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Showing: 3041 - 3080 of 5015 results

  1. pcDNA3-HCMVgO-8his (Merlin)

    Plasmid
    #136454
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) with an 8his tag
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    glycoprotein O (UL74 Human betaherpesvirus 5)
    Use
    Tags
    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136454-plasmid-map-sequence-id-269878

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  6. VQR variant

    Plasmid
    #138560
    Purpose
    Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VQR
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138560-plasmid-map-sequence-id-274782

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  8. pJR2955

    Plasmid
    #122550
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemNeptune2.5) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemNeptune2.5
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122550-plasmid-map-sequence-id-238124

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  9. MSP2372

    Plasmid
    #73196
    Purpose
    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, and Q926A in SpCas9
    Promoter
    CMV
    Available since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73196-plasmid-map-sequence-id-134659

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  13. GB1-PRD(703-868, P801G)-Strep

    Plasmid
    #164444
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G)-Strep.
    Depositor
    Lalit Deshmukh
    Article
    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10
    Insert
    GB1-His6-TEV-ALIX(703-868, P801G)-Strep (PDCD6IP Human)
    Use
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Proline 801 to Glycine
    Promoter
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    164444-plasmid-map-sequence-id-324031

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  19. pJR2953

    Plasmid
    #122549
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemCardinal2) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemCardinal2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122549-plasmid-map-sequence-id-238078

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  22. pLenti_dCas9-2xAM_hIRF-1

    Plasmid
    #92221
    Purpose
    Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter
    Depositor
    Hodaka Fujii
    Article
    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do
    Inserts
    Sp-dCas9-2xAM tag
    gRNA targeting human IRF-1 promoter
    Use
    CRISPR and Lentiviral
    Tags
    2xAM tag
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    CBh and U6
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    92221-plasmid-map-sequence-id-181086

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  23. pEPQD1CB0202

    Plasmid
    #185626
    Purpose
    MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    SpCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    185626-plasmid-map-sequence-id-368553

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  28. pSHDY*in_PpetE:BCD2:mVenus

    Plasmid
    #133970
    Purpose
    Heterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.
    Depositor
    Pia Lindberg
    Article
    Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.
    Insert
    mVenus
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    inserted gene is codon optimized for Synechocyst…
    Promoter
    PpetE:BCD2
    Available since
    April 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133970-plasmid-map-sequence-id-267750

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  33. pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)

    Plasmid
    #160635
    Purpose
    TU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminator
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4
    Insert
    AcrIIA4
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    35S
    Available since
    Sept. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    160635-plasmid-map-sequence-id-427149

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  34. pRAN357

    Plasmid
    #120898
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.
    Insert
    cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ptet
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120898-plasmid-map-sequence-id-235310

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  36. pYPQ291

    Plasmid
    #129671
    Purpose
    Gateway entry clone for CRISPR-Cas12b systems
    Depositor
    Yiping Qi
    Article
    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:
    Insert
    BthCas12b
    Use
    CRISPR; Gateway compatible bthcas12b entry clone
    Tags
    Expression
    Plant
    Mutation
    BthCas12b is rice codon optimized
    Promoter
    Available since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    129671-plasmid-map-sequence-id-255057

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Showing: 3041 - 3080 of 5015 results