We narrowed to 44,084 results for: cha

1. pCR1165

   Plasmid

   #111111

   Purpose

   Expresses HELQ_-_84376979.23-P1P2 sgRNA in pCR1068

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   HELQ gRNA (HELQ Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCR1082

   Plasmid

   #111102

   Purpose

   Expresses FANCE_-_35420187.23-P1P2 sgRNA in pCR1068

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   FANCE gRNA (FANCE Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCR1135

   Plasmid

   #111103

   Purpose

   Expresses FANCE_-_35420147.23-P1P2 sgRNA in pCR1068

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   FANCE gRNA (FANCE Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUDE724

   Plasmid

   #103023

   Purpose

   expression of a Cpf1 programming crRNA targetting PDR12 (crPDR12-3S)

   Depositor

   Jean-Marc Daran

   Article

   Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

   Insert

   crPDR12-3.S (PDR12 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENTR-2B-Nkx2-5 Y191C

   Plasmid

   #98613

   Purpose

   Gateway entry vector for NKX2-5 Y191C

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   NKX2-5 Y191C (Nkx2-5 Mouse)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Point mutation Y191C

   Promoter

   Available Since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGL3-IRES-miR34a-P6

   Plasmid

   #64790

   Purpose

   Luciferase expression driven by miR-34a promoter P6

   Depositor

   Joshua Mendell

   Article

   Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

   Insert

   miR-34a promoter P6 (MIR34A Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGL3-IRES-miR34a-P3

   Plasmid

   #64787

   Purpose

   Luciferase expression driven by miR-34a promoter P3

   Depositor

   Joshua Mendell

   Article

   Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

   Insert

   miR-34a promoter P3 (MIR34A Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL3-IRES-miR34a-P5

   Plasmid

   #64789

   Purpose

   Luciferase expression driven by miR-34a promoter P5

   Depositor

   Joshua Mendell

   Article

   Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

   Insert

   miR-34a promoter P5 (MIR34A Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-IRES-miR34a-P4

   Plasmid

   #64788

   Purpose

   Luciferase expression driven by miR-34a promoter P4

   Depositor

   Joshua Mendell

   Article

   Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

   Insert

   miR-34a promoter P4 (MIR34A Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBABE-puro-THAP11-rescue-3xFLAG

    Plasmid

    #36998

    Depositor

    Debu Chakravarti

    Article

    Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

    Insert

    THAP11 (THAP11 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Mutations were generated at codons 273-275 conver…

    Promoter

    Available Since

    May 21, 2012

    Availability

    Academic Institutions and Nonprofits only

11. dCas9-KRAB-MeCP2

    Plasmid

    #110821

    Purpose

    Improved dCas9 repressor-dCas9-KRAB-MeCP2

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pB-CAGGS-dCas9-KRAB

    Plasmid

    #110822

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 2C-EGFP-t2a-mCD4

    Plasmid

    #216213

    Purpose

    Expresses GFP and mCD4 transmembrane domain under control of the 2C-specific MERVL promoter

    Depositor

    Michelle Percharde

    Article

    Xie et al Genes Dev. 2022 Mar 1;36(5-6):331-347. d

    Insert

    CD4 (Cd4 Mouse)
    

    Use

    Tags

    GFP-T2A

    Expression

    Mammalian

    Mutation

    CD4 transmembrane domain only

    Promoter

    MERVL

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. dCas9-KRAB

    Plasmid

    #110820

    Purpose

    Conventional dCas9 repressor-dCas9-KRAB

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJT305 Pa01 Lenti pEF-attB LSR GFP

    Plasmid

    #184948

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Ami Bhatt, Michael Bassik, Lacra Bintu, Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV-SFFV-RTN3-WPRE-UbC-Emerald

    Plasmid

    #233467

    Purpose

    Lentiviral vector plasmid expressing human reticulon 3 (RTN3) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Evan Reid, Bart Nieuwenhuis, Richard Eva

    Inserts

    RTN3 (RTN3 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJT301 BxBi Lenti pEF-attB LSR GFP

