We narrowed to 6,273 results for: nls
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Plasmid#42234DepositorInsertCas9
UseCRISPRTagsGFP, HA epitope, and NLS (nuclear localization si…ExpressionMammalianMutationcodon-optimized synthetic DNA sequencePromoterCMVAvailable SinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTPGI_dCas9_VP64
Plasmid#49013Purposeencodes yeast-optimized dCas9_VP64 synthetic transcription factorDepositorInsertdCas9_VP64 (codon-optimized for expression in S. cerevisiae)
UseCRISPR and Synthetic BiologyTagsSV40 NLSExpressionYeastPromoterpTPGI (galactose+aTc inducible)Available SinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIVT-Bxb1
Plasmid#232746PurposeIVT template for making modRNA encoding Bxb1DepositorInsertBxb1
UseIn vitro transcriptionTagsHA and SV40 NLSAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VCre
Plasmid#89575PurposeExpresses VCre under pCAGDepositorInsertVCre recombinase
UseCre/Lox and Synthetic BiologyTagsNLSExpressionMammalianAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICH47742:FCP:Cas9YFP
Plasmid#85986PurposeGolden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoterDepositorInsertCas9
UseCRISPR; Golden gate assemblyTagsSV40 NLS, YFPPromoterFCPAvailable SinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
M-ST1cas
Plasmid#48669PurposeMammalian S. thermophilus #1 Cas9 expression, human optimizedDepositorInsertCas9
UseCRISPRTagsNLSPromoterCMVAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
Thy1-Brainbow-2.1 R
Plasmid#18727DepositorInserthrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
UseCre/Lox and Mouse TargetingExpressionMammalianAvailable SinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-NED
Plasmid#109358PurposeMammalian vector for expressing effector domains fused to dCas9.DepositorTypeEmpty backboneUseCRISPRTagsdCas9, SV40 NLS, 3xFLAGExpressionMammalianPromoterCMVAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
M-NMcas
Plasmid#48670PurposeMammalian N. meningitidis Cas9 expression, human optimizedDepositorInsertCas9
UseCRISPRTagsNLSPromoterCMVAvailable SinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV_dCas9_VP64
Plasmid#49015Purposeencodes human-optimized dCas9_VP64 synthetic transcription factorDepositorInsertdCas9_VP64 (human-codon-optimized)
UseCRISPR and Synthetic BiologyTagsSV40 NLSExpressionMammalianPromoterCMVAvailable SinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-P2A-mScarlet (MNW002)
Plasmid#174137PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarletDepositorInserthuman codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationSpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…PromoterCMV and T7Available SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL358
Plasmid#169147PurposeEncodes OptoQ-AMP1DepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL513
Plasmid#169153PurposeEncodes OptoQ-INVRT1DepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
mini-donor - NKX2.5-nEGFP
Plasmid#209287Purposethis mini-donor knockin vector can be used to knock nuclear EGFP into NKX2.5 locusDepositorInsertEGFP-nls
UseKnockin donor vectorAvailable SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pTEF1p-CYC1t
Plasmid#62190PurposeExpresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionYeastMutationhuman codon-optimized, D10A, H840APromoterTEF1 promoterAvailable SinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL1394
Plasmid#129522PurposepDEF-SpCas9::GFP, For deletion of pyuis4, PyBiP-minimal promoter, 3rd GenerationDepositorInsertSpCas9
UsePlasmodium, rodent-infectious speciesTags3XFLAG-NLSAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAL728
Plasmid#169152PurposeEncodes OptoQ-AMP6DepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastMutationA79QAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR1056
Plasmid#71824PurposeExpress Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
UseCRISPRAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
M-SPcas
Plasmid#48668PurposeMammalian S. pyogenes Cas9 expression, human optimizedDepositorInsertCas9
UseCRISPRTagsNLSPromoterCMVAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMAL498
Plasmid#169154PurposeEncodes OptoQ-INVRT4DepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR1054
Plasmid#71822PurposeExpress Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
UseCRISPRAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
rtTA and TagBFP cassettes
Plasmid#62452PurposeMXS_chaining vector with 2 cassettesDepositorInsertsCMV::NLS-TagBFPx3-bGHpA
CMV::rtTA3-bGHpA
UseSynthetic BiologyAvailable SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMAL724
Plasmid#169151PurposeEncodes OptoQ-AMP5DepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastMutationA79QAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)
Plasmid#174140PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarletDepositorInserthuman codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationSpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…PromoterCMV and T7Available SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL669
