We narrowed to 34,434 results for: CaS

1. 3xNLS-NLP-cMyc-cMyc enAspCas12a

   Plasmid

   #182127

   Purpose

   pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   3xNLS-NLP-cMyc-cMyc enAspCas12a
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AOI-WT-Cas9-sg-mouse Suz12-F2-GFP

   Plasmid

   #91882

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Suz12 (Suz12 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pZLCv2-gD4Z4-1-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106352

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   D4Z4 gRNA-1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR

   Plasmid

   #81206

   Purpose

   CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   pGinB-8X(GGS)-dCas9-FLAG-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMD19T-psba1-Ppsba2-dCas9-SpR

   Plasmid

   #73220

   Purpose

   Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

   Depositor

   Paul Hudson

   Article

   Yao et al ACS Synth Biol. 2015 Dec 28.

   Insert

   dCas9 from S. pyogenes
   

   Use

   Tags

   c-myc

   Expression

   Bacterial

   Mutation

   Silent mutation at bp 1341 A->C to remove an E…

   Promoter

   PpsbA2

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX399 Mycoplasma gallisepticum str. F Cas9

   Plasmid

   #68335

   Purpose

   Mycoplasma gallisepticum str. F Cas9

   Depositor

   Feng Zhang

   Insert

   MgCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Construct 7 - CMVp-Cas9-3xNLS-HSVpA

   Plasmid

   #81247

   Purpose

   Expresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian cell expression

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   Cas9-3xNLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKEW321-MbCas12a-RFP-GFP-array

   Plasmid

   #115661

   Purpose

   Encodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   GFP and RFP array for MbCas12
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMSCV-IRES-GFP-bat-Caspase-4

   Plasmid

   #183377

   Purpose

   Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-4
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106355

    Purpose

    CRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locus

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    MYOD1 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 3xNLS-NLP-cMyc-cMyc AspCas12a

    Plasmid

    #182122

    Purpose

    pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    3xNLS-NLP-cMyc-cMyc AspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Px458-Cas9n-Trp63-Exon4-2sgRNA

    Plasmid

    #88849

    Purpose

    CRISPR KO of Trp63

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp63 (Trp63 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

13. pMS28: pTns(PmcCAST)_ΔTniQ,ΔTnsD

    Plasmid

    #168161

    Purpose

    Inducible expression of PmcCAST TnsAB and TnsC proteins.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    PmcCAST Tns proteins (TnsAB and TnsC)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. IA201: pMAGIC (R4-R3) NLS-Sa dCas9-NLS

    Plasmid

    #121821

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-dCas9 (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUPD2 nickase D10A Cas9 (GB1690)

    Plasmid

    #160587

    Purpose

    Nickase D10A Cas9 for Nt and Ct fusion.

    Depositor

    Diego Orzaez

    Insert

    nickase D10A Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6

    Plasmid

    #159913

    Purpose

    Mutagenesis of Kcnj6

    Depositor

    Larry Zweifel

    Insert

    Kcnj6 (Kcnj6 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCSDest2-SpCas9-NLS-3XHA-NLS

    Plasmid

    #69220

    Purpose

    Expresses wild type SpCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. lenti SYN-SVI-dCas9-VPR

    Plasmid

    #164575

    Purpose

    Expresses an intron-containing dCas9-VPR fusion driven by human SYN promoter

    Depositor

    Jeremy Day

    Article

    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

    Insert

    FLAG-dCas9-VPR containing an SV40 intron
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330-Flag-wtSpCas9 (without sgRNA)

    Plasmid

    #92353

    Purpose

    Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFlag-NLS-wild-type SpCas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106353

    Purpose

    CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    D4Z4 gRNA-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Mesp-1916/-1>nls::Cas9::nls

    Plasmid

    #59988

    Purpose

    Mesp driver driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Mesp -1916/-1

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. RCAS(A) human p110 alpha E545K

    Plasmid

    #52211

    Purpose

    expresses human p110 alpha with E545K mutation in avian cells

    Depositor

    Peter Vogt

    Article

    Kang et al Proc Natl Acad Sci U S A. 2005 Jan 18;10

    Insert

    p110 alpha E545K (PIK3CA Human)
    

