We narrowed to 33,255 results for: IND

1. pC1-HyPerRed-mito

   Plasmid

   #60247

   Purpose

   Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

   Insert

   HyPerRed-mito
   

   Use

   Tags

   Mitochondrial targeting signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. dead Sa Cas9

   Plasmid

   #138162

   Purpose

   For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   dSaCas9
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   D10A + N580A

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

   Plasmid

   #45574

   Purpose

   Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   co I-Sce I
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. LOTUS-V/pcDNA3

   Plasmid

   #87127

   Purpose

   Bioluminescent and ratiometric voltage indicator

   Depositor

   Takeharu Nagai

   Article

   Inagaki et al Sci Rep. 2017 Feb 13;7:42398. doi: 10.10

   Insert

   LOTUS-V
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCT5-bac2.0

   Plasmid

   #119872

   Purpose

   Cumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

   Depositor

   Claudia Schmidt-dannert

   Article

   Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

   Inserts

   CymR
   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pveg promoter with CuO sequence and PxylR promoter

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti6.2-ccdB-3xFLAG-V5

   Plasmid

   #87071

   Purpose

   Lentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

   Depositor

   Mikko Taipale, Susan Lindquist

   Article

   Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAVS1-Puro Xlone-eGFP

   Plasmid

   #136936

   Purpose

   donor plasmid for inducible transgene expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

   Insert

   all-in-one tet-on system
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS

   Available Since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLSC-5

   Plasmid

   #62889

   Purpose

   Lenti vector for expression of inducible split Cas9

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Inserts

   SpCas9(574-1368)
   SpCas9(2-573)
   

   Use

   CRISPR and Lentiviral

   Tags

   FFBP12, FRB, NES PTK2, NLS SV40, and P2A

   Expression

   Mammalian

   Mutation

   *see comment below

   Promoter

   EFS

   Available Since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC1-mitoRexYFP

   Plasmid

   #60246

   Purpose

   Mitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

   Depositor

   Vsevolod Belousov

   Article

   Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

   Insert

   mitoRexYFP
   

   Use

   Tags

   Mitochondrial targeting signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-hSyn1-FLEX-CybGam-WPRE

    Plasmid

    #207662

    Purpose

    Control for CybSEP2

    Depositor

    Sung Han

    Article

    Kim et al bioRxiv 2023.01.19.524797

    Insert

    Gamillus
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Histidine 86 and 159 to alanines, inserted gamill…

    Promoter

    hSynapsin

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX330-LMNA-gRNA1

    Plasmid

    #122507

    Purpose

    expresses WT spCas9 and a chimeric gRNA targeting human LMNA

    Depositor

    Graham Dellaire

    Article

    Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

    Insert

    LMNA-gRNA1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p426 103Q GAL

    Plasmid

    #1188

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 103Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pTalpha1-iCre

    Plasmid

    #133924

    Purpose

    Neuron-specific expression of iCre

    Depositor

    Sebastián Dupraz, Frank Bradke

    Article

    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

    Insert

    iCre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized

    Promoter

    neuron-specific tubulin-alpha-1 promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAD-GINKO1

    Plasmid

    #113111

    Purpose

    Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    GINKO1
    

    Use

    Tags

    6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQE30-roGFP1-iR

    Plasmid

    #83314

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

    Insert

    roGFP1-iR
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    ro1(C48S/S147C/Q204C) plus X insertion, iR betwe…

    Promoter

    T5

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

16. PB-TRE-EGFP-EF1a-rtTA

    Plasmid

    #104454

    Purpose

    PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

    Depositor

    Volker Busskamp

    Article

    Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

    Inserts

    enhanced green fluorescent protein
    reverse tetracycline-controlled transactivator 3
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTet-IRES-EGFP

    Plasmid

    #64238

    Purpose

    Lentiviral, doxycycline-inducible system to express transgene of interest and EGFP.