    Plasmid

    #184944

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Ami Bhatt, Michael Bassik, Lacra Bintu, Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    BxBi Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDF-His-mTET1CD

    Plasmid

    #81053

    Purpose

    bacterial expression of the catalytic domain of murine TET1

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TET1 (Tet1 Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    catalytic domain amino acid 1367-2057

    Promoter

    T7

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pB-CAGGS-dCas9

    Plasmid

    #110823

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJT306 Kp03 Lenti pEF-attB LSR GFP

    Plasmid

    #184949

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Ami Bhatt, Michael Bassik, Lacra Bintu, Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLV-CAG-F14F15S-Switch

    Plasmid

    #99733

    Purpose

    Flp-based FLEX reporter (from RFP to GFP)

    Depositor

    Ian Wickersham

    Article

    Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

    Insert

    TagRFP and EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCEP4-TAPBPR-TM

    Plasmid

    #178645

    Purpose

    Mammalian expression of FLAG-tagged TAPBPR with MHC TM

    Depositor

    Erik Procko

    Article

    McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

    Insert

    TAPBPR (TAPBPL Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from HLA class I …

    Expression

    Mammalian

    Mutation

    switched transmembrane domain with that of MHC-I

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCR8/GW/TOPO-ERF

    Plasmid

    #98626

    Purpose

    Gateway entry TOPO TA cloning vector ERF

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ERF (Erf Mouse)
    

    Use

    Topo ta cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJT302 Ec03 Lenti pEF-attB LSR GFP

    Plasmid

    #184945

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Ami Bhatt, Michael Bassik, Lacra Bintu, Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Ec03 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJT303 Ec04 Lenti pEF-attB LSR GFP

    Plasmid

    #184946

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Ami Bhatt, Michael Bassik, Lacra Bintu, Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Ec04 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJT304 Ec07 Lenti pEF-attB LSR GFP

    Plasmid

    #184947

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Ami Bhatt, Michael Bassik, Lacra Bintu, Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Ec07 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTET-IUPi

    Plasmid

    #122019

    Purpose

    Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.

    Depositor

    Gregory Stephanopoulos

    Article

    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

    Inserts

    Choline kinase (CKI1 Budding Yeast)
    Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
    isopentenyl-diphosphate Delta-isomerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pTET

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUDE710

    Plasmid

    #103020

    Purpose

    expression of a Cpf1 programming crRNA targeting ADE2 and HIS4 (crADE2-3 crHIS4-4.S)

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    crADE2-3.crHIS4-4.S (ADE2, HIS4 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pENTR-2B-Gata4

    Plasmid

    #98615

    Purpose

    Gateway entry vector for Gata4

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    Gata4 (Gata4 Mouse)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLgw EcoDam-V5-SRF

    Plasmid

    #98602

    Purpose

    Mammalian DamID lentiviral vector forSRF with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    SRF (Srf Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCR8/GW/TOPO-ELK1

    Plasmid

    #98616

    Purpose

    Gateway entry TOPO TA cloning vector for ELK1

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ELK1 (Elk1 Mouse)
    

    Use

    Topo ta cloning vector

    Tags

    Expression

    Mutation

    Q230R

    Promoter

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pENTR-2B-Nkx2-5 delta HD-G8

    Plasmid

    #98614

    Purpose

    Gateway entry vector NKX2-5 _HD

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    NKX2-5 _HD (Nkx2-5 Mouse)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Deletion of the homeodomain. Replaced by 8 glycin…

    Promoter

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGWB515_35S-HA-(ΔTP)DM3(Col-0) D332A Q345A N349A

    Plasmid

    #229716

    Purpose

    Stable transformation or transient expression of gene of interest in plants

    Depositor

    Eunyoung Chae

    Article

    Wan et al Molecular Cell, Volume 85, Issue 14

    Insert

    DM3(Col-0) (AT3G61540 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    amino acid 1-52 were deleted, D332A Q345A N349A

    Promoter

    Available Since

    July 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. FLAG-GRP78-T453D

    Plasmid

    #235209

    Purpose

    Expresses GRP78 with T453D mutation in mammalian cells

    Depositor

    Amy Shiu Lee

    Article

    Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Change Threonine 453 to Aspartic Acid