    Use

    Retroviral; Avian

    Tags

    Expression

    Mutation

    Glutamic Acid 545 to Lysine

    Promoter

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBDKRB2_TCS(Q’L)_HA-dCas9(N)_DHFR

    Plasmid

    #101109

    Purpose

    Encodes dCas9(N)-DHFR fused to BDKRB2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    BDKRB2-dCas9(N)-DHFR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Px458-Cas9n-Trp73-Exon4-3sgRNA

    Plasmid

    #88850

    Purpose

    CRISPR KO of Trp73

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp73 (Trp73 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

25. Px458-Cas9n-Trp73-Exon4-4sgRNA

    Plasmid

    #88851

    Purpose

    CRISPR KO of Trp73

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp73 (Trp73 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

26. pET28b-NmCas9-Flag D16A+H588A

    Plasmid

    #109033

    Purpose

    D16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Use

    Tags

    Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Hy_pMT Laccase2 MCS-HIPK3 Exon

    Plasmid

    #69888

    Purpose

    Expresses the human HIPK3 exon 2 circular RNA in Drosophila

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    HIPK3 (HIPK3 Fly, Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Expresses aa1-194 of HIPK3

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. RCAS (B) flag Lunatic Fringe (CT#471)

    Plasmid

    #13980

    Depositor

    Cliff Tabin

    Article

    Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

    Insert

    Lunatic Fringe (LFNG Chicken)
    

    Use

    Retroviral; Avian expression

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

29. RCAS (A) flag Lunatic Fringe (CT#479)

    Plasmid

    #13979

    Depositor

    Cliff Tabin

    Article

    Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

    Insert

    Lunatic Fringe (LFNG Chicken)
    

    Use

    Retroviral; Avian expression

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

30. PB_Mut(D1399Y)_p300_core-dCas9-EGFP_hygro

    Plasmid

    #226427

    Purpose

    PiggyBac transposon vector containing a dox inducible mutant p300 core-dCas9 fusion protein (p300 core mutation: D1399Y) and EGFP reporter. Hygromycin selection marker.

    Depositor

    Alon Goren

    Inserts

    Mutant p300 core-dCas9-EGFP fusion protein
    rtTA3-IRES-Hygro
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    HA tag, Mutant p300 core, and P2A-EGFP

    Expression

    Mammalian

    Mutation

    p300 core mutation (D1399Y)

    Promoter

    Dox inducible minimal CMV and UbC Promoter

    Available since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-EFS-DjCas13d-T2A-mCherry-pA

    Plasmid

    #192499

    Purpose

    To express DjCas13d

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

    Insert

    DjCas13d
    

    Use

    AAV

    Tags

    Expression

    Mutation

    No

    Promoter

    EFS

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-U6-DR30(SapI)-0.5Syn-CasRx-pA

    Plasmid

    #192492

    Purpose

    To express CasRX and a gRNA

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

    Insert

    CasRx
    

    Use

    AAV

    Tags

    Expression

    Mutation

    No

    Promoter

    0.5Syn

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-EFS-CasRx-T2A-mCherry-pA

    Plasmid

    #192494

    Purpose

    To express CasRx

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

    Insert

    CasRx
    

    Use

    AAV

    Tags

    Expression

    Mutation

    No

    Promoter

    EFS

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-EFS-hfCas13d-T2A-mCherry-pA

    Plasmid

    #192497

    Purpose

    To express hfCas13d

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

    Insert

    high fidelity (hf) version of CasRx (hfCas13d)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Yes: GTGgaatacattaccaacGTGGTGtacGTG

    Promoter

    EFS

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-CMV-intron-DIO-DjCas13d-pA

    Plasmid

    #192498

    Purpose

    To express DjCas13d in a Cre dependent manner

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

    Insert

    DjCas13d
    

    Use

    AAV

    Tags

    Expression

    Mutation

    No

    Promoter

    CMV

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-0.4CaMKII-CasRx-T2A-mCherry-pA

    Plasmid

    #192483

    Purpose

    To express CasRx

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

    Insert

    CasRx
    

    Use

    AAV

    Tags

    Expression

    Mutation

    No

    Promoter

    0.4CaMKIIa

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-EF1a-CasRx-T2A-mCherry-pA

    Plasmid

    #192484

    Purpose

    To express CasRx

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

    Insert

    CasRx
    

    Use

    AAV

    Tags

    Expression

    Mutation

    No

    Promoter

    Ef1a

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSPcas9(BB)-2A-Puro V2.0 sgCHMP2B-2 aauucccaaaugaagauggc