    Depositor

    Maria Lung

    Article

    Shuen et al Cancer Gene Ther. 2015 Feb 27. doi: 10.1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTet

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SGTA-EGFP

    Plasmid

    #210373

    Purpose

    Expresses SGTA fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SGTA (SGTA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-TA-ERP2

    Plasmid

    #80477

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmScarlet_peroxisome_C1

    Plasmid

    #85063

    Purpose

    In vivo visualization of the peroxisome (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Peroxisomal targeting sequence
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PB-TA-ERN

    Plasmid

    #80474

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-GINKO1

    Plasmid

    #113112

    Purpose

    Mammalian expression of genetically encoded green fluorescent potassium ion indicator GINKO1

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    GINKO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MSCV-mNINJ1-GFP

    Plasmid

    #208776

    Purpose

    MSCV vector expressing WT mouse NINJ1

    Depositor

    Petr Broz

    Article

    Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

    Insert

    NINJ1 (Ninj1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1/Puro-CAG-JEDI-1P

    Plasmid

    #202607

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P, expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    JEDI-1P
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFvL106

    Plasmid

    #64751

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, URA selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP

    Plasmid

    #232800

    Purpose

    Helper plasmid to trigger auxin-inducible-degradation of targeted proteins.

    Depositor

    Chunliang Li

    Article

    Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

    Insert

    OsTIR1 F74G EGFP-AID
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pmScarlet-i_C1

    Plasmid

    #85044

    Purpose

    Mammalian expression of mScarlet-i

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    mScarlet-i
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP-TRAIL

    Plasmid

    #10953

    Depositor

    Bingliang Fang

    Article

    Kagawa et al Cancer Res. 2001 Apr 15. 61(8):3330-8.

    Insert

    Tumor necrosis factor alpha related apoptosis inducing ligand (TNFSF10 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ER-RpHLuorin2

    Plasmid

    #171717

    Purpose

    Luminal ER expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 targeted to ER
    

    Use

    Tags

    KDEL ER-retention signal and Signal sequence of h…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti6.2-3xFLAG-V5-ccdB

    Plasmid

    #87072

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

    Depositor

    Mikko Taipale, Susan Lindquist

    Article

    Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-TRE-eYFP

    Plasmid

    #104056

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein eYFP

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. LRCherry2.1

    Plasmid

    #108099

    Purpose

    Lentiviral expression plasmid of sgRNA with mCherry

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for sgRNA expression and EFS promoter…

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pmScarlet_NES_C1

    Plasmid

    #85060

    Purpose

    In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pC1-REX-YFP

    Plasmid

    #48247

    Purpose

    Genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

    Depositor

    Vsevolod Belousov

    Article

    Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

    Insert

    REX-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBbS5a-Opto-Cre-Vvd-2

    Plasmid

    #160400

    Purpose

    Updated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

    Depositor

    Mary Dunlop

    Article

    Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

    Insert

    Opto-Cre-Vvd-2
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. [UBC][NRE Pum][eGFP]

    Plasmid

    #97192

    Purpose

    The wild-type Pum, fused to eGFP, and behind the ubiquitin promoter.

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Insert

    wild type Pumilio homology domain
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    UBC (ubiquitin C)

    Available Since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCytERM_mScarlet-i_N1

    Plasmid

    #85068

    Purpose

    In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    CytERM
    

    Use

    Tags

    mScarlet-i

    Expression

    Mammalian

    Mutation

    aa1-29

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFRT_TO_FlagHA_PRKRA

    Plasmid

    #106111

    Purpose

    Inducible gene expression of FlagHA-PRKRA

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    PRKRA (PRKRA Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AAVS1-idCas9-vpr

    Plasmid

    #89985

    Purpose

    inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus

    Depositor

    Jie Na

    Article

    Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/

    Insert

    dCas9-VPR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. 2.2

    Plasmid

    #34885

    Depositor

    Irvin Chen

    Article

    Pariente et al Mol Ther. 2007 Nov;15(11):1973-81. Epub

    Insert

    Sindbis virus envelope protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    SGN UTR in E1 domain

    Promoter

    CMV

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge