFP-LiMETER-v1

    Plasmid
    #113933
    Purpose
    Express an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFP
    Depositor
    Yubin Zhou
    Article
    Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.
    Insert
    LOV2 and Rit polybasic domain (PB)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    113933-plasmid-map-sequence-id-225305

    Enlarge

  34. pDule2-Acd82

    Plasmid
    #174100
    Purpose
    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
    Depositor
    Ryan Mehl
    Article
    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.
    Insert
    Acridone 82 tRNA synthetase and tRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N311S, C313G, V366A, W382T
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    174100-plasmid-map-sequence-id-345717

    Enlarge

  46. pclbw-eGFPomp25

    Plasmid
    #58424
    Purpose
    Mammalian expression of GFP targeted to the mitochondrial outer membrane
    Depositor
    David Chan
    Article
    Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi
    Insert
    eGFP-omp25 (Synj2bp Mouse)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    contains aa's 109-145 of mouse omp25
    Promoter
    CMV/b-actin
    Available since
    Dec. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  48. pAAV-shCTRL

    Plasmid
    #181875
    Purpose
    Expresses a control shRNA with no known targets in the mouse genome under control of the U6 promoter and mCherry under control of the CaMKIIa promoter
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Insert
    control shRNA with no known targets in the mouse genome
    Use
    AAV, Adenoviral, and Mouse Targeting
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181875-plasmid-map-sequence-id-355955

    Enlarge

  50. pJH4203

    Plasmid
    #200160
    Purpose
    Prig-3 FRT let858 (stop) FRT twk-40(gf)::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40 (gf ) GFP
    Depositor
    Mei Zhen
    Article
    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do
    Insert
    twk-40 (twk-40 Nematode)
    Use
    Tags
    GFP
    Expression
    Worm
    Mutation
    Promoter
    Prig-3
    Available since
    May 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    200160-plasmid-map-sequence-id-413719

    Enlarge

Showing: 3601 - 3650 of 13557 results