Plasmid#169148PurposeEncodes OptoQ-AMP2DepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL722
Plasmid#169149PurposeEncodes OptoQ-AMP3DepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastMutationA79QAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL716
Plasmid#169150PurposeEncodes OptoQ-AMP4DepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLSLZ.D.R
Plasmid#51510PurposeSingle component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR.DepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant t-dna plasmidTagsNLSPromoter2x35SAvailable SinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCR1055
Plasmid#71823PurposeExpress Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
UseCRISPRAvailable SinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFTK056
Plasmid#171328PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertSpCas9-NLS
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK Smad3NL Flag
Plasmid#14828DepositorAvailable SinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCNPpo6-H98A
Plasmid#46087DepositorInsertPpoI H98A
TagsNLSExpressionMammalianMutationcatalytically inactive H98A variantPromoterCMVAvailable SinceJune 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZLSLD.R
Plasmid#51511PurposeSingle component LSL vector. Contains 2x35S:Zif268:FokI outside of the replicon borders. Within the replicon is the us:NPTII template.DepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant t-dna plasmidTagsNLSPromoter2x35SAvailable SinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLSLGUS
Plasmid#51517PurposepLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmidDepositorInsertsGUS
3kb filler sequence
UsePlant t-dna plasmidTagsNLS and flagPromotervirion-sense LIR and 2x35SAvailable SinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSL1166
Plasmid#129520PurposepDEF-SpCas9::GFP, For deletion of pyalba4, PyBiP-full promoter, 2nd GenerationDepositorInsertSpCas9
UsePlasmodium, rodent-infectious speciesTags3XFLAG-NLSAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFTK047
Plasmid#171319PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertDsRed-NLS fluorescent reporter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK044
Plasmid#171316PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInserteGFP-NLS fluorescent reporter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtinGW
Plasmid#133377PurposePiggybac vector for constitutive expressionDepositorTypeEmpty backboneTagsFlagExpressionMammalianPromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFTK074
Plasmid#171346PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertVPR -NLS AD (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK071
Plasmid#171343PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInserteGPF-NLS fluorescent reporter (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK070
Plasmid#171342PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInserteYFP-NLS fluorescent reporter (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK069
Plasmid#171341PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInserteGFP-NLS fluorescent reporter (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK057
Plasmid#171329PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertdSpCas9(m4)-VPR-NLS
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS42K_PCP-VP64_B-PP7
Plasmid#177157PurposeExpresses transactivator (PCP-VP64) and scRNA (B-PP7) in yeast cellsDepositorInsertsPCP-VP64
B-PP7
TagsSV40 NLSExpressionYeastPromoterADH1 promoter and SNR52 promoterAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
MS2-ecDHFR
Plasmid#65859PurposeExpresses RNA-binding protein MS2 with eDHFR fusion tagDepositorAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL1103
Plasmid#129526PurposepDEF-SpCas9, No Target Control Vector for CRISPRi, PbEF1a promoter, 1st GenerationDepositorInsertdSpCas9
UsePlasmodium, rodent-infectious speciesTagsNLS, 1xHAAvailable SinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL1315
Plasmid#129527PurposepDEF-SpCas9, Best (-458) Vector for CRISPRi of PyALBA4, PbEF1a promoter, 1st GenerationDepositorInsertdSpCas9
UsePlasmodium, rodent-infectious speciesTagsNLS, 1xHAAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
p35SZ
Plasmid#51513PurposepMDC32 with Zif268:FokI followed by approximately 3kb of filler sequenceDepositorInsertsZif268:FokI
3kb filler sequence
UsePlant expression t-dnaTagsNLSPromoter2x35SAvailable SinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLSLZ.D
Plasmid#51496PurposepLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmidDepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant t-dna plasmidTagsSV40 NLSPromotervirion-sense LIR and 2x35SAvailable SinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-intron
Plasmid#241900PurposeLbCas12a-intron Gateway entry plasmidDepositorInsertLbCas12a-intron
UseCRISPRTagsNLSExpressionPlantAvailable SinceDec. 15, 2025AvailabilityAcademic Institutions and Nonprofits only