    Promoter

    Available Since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. FLAG-GRP78-R197H

    Plasmid

    #235208

    Purpose

    Expresses GRP78 with R197H mutation in mammalian cells

    Depositor

    Amy Shiu Lee

    Article

    Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Change Arginine 197 to Histidine

    Promoter

    Available Since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. FLAG-GRP78-G227D

    Plasmid

    #235207

    Purpose

    Expresses GRP78 with G227D mutation in mammalian cells

    Depositor

    Amy Shiu Lee

    Article

    Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Change Glycine 227 to Aspartic Acid

    Promoter

    Available Since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMXs-3xFlag-SPNS1

    Plasmid

    #198568

    Purpose

    SPNS1 codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1 with n-terminal 3xFlag (SPNS1 Human)
    

    Use

    Retroviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pENTR-2B-Nkx2-5YRD Y-A

    Plasmid

    #98611

    Purpose

    Gateway entry vector for NKX2-5 YRD Y-A

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    NKX2-5 YRD Y-A (Nkx2-5 Mouse)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Substitutions of 7 tyr into Ala residues.Y233A; Y…

    Promoter

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pB-CAGGS-dCas9-KRAB-MeCP2

    Plasmid

    #110824

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-hSyn-ASAP3-WPRE

    Plasmid

    #132331

    Purpose

    expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Lenti-AcGFP-Sec61-IRES-Blast

    Plasmid

    #154009

    Purpose

    Lentiviral vector plasmid for integration and constitutive expression of AcGFP fused to Sec61 in mammalian cells – gift from D. Gerlich (IMBA, Vienna)

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    acGFP-Sec61 (SEC61B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Integrin Beta1-mPA-GFP

    Plasmid

    #186302

    Purpose

    Expresses mPA-GFP tagged Integrin beta-1 in mammalian cells on a CMV promoter

    Depositor

    Pakorn Kanchanawong

    Article

    Jain et al Nat Protoc. 2022 Jul 27. pii: 10.1038/s4

    Insert

    Integrin Beta 1 (ITGB1 Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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43. pcDNA 3.1 SIRT1-FL

    Plasmid

    #105670

    Purpose

    Mammalian expression construct of mouse SIRT1 full length isoform

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    CMV

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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44. pAG414GPD-dCas9-VPR

    Plasmid

    #63801

    Purpose

    pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available Since

    April 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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45. pGMC00035 (aka pMG0784)

    Plasmid

    #210768

    Purpose

    Lentiviral vector to express human ADRM1

    Depositor

    Raj Chari

    Article

    Osei-Amponsa et al Mol Cell. 2024 Feb 1;84(3):522-537.e8. d

    Insert

    ADRM1 (ADRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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46. Cav2.1 HA pcDNA3

    Plasmid

    #206069

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. pPICZc-ScAct1*-thymosinB-8His

    Plasmid

    #111148

    Purpose

    Expresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37

    Depositor

    Mohan Balasubramanian

    Article

    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

    Insert

    Act1 (ACT1 Budding Yeast)
    

    Use

    Pichia pastoris integration plasmid

    Tags

    Thymosin beta and a His-tag

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. pAAV-camk2-Sthk-p2a-bpac(WT) minWPRE

    Plasmid

    #118274

    Purpose

    Expresses the SthK channel & bPAC(WT)-mCherry under a minimal CamKII promoter

    Depositor

    Dietmar Schmitz

    Article

    Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

    Inserts

    bPAC(WT)
    SthK
    

    Use

    AAV

    Tags

    His tag, P2A, mCherry, and myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CamK II

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. pMXs-IRES-blast GFP-SPNS1

    Plasmid

    #198571

    Purpose

    SPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1 with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
    

    Use

    Retroviral

    Tags

    3xFlag-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCR8/GW/TOPO-ELK4

    Plasmid

    #98617

    Purpose

    Gateway entry TOPO TA cloning vector for ELK4

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ELK4 (Elk4 Mouse)
    

    Use

    Topo ta cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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