    Plasmid

    #231998

    Purpose

    Expression of an sgRNA targeting CHMP2B, Cas9, and a PuroR marker

    Depositor

    Franz-Ulrich Hartl

    Insert

    CHMP2B-targeted sgRNA (CHMP2B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

39. pCMV-MMLVgag-D3A-3L-dCas9-KOX1

    Plasmid

    #240532

    Purpose

    Expresses MMLVgag–DNMT3A-3L-dCas9-KOX1 for producing RENDER-DNMT3A-3L-dCas9-KOX1

    Depositor

    James Nuñez

    Article

    Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

    Insert

    MMLVgag-D3A-3L-dCas9-KOX1
    

    Use

    Tags

    FLAG, HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Lenti-E14-ABE-Cas9n-puro

    Plasmid

    #226588

    Purpose

    Express E14-ABE in mammalian cells

    Depositor

    Gang Wang

    Article

    Zhao et al Nat Commun. 2024 Sep 17;15(1):8140. doi:

    Insert

    E14-ABE
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. nzCas9n-GSG-linker-P2A-mNeongreen

    Plasmid

    #241189

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pGloSensor -30F DEVD Caspase Vector

    Plasmid

    #236880

    Purpose

    Express -30F DEVD for Caspase 3/7 assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    -30F DEVD
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. AAV-pCamKII-Cre-pU6-SpCas9gRNAentry

    Plasmid

    #210391

    Purpose

    AAV plasmid encoding the CamKII promoter driving Cre expression, along with SpCas9 gRNA entry cassette (RFP dropout)

    Depositor

    Benjamin Kleinstiver

    Article

    Huynh et al Nature. 2024 Nov;635(8037):168-177. doi:

    Insert

    AAV-(ITR)-pCamKII-NLS-Cre-WPRE-bGHpA-(rev)-SpCas9_gRNA-BsmBI_RFPcassette-hU6-(ITR) (NJA158)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII promoter for Cre, human U6 promoter for Sp…

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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44. iC30 PB-Zim3-dCas9-P2A-BFP_hygro

    Plasmid

    #236743

    Purpose

    Constitutive expression of Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    CAG-Zim3-NLS-dCas9-HA-P2A-2xmycNLS-tagBFP-WPRE
    EF1a-hygro-WPRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1a

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)

    Plasmid

    #223111

    Purpose

    pCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCAG-SpCas9-P2A-EGFP (MSP2582)

    Plasmid

    #223067

    Purpose

    pCAG human codon optimized wild-type SpCas9 expression plasmid with C-terminal NLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized SpCas9-P2A-EGFP
    

    Use

    CRISPR

    Tags

    NLS(SV40)-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. T7-RBS-6xHis-SUMO-SynCas.v10 bacteria

    Plasmid

    #208594

    Purpose

    Production and purification of catalytically active SynCas.v10

    Depositor

    Omar Akbari

    Article

    Brogan et al bioRxiv 2024.02.23.581838; doi: https://

    Insert

    SynCas.v10
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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48. T7-RBS-6xHis-SUMO-dSynCas.v10 bacteria

    Plasmid

    #208595

    Purpose

    Production and purification of catalytically inactive SynCas.v10

    Depositor

    Omar Akbari

    Article

    Brogan et al bioRxiv 2024.02.23.581838; doi: https://

    Insert

    dSynCas.v10
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. T7-RBS-6xHis-SUMO-SynCas.v12 bacteria

    Plasmid

    #208596

    Purpose

    Production and purification of catalytically active SynCas.v12

    Depositor

    Omar Akbari

    Article

    Brogan et al bioRxiv 2024.02.23.581838; doi: https://

    Insert

    SynCas.v12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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50. T7-RBS-6xHis-SUMO-dSynCas.v12 bacteria

    Plasmid

    #208597

    Purpose

    Production and purification of catalytically inactive SynCas.v12

    Depositor

    Omar Akbari

    Article

    Brogan et al bioRxiv 2024.02.23.581838; doi: https://

    Insert

    dSynCas.v12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge