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pCDNA3.1- dCas9-2xNLS-EGFP

Plasmid

#74710

Purpose

Expresses dCas9-2xNLS-EGFP in mammalian cells

Depositor

Eugene Yeo

Publication

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-2xNLS-EGFP

Use

CRISPR

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Cas9 sgRNA vector

Plasmid

#68463

Purpose

Cas9 sgRNA vector for cloning

Depositor

Su-Chun Zhang

Publication

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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mEos4b-N1

Plasmid

#54814

Purpose

Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Publication

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Type

Empty backbone

Tags

mEos4b

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pcDNA3 HA eIF4GI (1-1599)

Plasmid

#45640

Depositor

Nahum Sonenberg

Publication

Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

Insert

eIF4GI transcript variant 2 (EIF4G1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3-mNeptune2.5

Plasmid

#51310

Purpose

Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells

Depositor

Michael Lin

Publication

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mNeptune2.5

Expression

Mammalian

Promoter

CMV

Available since

April 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-hSyn-DIO-EGFP

Plasmid

#50457

Purpose

Double floxed EGFP under the control of human synapsin promoter

Depositor

Bryan Roth

Has service

AAV1, AAV2, AAV5, AAV8, AAV9, and AAV Retrograde

Insert

EGFP

Use

AAV

Tags

N/A

Promoter

human Synapsin 1

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

128 Articles

1 Data Hub Report

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AmCyan-P2A-mCherry

Plasmid

#45350

Depositor

Ilpo Huhtaniemi

Publication

Potorac et al J Endocrinol. 2016 Sep 21. pii: JOE-16-0

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AmCyan

Tags

mCherry

Expression

Mammalian

Mutation

SV40 NLS

Promoter

CMV

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Lenti-sh1368 knockdown c-myc

Plasmid

#29435

Depositor

Bob Eisenman

Publication

Lin et al EMBO J. 2009 Oct 21. 28(20):3157-70.

Insert

c-Myc shRNA (MYC Human)

Use

Lentiviral and RNAi

Tags

GFP and WPRE

Expression

Mammalian

Available since

May 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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EGFP-Rab5A Q79L

Plasmid

#28046

Depositor

Qing Zhong

Publication

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab5A (RAB5A Human)

Tags

EGFP on N-term

Expression

Mammalian

Mutation

Q79L

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pMXs-Hu-L-Myc

Plasmid

#26022

Depositor

Shinya Yamanaka

Publication

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

L-Myc (MYCL Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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MSCV

Plasmid

#24828

Depositor

Lin He

Publication

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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3XFlag pCMV5-TOPO TAZ WT

Plasmid

#24809

Depositor

Jeff Wrana

Publication

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Available since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

12 Articles

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12.4kbVillin-ΔATG

Plasmid

#19358

Depositor

Deborah Gumucio

Publication

Madison et al J Biol Chem. 2002 Sep 6. 277(36):33275-8

Insert

12.4kb Villin promoter

Expression

Bacterial

Mutation

No ATG - transgenes cloned downstream of the vill…

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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MSCV PIG (Puro IRES GFP)

Plasmid

#18751

Depositor

Scott Lowe

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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Lac-I-SceI-Tet

Plasmid

#17655

Depositor

Tom Misteli

Publication

Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

Insert

lac I-SceI tet

Expression

Bacterial

Available since

May 30, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
EF.PGK.GFP

Plasmid

#17618

Depositor

Linzhao Cheng

Publication

Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3-DN-hCUL4A-FLAG

Plasmid

#15821

Depositor

Wade Harper

Publication

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cullin4A (CUL4A Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N440

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pSV Sport SREBP-1c

Plasmid

#8883

Depositor

Bruce Spiegelman

Publication

Tontonoz et al Mol Cell Biol. 1993 Aug . 13(8):4753-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SREBP-1c (Srebf1 Mouse)

Expression

Mammalian

Mutation

Active version of SREBP-1c.

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag

Plasmid

#132398

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLVpuro-CMV-N-3xFLAG

Plasmid

#123223

Purpose

N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pmGFP-P2A-K0-P2A-RFP

Plasmid

#105686

Purpose

Double fluorescent stalling reporter K0

Depositor

Ramanujan Hegde

Publication

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

P2A-3xFLAG-VHPbeta
K0
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pTP1183

Plasmid

#104163

Purpose

Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)

Depositor

Dirk Görlich

Publication

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pFC332

Plasmid

#87845

Purpose

AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

Depositor

Uffe Mortensen

Publication

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
hph (hygromycin resistance marker)

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and Aspergillu…

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pSG5-HA-p300

Plasmid

#89094

Purpose

mammalian expression of full-length human p300 in pSG5

Depositor

Elizabeth Wilson

Publication

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

HA

Expression

Mammalian

Promoter

SV40

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pMIR-FLAG-MLL-AF9

Plasmid

#71444

Purpose

Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

Depositor

Daisuke Nakada

Publication

Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

Insert

MLL-AF9 (KMT2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

MSCV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCS6-YFP-SMASh

Plasmid

#68853

Purpose

Expresses SMASh tag fused to YFP at C terminus in mammalian cells

Depositor

Michael Lin

Publication

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

YFP-SMASh

Tags

SMASh tag

Expression

Mammalian

Promoter

CMV

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

Plasmid

#64218

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A

Depositor

Ralf Kuehn

Publication

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP-P2A-E1B

Expression

Mammalian

Promoter

CBh and U6

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLV_hEF1a_rtTA3

Plasmid

#61472

Purpose

3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

Depositor

Ron Weiss

Publication

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Insert

rtTA3

Use

Lentiviral

Promoter

hEF1a

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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2C::tdTomato Reporter

Plasmid

#40281

Depositor

Samuel Pfaff

Publication

Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

2C Promoter (2-cell stage promoter)

Tags

tdTomato

Expression

Mammalian

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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LeGO-iG2

Plasmid

#27341

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, IRES-eGFP

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles

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